Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance

Liu, Xintong; Li, Dandan; Zhang, Shiya; Xu, Yaling; Zhang, Zhao

doi:10.1186/s12870-019-2139-6

Cited by 27 publications

(25 citation statements)

References 54 publications

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“…The completion of monthly genome-wide sequencing provides a guarantee for increasing monthly salt resistance through molecular breeding (Bourke et al, 2018;Liu et al, 2019). Indeed, a number of studies showed that in alkaline saline soil plants can withstand salt stress while resisting high pH stress.…”

Section: Core Ideasmentioning

confidence: 99%

Comparative transcriptome analysis of Rosa chinensis ‘Old Blush’ provides insights into the crucial factors and signaling pathways in salt stress response

Bao

Chen

et al. 2021

Agronomy Journal

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The growth, development, and quality production of Rosa hybrida L. are the core issues for being able to have good yields. Inadequate information on genetic background and resistance has hindered the ability to continue improving flower quality, and most cultivars continue to have lower‐than‐desired levels of resistance. Therefore, this study sequenced the transcription group of the R. chinensis Jacq. ‘Old Blush’ under high salt stress. Transcription group sequencing was carried out on root and leaf materials at different points in time (0, 2, 24, and 48 h) under a high salt stress condition of NaHCO3. The monthly salt stress recorded within 48‐h Ca2+ sensors was mainly calmodulin and calcium‐binding protein. The kinase 5 (RC2G0184500) began to show significant increase from 24‐ and 48‐h process time, respectively, within the leaf blades and the root. There was an increase of superoxide dismutase and L‐ascorbate peroxidase from the 2‐ and 48‐h processing times, respectively. Within the blades, MYB4, MYB41, MYB44, MYB20, MYB62, and MYB14 had a log2Foldchange of >4. ERF109 had a log2Foldchange of 7. NAC‐like transcription factors had more differential genes in the root than in the leaves, with a log2Foldchange of NAC67, NAC52, NAC16, and NAC2 of >4. The plant hormone signal transduction‐related genes involved were abscisic acid, growth hormone, ethylene, erythromycin, jasmine acid, salbonate, and cell division. The EIN4 and EIN3 were only raised in the leaf and the serine/threonine‐protein kinase CTR1 was raised in the root, whereas the ethylene pathway was mainly in the leaves. Auxin‐responsive protein SAUR72 and SAUR50 were raised in the leaves and the other auxin‐responsive protein and SAUR expression in the root were low. The IAA2 was revised upward in the leaf and the IAA11 was revised upward in the root, whereas the rest were revised downward. In conclusion, the study revealed several genes and hormones that are key in salt stress response.

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Section: Core Ideasmentioning

confidence: 99%

Comparative transcriptome analysis of Rosa chinensis ‘Old Blush’ provides insights into the crucial factors and signaling pathways in salt stress response

Bao

Chen

et al. 2021

Agronomy Journal

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“…This has been recently done for MLO genes in various species, including soybean (Deshmukh et al 2014 ), Cicer arietinum and Medicago truncatula (Deshmukh et al 2017 ), Populus trichocarpa (Filiz and Vatansever 2018 ), pumpkin (Win et al 2018 ), lentil (Polanco et al 2018 ), bitter gourd (Chen et al 2021 ), for the promotor region of MLO genes in melon and other species (Andolfo et al 2019 ), and for other susceptibility genes (Pirrello et al 2021 ). In rose overviews have been generated for WRKY genes (Liu et al 2019 ), NBS-LRR genes (Lopez Arias et al 2020 ), the disease resistance gene family Rdr1 (Menz et al 2020 ), AP2/ERF transcription factors (Li et al 2021 ), and S-RNases and F-box genes involved in self-incompatibility (Vieira et al 2021 ). These inventories may be used as a basis to find or generate loss-of-function alleles of relevant MLO genes.…”

