Genome-wide characterization and stress-responsive expression profiling of MCM genes in Brassica oleracea and Brassica rapa

Shanmugam, Ashokraj; Robin, Arif Hasan Khan; Thamilarasan, Senthil Kumar; Vijayakumar, Harshavardhanan; Natarajan, Sathishkumar; Kim, Hoy‐Taek; Park, Jong-In; Nou, Ill–Sup

doi:10.1007/s12374-017-0105-0

Cited by 4 publications

(3 citation statements)

References 78 publications

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“…The ability of plant cells to undergo transformation and regeneration is associated with cell cycle activity [ 61 ], while the mini-chromosome maintenance protein [MCM (2-7)] complex is associated with helicase activity for replication fork formation during DNA replication [ 62 ]. So the MCM2-7 plays an essential role in the plant cell cycle.…”

Section: Discussionmentioning

confidence: 99%

Unveiling Molecular Mechanisms of Nitric Oxide-Induced Low-Temperature Tolerance in Cucumber by Transcriptome Profiling

Kong

Bian

et al. 2022

IJMS

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Cucumber (Cucumis sativus L.) is one of the most popular cultivated vegetable crops but it is intrinsically sensitive to cold stress due to its thermophilic nature. To explore the molecular mechanism of plant response to low temperature (LT) and the mitigation effect of exogenous nitric oxide (NO) on LT stress in cucumber, transcriptome changes in cucumber leaves were compared. The results showed that LT stress regulated the transcript level of genes related to the cell cycle, photosynthesis, flavonoid accumulation, lignin synthesis, active gibberellin (GA), phenylalanine metabolism, phytohormone ethylene and salicylic acid (SA) signaling in cucumber seedlings. Exogenous NO improved the LT tolerance of cucumber as reflected by increased maximum photochemical efficiency (Fv/Fm) and decreased chilling damage index (CI), electrolyte leakage and malondialdehyde (MDA) content, and altered transcript levels of genes related to phenylalanine metabolism, lignin synthesis, plant hormone (SA and ethylene) signal transduction, and cell cycle. In addition, we found four differentially expressed transcription factors (MYB63, WRKY21, HD-ZIP, and b-ZIP) and their target genes such as the light-harvesting complex I chlorophyll a/b binding protein 1 gene (LHCA1), light-harvesting complex II chlorophyll a/b binding protein 1, 3, and 5 genes (LHCB1, LHCB3, and LHCB5), chalcone synthase gene (CSH), ethylene-insensitive protein 3 gene (EIN3), peroxidase, phenylalanine ammonia-lyase gene (PAL), DNA replication licensing factor gene (MCM5 and MCM6), gibberellin 3 beta-dioxygenase gene (GA3ox), and regulatory protein gene (NPRI), which are potentially associated with plant responses to NO and LT stress. Notably, HD-ZIP and b-ZIP specifically responded to exogenous NO under LT stress. Taken together, these results demonstrate that cucumber seedlings respond to LT stress and exogenous NO by modulating the transcription of some key transcription factors and their downstream genes, thereby regulating photosynthesis, lignin synthesis, plant hormone signal transduction, phenylalanine metabolism, cell cycle, and GA synthesis. Our study unveiled potential molecular mechanisms of plant response to LT stress and indicated the possibility of NO application in cucumber production under LT stress, particularly in winter and early spring.

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Section: Discussionmentioning

confidence: 99%

Unveiling Molecular Mechanisms of Nitric Oxide-Induced Low-Temperature Tolerance in Cucumber by Transcriptome Profiling

Kong

Bian

et al. 2022

IJMS

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“…However, one study in Drosophila observed that a mutation in REC, an orthologue of MCM8, reduced crossover frequency by 95% in Drosophila (Blanton et al 2005). MCM8 has been observed to play a role in DNA replication, especially in the maintenance and progression of replication fork (Gozuacik et al 2003;Maiorano et al 2005) as well as functioning in the suppression of replication stress in B. rapa and B. oleracea species (Shanmugam et al 2017). Serra et al, (2018) suggested that elevated levels of meiotic crossover in HEI10 and recq4a/recq4b may be associated with significantly reduced seed-set in Arabidopsis.…”

Section: Discussionmentioning

confidence: 99%

Genetic factors inherited from both diploid parents interact to affect genome stability and fertility in resynthesized allotetraploid B. napus

