Genome-Wide Characterization of Glutathione Peroxidase (GPX) Gene Family in Rapeseed (Brassica napus L.) Revealed Their Role in Multiple Abiotic Stress Response and Hormone Signaling

Abstract

Plant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain H2O2 homeostasis and normalize plant reaction to abiotic stress conditions. To understand the major roles of the GPX gene family in rapeseed (Brassica napus L.), for the first time, a genome-wide study identified 25 BnGPX genes in the rapeseed genome. The phylogenetic analysis discovered that GPX genes were grouped into four major groups (Group I–Group IV) from rapeseed and three closely interrelated plant species. The universal investigation uncovered that the BnGPXs gene experienced segmental duplications and positive selection pressure. Gene structure and motifs examination recommended that most of the BnGPX genes demonstrated a comparatively well-maintained exon-intron and motifs arrangement within the identical group. Likewise, we recognized five hormones-, four stress-, and numerous light-reactive cis-elements in the promoters of BnGPXs. Five putative bna-miRNAs from two families were also prophesied, targeting six BnGPXs genes. Gene ontology annotation results proved the main role of BnGPXs in antioxidant defense systems, ROS, and response to stress stimulus. Several BnGPXs genes revealed boosted expression profiles in many developmental tissues/organs, i.e., root, seed, leaf, stem, flower, and silique. The qRT-PCR based expression profiling exhibited that two genes (BnGPX21 and BnGPX23) were suggestively up-regulated against different hormones (ABA, IAA, and MeJA) and abiotic stress (salinity, cold, waterlogging, and drought) treatments. In short, our discoveries provide a basis for additional functional studies on the BnGPX genes in future rapeseed breeding programs.

Genome-wide association mapping for high temperature tolerance in wheat through 90k SNP array using physiological and yield traits

“…The A genome relatively exhibited a low number of MTAs (47), but the B genome contained more MTAs (70), and the D genome have (52) MTAs in non-stressed conditions in the present study. The chromosome-wise observing, MTAs recorded on chromosomes more 2D (16), than 5A (13), 7A (13), 3B (11), 4B (11) and 3D (10) followed by 2B (10), 7B (8) and 7D (7) under heat stressed conditions. The B genome contained lowest MTAs (61), while the A and D genomes had same number (45) of MTAs under heat stressed conditions in the current experiment (Table 3).…”

“…Chromosome-wise, maximum MTAs were observed on chromosomes 7B (22), 4B (15), 5A (13), 2D (13) followed by 1D (11), 1B (10), 2B (9) and 7A (9) in non-stress conditions. The A genome relatively exhibited a low number of MTAs (47), but the B genome contained more MTAs (70), and the D genome have (52) MTAs in non-stressed conditions in the present study.…”

“…The demand for wheat continuously increasing with the constant growth of the human population and is expected to rise up to 40% till 2030. So, increased grain yield is an imperative need to establish food security [9][10][11]. Wheat cultivation ranks first between another cereal grain crops because it has more nutritional values and maximum uses.…”

Mechanistic Insights Into Trehalose-Mediated Cold Stress Tolerance in Rapeseed (Brassica napus L.) Seedlings

“…Most importantly, the effect of exogenous Tre in improving the CS tolerance in rapeseed ( Brassica napus L.) is still unclear. Rapeseed is the third-leading oilseed crop in China that is frequently exposed to CS, mainly in the winter ( He et al, 2021 ; Li et al, 2021 ; Raza, 2021 ; Su et al, 2021a ). The CS significantly impairs rapeseed productivity and ultimately reduces biomass and seed harvest ( Raza, 2021 ).…”

Genome-Wide Identification and Expression Profiling of DUF221 Gene Family Provides New Insights Into Abiotic Stress Responses in Potato

“…Almost 70% of annual potential crop yield losses are due to variations in the environment ( Leng et al, 2021 ). Abiotic factors are the major limiting stress factors that affect plants during their vegetative and reproductive growth, resulting in abrupt economic and agricultural losses worldwide ( Sade et al, 2018 ; Hafeez et al, 2021 ; Li et al, 2021 ). Salt and drought stress factors are the chief abiotic stress factors affecting geographical plant distribution, threatening food security, and limiting crop production ( Muhammad Ahmad et al, 2021 ).…”