Section: Introductionmentioning

confidence: 99%

Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs

et al. 2021

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Key message Rose has 19 MLO genes. Of these, RhMLO1 and RhMLO2 were shown to be required for powdery mildew infection, which suggests their potential as susceptibility targets towards disease resistance. Abstract Powdery mildew, caused by Podosphaera pannosa, is one of the most serious and widespread fungal diseases for roses, especially in greenhouse-grown cut roses. It has been shown that certain MLO genes are involved in powdery mildew susceptibility and that loss of function in these genes in various crops leads to broad-spectrum, long-lasting resistance against this fungal disease. For this reason, these MLO genes are called susceptibility genes. We carried out a genome-wide identification of the MLO gene family in the Rosa chinensis genome, and screened for allelic variants among 22 accessions from seven different Rosa species using re-sequencing and transcriptome data. We identified 19 MLO genes in rose, of which four are candidate genes for functional homologs in clade V, which is the clade containing all dicot MLO susceptibility genes. We detected a total of 198 different allelic variants in the set of Rosa species and accessions, corresponding to 5–15 different alleles for each of the genes. Some diploid Rosa species shared alleles with tetraploid rose cultivars, consistent with the notion that diploid species have contributed to the formation of tetraploid roses. Among the four RhMLO genes in clade V, we demonstrated using expression study, virus-induced gene silencing as well as transient RNAi silencing that two of them, RhMLO1 and RhMLO2, are required for infection by P. pannosa and suggest their potential as susceptibility targets for powdery mildew resistance breeding in rose.

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“…To elucidate the roles of WRKYs in different species and improve the current understanding of biotic and abiotic stress responses at the molecular level, the identification and analysis of WRKY family members in target plant species is essential. Studies on the WRKY TF family in rice (Ross, Liu & Shen, 2007;Ryu et al, 2006), Glycyrrhiza glabra (Goyal et al, 2020), Rosa chinensis (Liu et al, 2019), and Saccharum spontaneum have already been conducted. These studies reveal that the WRKY TF family is crucially involved in biotic and abiotic stress responses and that some of these genes have been produced by duplication events.…”

Section: Introductionmentioning

confidence: 99%

The whole-genome and expression profile analysis of WRKY and RGAs in Dactylis glomerata showed that DG6C02319.1 and DgWRKYs may cooperate in the immunity against rust

Ren

Zhang

et al. 2021

PeerJ

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Orchardgrass (Dactylis glomerata) is one of the top four perennial forages worldwide and, despite its large economic advantages, often threatened by various environmental stresses. WRKY transcription factors (TFs) can regulate a variety of plant processes, widely participate in plant responses to biotic and abiotic stresses, and are one of the largest gene families in plants. WRKYs can usually bind W-box elements specifically. In this study, we identified a total of 93 DgWRKY genes and 281 RGAs, including 65, 169 and 47 nucleotide-binding site-leucine-rich repeats (NBS-LRRs), leucine-rich repeats receptor-like protein kinases (LRR-RLKs), and leucine-rich repeats receptor-like proteins (LRR-RLPs), respectively. Through analyzing the expression of DgWRKY genes in orchardgrass under different environmental stresses, it was found that many DgWRKY genes were differentially expressed under heat, drought, submergence, and rust stress. In particular, it was found that the greatest number of genes were differentially expressed under rust infection. Consistently, GO and KEGG enrichment analysis of all genes showed that 78 DgWRKY TFs were identified in the plant–pathogen interaction pathway, with 59 of them differentially expressed. Through cis-acting element prediction, 154 RGAs were found to contain W-box elements. Among them, DG6C02319.1 (a member of the LRR-RLK family) was identified as likely to interact with 14 DGWRKYs. Moreover, their expression levels in susceptible plants after rust inoculation were first up-regulated and then down-regulated, while those in the resistant plants were always up-regulated. In general, DgWRKYs responded to both biotic stress and abiotic stress. DgWRKYs and RGAs may synergistically respond to the response of orchardgrass to rust. This study provides meaningful insight into the molecular mechanisms of WRKY proteins in orchardgrass.

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Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance

Cited by 27 publications

References 54 publications

Comparative transcriptome analysis of Rosa chinensis ‘Old Blush’ provides insights into the crucial factors and signaling pathways in salt stress response

Comparative transcriptome analysis of Rosa chinensis ‘Old Blush’ provides insights into the crucial factors and signaling pathways in salt stress response

Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs

The whole-genome and expression profile analysis of WRKY and RGAs in Dactylis glomerata showed that DG6C02319.1 and DgWRKYs may cooperate in the immunity against rust

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