Genome-Wide Characterization of the RNA Exosome Complex in Relation to Growth, Development, and Pathogenicity of Fusarium graminearum

Yuan, Yanping; Mao, Xuzhao; Abubakar, Yakubu Saddeeq; Zheng, Wenhui; Wang, Zehua; Zhou, Jie; Zheng, Huawei

doi:10.1128/spectrum.05058-22

Cited by 4 publications

(6 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These downregulated proteins were implicated in a wide range of biological processes, including translation, ribosome biogenesis, the cell cycle, oxidative phosphorylation and TCA cycle, protein processing in endoplasmic reticulum, and exosome ( Supplementary Tables 4 , 5 ). Abnormalities of these cellular processes jeopardize cell growth and functions, from energy metabolism to pathogenicity ( Fernández-Álvarez et al, 2013 ; Koh and Sarin, 2018 ; Richardson, 2019 ; Zhao et al, 2021 ; Yuan et al, 2023 ). Of the SUMOylated proteins, CpSep1, CpSlt2, CpMK2, CpMK1, and Cdc48 have been shown to be virulence factors in C. parasitica ( Park et al, 2004 ; Choi et al, 2005 ; Ko et al, 2016 ; So et al, 2017 ; Jo et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

CpSmt3, an ortholog of small ubiquitin-like modifier, is essential for growth, organelle function, virulence, and antiviral defense in Cryphonectria parasitica

Li,

Chen,

Wei

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionSUMOylation is an important post-translational modification that regulates the expression, localization, and activity of substrate proteins, thereby participating in various important cellular processes such as the cell cycle, cell metabolism, gene transcription, and antiviral activity. However, the function of SUMOylation in phytopathogenic fungi has not yet been adequately explored.MethodsA comprehensive analysis composed of proteomics, affinity pull-down, molecular and cellular approaches was performed to explore the roles of SUMOylation in Cryphonectria parasitica, the fungal pathogen responsible for chestnut blight.Results and discussionCpSmt3, the gene encoding the SUMO protein CpSmt3 in C. parasitica was identified and characterized. Deletion of the CpSmt3 gene resulted in defects in mycelial growth and hyphal morphology, suppression of sporulation, attenuation of virulence, weakening of stress tolerance, and elevated accumulation of hypovirus dsRNA. The ΔCpSmt3 deletion mutant exhibited an increase in mitochondrial ROS, swollen mitochondria, excess autophagy, and thickened cell walls. About 500 putative SUMO substrate proteins were identified by affinity pull-down, among which many were implicated in the cell cycle, ribosome, translation, and virulence. Proteomics and SUMO substrate analyses further revealed that deletion of CpSmt3 reduced the accumulation of CpRho1, an important protein that is involved in TOR signal transduction. Silencing of CpRho1 resulted in a phenotype similar to that of ΔCpSmt3, while overexpression of CpRho1 could partly rescue some of the prominent defects in ΔCpSmt3. Together, these findings demonstrate that SUMOylation by CpSmt3 is vitally important and provide new insights into the SUMOylation-related regulatory mechanisms in C. parasitica.

show abstract

Section: Discussionmentioning

confidence: 99%

CpSmt3, an ortholog of small ubiquitin-like modifier, is essential for growth, organelle function, virulence, and antiviral defense in Cryphonectria parasitica

Li,

Chen,

Wei

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The total proteins (Input) and the proteins eluted from the agarose beads (IP) were analyzed by western blot using the anti-Myc and anti-GFP antibodies, respectively. 17 Quantification of Acetyl-CoA. Acetyl-CoA was assayed as previously reported.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Total proteins were extracted from the strain expressing the pair of fusion constructs and were further incubated with the anti-GFP agarose beads (SM038001, Smart-Lifesciences) at 4 °C for 4 h following the standard procedure. The total proteins (Input) and the proteins eluted from the agarose beads (IP) were analyzed by western blot using the anti-Myc and anti-GFP antibodies, respectively …”

