Genome-wide association study reveals novel loci and a candidate gene for resistance to frogeye leaf spot (Cercospora sojina) in soybean

Genetic mapping of the Rcs2 locus in soybean cultivar Kent for resistance to frogeye leaf spot

“…In the recent GWAS, we reported GSM990 as the most significant marker for a genomic region on Chr 11 (McDonald, Buck, Song, et al., 2023). In the Rcs2 mapping presented here, GSM990 is a significant marker, but GSM783 was the most significant marker in the RIL population.…”

“…This is the first report of a genetic locus for Rcs2 resistance. Previously, we reported a major locus for FLS resistance at the same position on Chr 11 using a GWAS (McDonald, Buck, Song, et al, 2023), and there are no other reports of FLS resistance loci on Chr 11. In that study, 50 accessions, including several known sources of FLS resistance, carried the favorable allele at the Chr 11 locus for the most significant marker.…”

“…The genetic location of the Rcs2 locus in cultivar Kent has not been identified. In our genome‐wide association study (GWAS), a major resistance locus spanning 147 kb on Chr 11 was identified, and Kent, along with resistant cultivars Lincoln and Lee, carried the favorable marker allele as indicated by the single nucleotide polymorphism (SNP) marker GSM990 that was strongly associated with resistance to FLS (McDonald, Buck, Song, et al., 2023). Given this information, the goals of this study were to locate and confirm the genomic position of the Rcs2 locus for FLS resistance in Kent and to develop markers to select for resistance.…”

Deciphering the genetic architecture of resistance to Corynespora cassiicola in soybean (Glycine max L.) by integrating genome-wide association mapping and RNA-Seq analysis

“…Flagellin-sensitive 2 (FLS2), a LRR-RLK gene binds bacterial flagellin and initiates pathogen-associated molecular patterns (PAMPs)-triggered immunity ( Chinchilla et al., 2007 ). LRR-RLK genes have also been implicated in resistance to various pathogens, such as Cercospora sojina ( McDonald et al., 2023 ) and Phytophthora sojae ( Wang et al., 2021 ) in soybean, Blumeria graminis in wheat ( Hu et al., 2018 ), Hyaloperonospora arabidopsidis in Arabidopsis ( Hok et al., 2011 ) and Fusarium graminearum in barley and wheat ( Thapa et al., 2018 ). The RPS2 gene belongs to a LRR Class of disease resistance that plays a vital role in conferring resistance to a broad spectrum of pathogens ( Li et al., 2019 ).…”

“…The number of recombination events limit QTL mapping, whereas the association panel has accumulated recombination events over several generations, resulting in improved mapping resolution. In soybean, GWAS studies have successfully identified genes associated with resistance to various pathogens including Sclerotinia sclerotiorum ( Iquira et al., 2015 ; Wen et al., 2018 ), Pythium sylvaticum ( Lin et al., 2020 ), Heterodera glycines Inchinohe ( Vuong et al., 2015 ; Ravelombola et al., 2020 ), Fusarium virguliforme ( Bao et al., 2015 ; Zhang et al., 2015 ), Phytophthora sojae ( Li et al., 2016 ), Macrophomina phaseolina ( Coser et al., 2017 ), and Cercospora sojina ( McDonald et al., 2023 ).…”