Schierholt

et al. 2021

Preprint

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The genetic diversity in Brassica napus (canola, rapeseed) is limited because of few hybridization events and selection for important oil quality traits. One possible means of overcoming this challenge is through resynthesizing B. napus hybrids by crossing the diploid parents in order to broaden its gene pool. However, resynthesized B. napus lines are often unstable and infertile, unlike B. napus cultivars. Meiotic stability in established B. napus may have arisen through allele inheritance from the progenitor species. We tested this hypothesis by characterizing 41 resynthesized B. napus lines produced by crosses between eight B. rapa and eight B. oleracea lines for copy number variation resulting from non-homologous recombination events, allele inheritance, and fertility. We resequenced eight B. rapa and five B. oleracea parent accessions, and analyzed the allelic variation in a list of meiosis gene homologs. SNP genotyping was performed using the Illumina Infinium Brassica 60K array for three individuals per line. Self-pollinated seed-set and genome stability (number of copy number variants) were significantly affected by the interaction between both B. rapa and B. oleracea parental genotypes. We identified two putative meiosis gene candidates ( HEI10 and MCM8 ) which were significantly associated with meiosis and/or fertility as well as genome stability. Our results support the hypothesis that allelic variants inherited from parental genotypes affect genome stability and fertility in resynthesized rapeseed.

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“…Findings from Dang et al [ 46 ] indicate that MCM proteins may interact with proteins that are related to stress tolerance, and/or are involved in transcriptional regulation of stress response genes through their function as helicases. Recently, it was demonstrated that different MCM family genes were up-regulated in B. oleracea and B. rapa in response to cold and salt stress, suggesting some degree of species-specific response [ 65 ]. These previous analyses have suggested that subunits of the MCM complex are not changing in concert in response to stress, and that perhaps different subunits of the MCM complex can respond independently.…”

Section: Discussionmentioning

confidence: 99%

Physiological and transcriptomic responses in the seed coat of field-grown soybean (Glycine max L. Merr.) to abiotic stress

2017

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BackgroundUnderstanding how intensification of abiotic stress due to global climate change affects crop yields is important for continued agricultural productivity. Coupling genomic technologies with physiological crop responses in a dynamic field environment is an effective approach to dissect the mechanisms underpinning crop responses to abiotic stress. Soybean (Glycine max L. Merr. cv. Pioneer 93B15) was grown in natural production environments with projected changes to environmental conditions predicted for the end of the century, including decreased precipitation, increased tropospheric ozone concentrations ([O3]), or increased temperature.ResultsAll three environmental stresses significantly decreased leaf-level photosynthesis and stomatal conductance, leading to significant losses in seed yield. This was driven by a significant decrease in the number of pods per node for all abiotic stress treatments. To understand the underlying transcriptomic response involved in the yield response to environmental stress, RNA-Sequencing analysis was performed on the soybean seed coat, a tissue that plays an essential role in regulating carbon and nitrogen transport to developing seeds. Gene expression analysis revealed 49, 148 and 1,576 differentially expressed genes in the soybean seed coat in response to drought, elevated [O3] and elevated temperature, respectively.ConclusionsElevated [O3] and drought did not elicit substantive transcriptional changes in the soybean seed coat. However, this may be due to the timing of sampling and does not preclude impacts of those stresses on different tissues or different stages in seed coat development. Expression of genes involved in DNA replication and metabolic processes were enriched in the seed coat under high temperate stress, suggesting that the timing of events that are important for cell division and proper seed development were altered in a stressful growth environment.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1188-y) contains supplementary material, which is available to authorized users.

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Genome-wide characterization and stress-responsive expression profiling of MCM genes in Brassica oleracea and Brassica rapa

Cited by 4 publications

References 78 publications

Unveiling Molecular Mechanisms of Nitric Oxide-Induced Low-Temperature Tolerance in Cucumber by Transcriptome Profiling

Unveiling Molecular Mechanisms of Nitric Oxide-Induced Low-Temperature Tolerance in Cucumber by Transcriptome Profiling

Genetic factors inherited from both diploid parents interact to affect genome stability and fertility in resynthesized allotetraploid B. napus

Physiological and transcriptomic responses in the seed coat of field-grown soybean (Glycine max L. Merr.) to abiotic stress

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