Genome-wide association study for resistance to the Meloidogyne javanica causing root-knot nematode in soybean

Single nucleotide polymorphism‐based haplotypes associated with Meloidogyne javanica resistance in Brazilian soybean germplasm

“…However, only the CD 208 and BRS CONQUISTA checks were highly resistant and 45% of the soybean entries were moderately resistant. Among the accessions showing moderate levels of resistance to this nematode, there were accessions previously reported to have high levels of resistance, including cultivars Bragg, CD 208, and BRS Conquista (Matsuo et al, 2017 (Silva, Ferraz, Arias, & Abdelnoor, 2001, Alekcevetch et al, 2021Bruinsma & Antoniolli, 2015;Matsuo et al, 2017), showed high levels of susceptibility in our study. These differences in part may reflect the known intraspecific variation for response to resistance traits that is common among isolates of species such as MJ, presenting challenges in identifying resistance genes and breeding for resistance in soybean.…”

“…Among the accessions showing moderate levels of resistance to this nematode, there were accessions previously reported to have high levels of resistance, including cultivars Bragg, CD 208, and BRS Conquista (Matsuo et al., 2017). Known susceptible accessions used in other studies, including cultivars BMX MAGNA, MMX POTENCIA, BRS 133, BRS 232, BRS 243 RR, BRS 256 RR, EMBRAPA 48, IAC 5, NA 5909, NIDERA NA 59 09 RG, R7, CD 202, and CD 206 (Silva, Ferraz, Arias, & Abdelnoor, 2001, Alekcevetch et al., 2021; Bruinsma & Antoniolli, 2015; Matsuo et al., 2017), showed high levels of susceptibility in our study. These differences in part may reflect the known intraspecific variation for response to resistance traits that is common among isolates of species such as MJ, presenting challenges in identifying resistance genes and breeding for resistance in soybean.…”

“…The distribution of the SNPs in the soybean genome provided good coverage of all the chromosomes and in intergenic, genic, and CDS regions. Alekcevetch et al (2021), using 317 soybean accessions genotyped with 46,196 SNPs provided by genotyping-bysequencing (GBS), identified 10 different SNPs associated with resistance to MJ on chr 13. According to the LD analysis, almost all the SNPs were in a unique LD block comprising a narrow region of 13.1 kb that contains only one gene model (Glyma.13g194700), which codes for a protein with a leucine-rich repeat (LRR) domain.…”

“…The genus Meloidogyne is composed of more than 90 species, but M. javanica (MJ) and M. incognita are the members of this genus with the largest economic importance in worldwide soybean production (Alekcevetch et al., 2021). In soybean, nematode parasitism deserves special attention since it can cause annual losses of 10–15%, representing almost US$78 billion in losses worldwide (Lima et al., 2017).…”

Leaf Transcriptome Analysis of Broomcorn Millet Uncovers Key Genes and Pathways in Response to Sporisorium destruens

“…The LRR domain is responsible for specific pathogen recognition [47]. The resistance genes of the LRR domain have been reported in rice, wheat, and soybean [48][49][50], but not in broomcorn millet. Here, in the plant-pathogen interaction pathway, there were three annotated DEGs from the FLS2 family in the LRR domain, including one shared DEG: Longmi038613 was defined as up-regulated DEG in S0 vs. S1, whereas it was defined as down-regulated DEG in S1 vs. S2 (Supplementary Material file 9).…”