Genome-wide characterization of JASMONATE-ZIM DOMAIN transcription repressors in wheat (Triticum aestivum L.)

Abstract

BackgroundThe JASMONATE-ZIM DOMAIN (JAZ) repressor family proteins are jasmonate co-receptors and transcriptional repressor in jasmonic acid (JA) signaling pathway, and they play important roles in regulating the growth and development of plants. Recently, more and more researches on JAZ gene family are reported in many plants. Although the genome sequencing of common wheat (Triticum aestivum L.) and its relatives is complete, our knowledge about this gene family remains vacant.ResultsFourteen JAZ genes were identified in the wheat genome. Structural analysis revealed that the TaJAZ proteins in wheat were as conserved as those in other plants, but had structural characteristics. By phylogenetic analysis, all JAZ proteins from wheat and other plants were clustered into 11 sub-groups (G1-G11), and TaJAZ proteins shared a high degree of similarity with some JAZ proteins from Aegliops tauschii, Brachypodium distachyon and Oryza sativa. The Ka/Ks ratios of TaJAZ genes ranged from 0.0016 to 0.6973, suggesting that the TaJAZ family had undergone purifying selection in wheat. Gene expression patterns obtained by quantitative real-time PCR (qRT-PCR) revealed differential temporal and spatial regulation of TaJAZ genes under multifarious abiotic stress treatments of high salinity, drought, cold and phytohormone. Among these, TaJAZ7, 8 and 12 were specifically expressed in the anther tissues of the thermosensitive genic male sterile (TGMS) wheat line BS366 and normal control wheat line Jing411. Compared with the gene expression patterns in the normal wheat line Jing411, TaJAZ7, 8 and 12 had different expression patterns in abnormally dehiscent anthers of BS366 at the heading stage 6, suggesting that specific up- or down-regulation of these genes might be associated with the abnormal anther dehiscence in TGMS wheat line.ConclusionThis study analyzed the size and composition of the JAZ gene family in wheat, and investigated stress responsive and differential tissue-specific expression profiles of each TaJAZ gene in TGMS wheat line BS366. In addition, we isolated 3 TaJAZ genes that would be more likely to be involved in the regulation of abnormal anther dehiscence in TGMS wheat line. In conclusion, the results of this study contributed some novel and detailed information about JAZ gene family in wheat, and also provided 3 potential candidate genes for improving the TGMS wheat line.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3582-0) contains supplementary material, which is available to authorized users.

Genome-Wide Analysis Reveals Stress and Hormone Responsive Patterns of JAZ Family Genes in Camellia Sinensis

“…Motifs 4 and 6 were specifically distributed in the N-terminal regions of the corresponding proteins. Motifs 7,8,10,11,12,13,14, and 15 were found distributed in N-terminal or C-terminal regions in different group members. Motifs 9 and 16 were mainly distributed in the C-terminal regions of the corresponding proteins, except CsJAZ5 and CsJAZ9.…”

Genome-wide and expression pattern analysis of JAZ family involved in stress responses and postharvest processing treatments in Camellia sinensis

“…Similarly, in the tomato, the SlJAZ7 and SlJAZ11 genes were significantly upregulated by ABA 43 . In wheat, the transcriptional levels of TaJAZ1 and 9 were significantly increased by GA treatment 44 . Moreover, the JAZ genes also exhibited distinct expression patterns under different hormone stresses in grapes and rice 45,46 .…”

“…In apples, overexpression of MdJAZ1 or MdJAZ4 weakened the promotive effect of MdMYC2 on cold tolerance 49 . In wheat, most of the TaJAZ genes were highly sensitive to drought and low-temperature treatment 44 . In this study, CsJAZ3, CsJAZ5-6, CsJAZ8 and CsJAZ12 expression was significantly up-regulated by cold stress, suggesting that these CsJAZ genes might be involved in the ICE-CBF/ DREB1 response to cold stress.…”

JAZ proteins modulate seed germination through interaction with ABI5 in bread wheat and Arabidopsis

“…To explore whether and how JA is involved in seed germination and/or in PHS in bread wheat, we identified a JAZ protein (herein called TaJAZ1), which is most closely related to Arabidopsis JAZ3 ( Fig. S1) (Wang et al, 2017). We then generated the pUbi:TaJAZ1DJas transgenic lines (OE7 and OE16) in bread wheat cv KN199 background overexpressing truncated TaJAZ1 (Jing et al, 2019), a stabilized form of TaJAZ1, in which the C-terminal Jas motif was deleted (Chini et al, 2007;Thines et al, 2007).…”