Section: Materials and Methodsmentioning

confidence: 99%

“…These mycotoxins contaminate grains and cause serious health problems in humans and animals. − In Fusarium species, the enzymes involved in the de novo trichothecene biosynthetic pathway (from acetyl-CoA to DON) have been comprehensively investigated. − Two cytochrome P-450 oxygenases (FgTri1 and FgTri4) localize to spherical organelles, approximately 3 to 4 μm in diameter, that were presumed to be the site for trichothecene biosynthesis. ,, Subsequently, these spherical organelles were named toxisomes, derived from remodeled endoplasmic reticulum (ER) under mycotoxin-inducing conditions. , In recent years, myosin I, actin capping proteins, microtubule, plasma membrane H + -ATPase FgPMA1, venturicidin A, transcription factor FgStuA, and RNA exosome complex were demonstrated to be required for toxisome formation or assembly in F. graminearum. ,− However, the mechanisms of the formation and assembly of the toxisomes are still not well characterized.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nucleoside Diphosphate Kinase FgNdpk Is Required for DON Production and Pathogenicity by Regulating the Growth and Toxisome Formation of Fusarium graminearum

Mao,

Li,

Abubakar

et al. 2024

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Nucleoside diphosphate kinases (NDPKs) are nucleotide metabolism enzymes that play different physiological functions in different species. However, the roles of NDPK in phytopathogen and mycotoxin production are not well understood. In this study, we showed that Fusarium graminearum FgNdpk is important for vegetative growth, conidiation, sexual development, and pathogenicity. Furthermore, FgNdpk is required for deoxynivalenol (DON) production; deletion of FgNDPK downregulates the expression of DON biosynthesis genes and disrupts the formation of FgTri4-GFP-labeled toxisomes, while overexpression of FgNDPK significantly increases DON production. Interestingly, FgNdpk colocalizes with the DON biosynthesis proteins FgTri1 and FgTri4 in the toxisome, and coimmunoprecipitation (Co-IP) assays show that FgNdpk associates with FgTri1 and FgTri4 in vivo and regulates their localizations and expressions, respectively. Taken together, these data demonstrate that FgNdpk is important for vegetative growth, conidiation, and pathogenicity and acts as a key protein that regulates toxisome formation and DON biosynthesis in F. graminearum.

show abstract

“…The deletion mutant of Fgexosc1 was unable to form perithecia. Although the deletion mutant of FgexoscA exhibited normal perithecia formation, it showed a significant reduction in the quantity of ascospores generated compared to the wild-type strain PH-1 [50] (Supplementary Table S1).…”

Section: Genes Involves In the Formation Of Peritheciamentioning

confidence: 99%

Advances in Understanding Fusarium graminearum: Genes Involved in the Regulation of Sexual Development, Pathogenesis, and Deoxynivalenol Biosynthesis

Niu,

Yang,

Liao

et al. 2024

Genes

View full text Add to dashboard Cite

The wheat head blight disease caused by Fusarium graminearum is a major concern for food security and the health of both humans and animals. As a pathogenic microorganism, F. graminearum produces virulence factors during infection to increase pathogenicity, including various macromolecular and small molecular compounds. Among these virulence factors, secreted proteins and deoxynivalenol (DON) are important weapons for the expansion and colonization of F. graminearum. Besides the presence of virulence factors, sexual reproduction is also crucial for the infection process of F. graminearum and is indispensable for the emergence and spread of wheat head blight. Over the last ten years, there have been notable breakthroughs in researching the virulence factors and sexual reproduction of F. graminearum. This review aims to analyze the research progress of sexual reproduction, secreted proteins, and DON of F. graminearum, emphasizing the regulation of sexual reproduction and DON synthesis. We also discuss the application of new gene engineering technologies in the prevention and control of wheat head blight.

show abstract

Genome-Wide Characterization of the RNA Exosome Complex in Relation to Growth, Development, and Pathogenicity of Fusarium graminearum

Abstract: The RNA exosome complex is the most versatile RNA degradation machinery in eukaryotes. However, little is known about how this complex regulates the development and pathogenicity of plant-pathogenic fungi.

Cited by 4 publications

References 76 publications

CpSmt3, an ortholog of small ubiquitin-like modifier, is essential for growth, organelle function, virulence, and antiviral defense in Cryphonectria parasitica

CpSmt3, an ortholog of small ubiquitin-like modifier, is essential for growth, organelle function, virulence, and antiviral defense in Cryphonectria parasitica

Nucleoside Diphosphate Kinase FgNdpk Is Required for DON Production and Pathogenicity by Regulating the Growth and Toxisome Formation of Fusarium graminearum

Advances in Understanding Fusarium graminearum: Genes Involved in the Regulation of Sexual Development, Pathogenesis, and Deoxynivalenol Biosynthesis

Contact Info

Product

Resources

About