Genome-wide association study identifies the virulence-associated marker in Streptococcus suis serotype 2

Guo, Genglin; Kong, Xuewei; Du, Dezhi; Wei, Dan; Yu, Yun William; Zhang, Wei

doi:10.1016/j.meegid.2021.104894

Cited by 7 publications

(1 citation statement)

References 52 publications

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“…The gene presence and absence data generated by the pan-genome analysis tool Roary were used, and the clinical conditions of the isolation source animals were used as phenotypes. In addition, a genome-wide association study tool, treeWAS, was used to analyse genes related to this phenotype [ 26 , 27 ]. The potential functions of the predicted genes were annotated by alignment using BLASTp and the Conserved Domain Database (CDD).…”

Section: Methodsmentioning

confidence: 99%

Genomic characterization of Streptococcus parasuis, a close relative of Streptococcus suis and also a potential opportunistic zoonotic pathogen

et al. 2022

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Streptococcus parasuis (S. parasuis) is a close relative of Streptococcus suis (S. suis), composed of former members of S. suis serotypes 20, 22 and 26. S. parasuis could infect pigs and cows, and recently, human infection cases have been reported, making S. parasuis a potential opportunistic zoonotic pathogen. In this study, we analysed the genomic characteristics of S. parasuis, using pan-genome analysis, and compare some phenotypic determinants such as capsular polysaccharide, integrative conjugative elements, CRISPR-Cas system and pili, and predicted the potential virulence genes by associated analysis of the clinical condition of isolated source animals and genotypes. Furthermore, to discuss the relationship with S. suis, we compared these characteristics of S. parasuis with those of S. suis. We found that the characteristics of S. parasuis are similar to those of S. suis, both of them have “open” pan-genome, their antimicrobial resistance gene profiles are similar and a srtF pilus cluster of S. suis was identified in S. parasuis genome. But S. parasuis still have its unique characteristics, two novel pilus clusters are and three different type CRISPR-Cas system were found. Therefore, this study provides novel insights into the interspecific and intraspecific genetic characteristics of S. parasuis, which can be useful for further study of this opportunistic pathogen, such as serotyping, diagnostics, vaccine development, and study of the pathogenesis mechanism.

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Section: Methodsmentioning

confidence: 99%