Genome-wide association studies of net form of net blotch resistance at seedling and adult plant stages in spring barley collection

Amezrou, Reda; Verma, Ramesh Pal Singh; Chao, Shiaoman; Brueggeman, Robert; Belqadi, Loubna; Arbaoui, Mustapha; Rehman, Sajid Ur; Gyawali, Sanjaya

doi:10.1007/s11032-018-0813-2

Cited by 25 publications

(31 citation statements)

References 66 publications

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“…We suggest that seedling resistance to the newly studied isolates P3.4.0 and K5.1 is conferred in Siberian barley germplasm by the previously known locus found on chromosome 1H by Grewal et al (2012) [10] and Afanasenko et al (2014) [2]. In addition, chromosome 1H is known to carry another locus at approximately 40 cM from this one, described by Amezrou et al (2018) [8] and Vatter et al [18] (Table 5).…”

Section: Discussionsupporting

confidence: 49%

“…The genomic regions associated with resistance of barley to P. teres f. teres have been found on all barley chromosomes [3,[5][6][7][8][9][10][11][12][13][14][15][16][17][18] using both linkage mapping in biparental mapping populations and association mapping (AM). Some QTLs provide resistance during whole ontogenesis, such as QRpt6 on chromosome 6H, determining both seedling and adult resistance to the net form of net blotch [5].…”

Section: Introductionmentioning

confidence: 99%

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SNPs associated with barley resistance to isolates of Pyrenophora teres f. teres

et al. 2019

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Background: Net blotch caused by Pyrenophra teres f. teres is a major foliar disease of barley. Infection can result in significant yield losses of susceptible cultivars of up to 40%. Of the two forms of net blotch (P. teres f. teres and P. teres f. maculata), P. teres f. teres (net form of net blotch) is the dominant one in Russia. The goal of the current study was to identify genomic regions associated with seedling resistance to several pathotypes of the net form of net blotch in Siberian spring barley genotypes. For this, a genome-wide association study of a Siberian barley collection, genotyped with 50 K Illumina SNP-chip, was carried out. Results: Seedling resistance of 94 spring barley cultivars and lines to four Pyrenophora teres f. teres isolates (S10.2, K5.1, P3.4.0, and A2.6.0) was investigated. According to the Tekauz rating scale, 25, 21, 14, and 14% of genotypes were highly resistant, and 19, 8, 9, and 16% of genotypes were moderate-resistant to the isolates S10.

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Section: Discussionsupporting

confidence: 49%

Section: Introductionmentioning

confidence: 99%

SNPs associated with barley resistance to isolates of Pyrenophora teres f. teres

et al. 2019

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“…The morphological and marker diversity of AM-2014 was reported by Amezrou et al (2017). The QTL mapping of NFNB resistance in the AM-2014 panel was also reported by Amezrou et al (2018) using a genome-wide association study (GWAS) approach. Of the 336 barley genotypes in AM-2014 that were used in this study, all of them had a spring growth habit and 199 were six-rowed and 137 were two-rowed barley.…”

Section: Plant Materialsmentioning

confidence: 90%

Seedling and adult stage resistance to net form of net blotch (NFNB) in spring barley and stability of adult stage resistance to NFNB in Morocco

Gyawali

Amezrou

Verma³

et al. 2020

Journal of Phytopathology

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This study was conducted to identify stable resistance to net form of net blotch (NFNB) in spring barley in Moroccan environments. Seedling resistance to NFNB was evaluated by inoculating 336 barley genotypes with two NFNB isolates LDNH04Ptt-19 and TD-10 in the greenhouse. These genotypes were evaluated for adult plant resistance to NFNB under seven environments in Morocco in 2015 and 2016. The disease severity was estimated at GS 77-87 on barley leaves using a double-digit scale. To investigate stability of resistance, 149 barley genotypes were subjected to AMMI analysis. At the seedling stage, differential responses of barley genotypes to different NFNB isolates were identified, whereas genotypes had variable stability to NFNB resistance at the adult stages. Five genotypes, AM-68, AM-95, AM-250, AM-267 and AM-322, were resistant to both NFNB isolates at the seedling stage. There were significant (p < .001) effects of genotype (G) and G × E interaction on NFNB severity for barley genotypes at the adult stage. The principal components, IPCA1 and IPCA2, accounted for 48.4% and 18.7% variation for NFNB severity, respectively. The AMMI stability values (ASVs) ranged from 0.01 to 15.5, and fifty-nine barley genotypes had stable responses (ASV ≤ 0.05) across all seven environments. Specifically, two stable genotypes, AM-187 and AM-244, had lower mean NFNB severities across all environments, suggesting a quantitative resistance in these genotypes. Divergent environmental responses of NFNB severity were measured in Sidi El Ayedi 2015 and Sidi Allal Tazi 2016, suggesting that these environments may be suitable to capture resistance to diverse pathotypes. These stable genotypes are valuable resources for introgression of both qualitative resistance and quantitative resistance to NFNB in future. K E Y W O R D SAMMI, Barley, G × E interaction, Net blotch, NFNB, Ptt, Resistance | 255 GYAWALI et AL.

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“…A total of seven conventional genome‐wide association studies (GWAS) have been performed to investigate P. teres f. teres resistance in barley (Adhikari et al , ; Amezrou et al , ; Daba et al , ; Novakazi et al , ; Richards et al , ; Rozanova et al , ; Wonneberger et al , ). Investigation of resistance to a set of diverse global isolates was performed on the Barley Core Collection (Muñoz‐Amatriaín et al , ; Richards et al , ), Nordic Barley Panel (Wonneberger et al , ), ICARDA AM‐2014 Panel (Amezrou et al , ), Ethiopian, ICARDA and NDSU Barley Panel (Daba et al , ), Siberian Barley Panel (Rozanova et al , ), Ethiopian and Eritrean Barley Collection (Adhikari et al , ) and Vavilov Research Institute Collection (Novakazi et al , ). Between 7 and 31 unique genomic loci were identified in each GWAS (Fig.…”

Section: Introductionmentioning

confidence: 99%

Research advances in the Pyrenophora teres–barley interaction

Clare

Wyatt

Brueggeman

et al. 2019

Molecular Plant Pathology

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Summary Pyrenophora teres f. teres and P. teres f. maculata are significant pathogens that cause net blotch of barley. An increased number of loci involved in P. teres resistance or susceptibility responses of barley as well as interacting P. teres virulence effector loci have recently been identified through biparental and association mapping studies of both the pathogen and host. Characterization of the resistance/susceptibility loci in the host and the interacting effector loci in the pathogen will provide a path for targeted gene validation for better‐informed release of resistant barley cultivars. This review assembles concise consensus maps for all loci published for both the host and pathogen, providing a useful resource for the community to be used in pathogen characterization and barley breeding for resistance to both forms of P. teres.

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Genome-wide association studies of net form of net blotch resistance at seedling and adult plant stages in spring barley collection

Cited by 25 publications

References 66 publications

SNPs associated with barley resistance to isolates of Pyrenophora teres f. teres

SNPs associated with barley resistance to isolates of Pyrenophora teres f. teres

Seedling and adult stage resistance to net form of net blotch (NFNB) in spring barley and stability of adult stage resistance to NFNB in Morocco

Research advances in the Pyrenophora teres–barley interaction

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