Genome-Wide Association Mapping of Malting Quality Traits in Relevant Barley Germplasm in Uruguay

Castro, Ariel J.; Cammarota, Lorena; Gómez, Blanca Estela Reyes; Gutiérrez, Lucı́a; Hayes, Patrick; Locatelli, Andrés; Motta, L. J. M.; Pieroni, Sergio

doi:10.1007/978-94-007-4682-4_3

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Whilst neither of these studies specifically identified structural diastase genes, a number of genes with a putative role in carbohydrate metabolism more generally were associated with variation in DP. Over recent years, high density SNP (single-nucleotide polymorphism) genotyping has allowed the use of genome-wide association scans (GWAS) to survey genetic variation influencing malting quality characteristics in diverse collections (Castro et al, 2013 ). More recently still advances in genomic technology have provided tools for the generation of high density, population specific markers and genotype data.…”

Section: Introductionmentioning

confidence: 99%

Association Mapping of Diastatic Power in UK Winter and Spring Barley by Exome Sequencing of Phenotypically Contrasting Variety Sets

et al. 2017

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Diastatic Power (DP) is an important quality trait for malt used in adjunct brewing and distilling. Substantial genetic variation for DP exists within UK elite barley cultivars, but breeding progress has been slow due to the limited demand, compared to the overall barley market, and difficulties in assessing DP. Estimates of DP (taken from recommended and national list trials between 1994 and 2012) from a collection of UK elite winter and spring varieties were used to identify contrasting sets of high and low DP varieties. DNA samples were pooled within sets and exome capture sequencing performed. Allele frequency estimates of Single Nucleotide Polymorphisms (SNPs) identified from the sequencing were used to identify genomic locations associated with differences in DP. Individual genotypes were generated from a set of custom KASP assays, both within sets and in a wider germplasm collection, to validate allele frequency estimates and marker associations with DP. QTL identified regions previously linked to variation in DP as well as novel associations. QTL colocalised with a number of genes annotated as having a diastase related function. Results indicate that winter barley is more genetically diverse for genes influencing DP. The marker assays produced by this work represent a resource that is available for immediate use by barley breeders in the production of new high DP varieties.

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Section: Introductionmentioning

confidence: 99%

Association Mapping of Diastatic Power in UK Winter and Spring Barley by Exome Sequencing of Phenotypically Contrasting Variety Sets

et al. 2017

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“…QTLs or genes controlling malting traits were identified on all seven barley chromosomes, but most were identified on chromosomes 1H, 4H, 5H, and 7H (Schmalenbach, Pillen, 2009;Wang et al, 2015). Many studies investigating QTLs related to malting quality were based on genotyping data obtained using various molecular markers (Han et al, 1997;Mather et al, 1997;Coventry et al, 2003;Panozzo et al, 2007;Rae et al, 2007;Schmalenbach, Pillen, 2009;Szűcs et al, 2009;Castro et al, 2013). In addition, the database of barley markers has significantly expanded with the development of methods for detecting SNPs (single-nucleotide polymorphisms) using Illumina GoldenGate technology, which provided access to thousands of alleles and led to the creation of a high-density consensus genetic map of barley containing 2943 SNP loci (Close et al, 2009).…”

Section: Genetic Control Of Barley Malting Characteristicsmentioning

confidence: 99%

Problems and possibilities of studying malting quality in barley using molecular genetic approaches

Трубачеева

Першина

2021

Vestn. VOGiS

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About one-third of the world’s barley crop is used for malt production to meet the needs of the brewing industry. In this regard, the study of the genetic basis of malting quality traits and the breeding of malting barley varieties that are adaptive to their growing conditions are relevant throughout the world, particularly in the Russian Federation, where the cultivation and use of foreign malting varieties of barley prevails. The main parameters of malting quality (artificially germinated and dried barley grains) are malt extract, diastatic power, Kolbach index, viscosity, grain protein, wort β-glucan, free amino nitrogen, and soluble protein content. Most of these components are under the control of quantitative trait loci (QTLs) and are affected by environmental conditions, which complicates their study and precise localization. In addition, the phenotypic assessment of malting quality traits requires elaborate, expensive phenotypic analyses. Currently, there are more than 200 QTLs associated with malting parameters, which were identified using biparental mapping populations. Molecular markers are widely used both for mapping QTL loci responsible for malting quality traits and for performing marker-assisted selection (MAS), which, in combination with conventional breeding, makes it possible to create effective strategies aimed at accelerating the process of obtaining new promising genotypes. Nevertheless, the MAS of malting quality traits faces a series of difficulties, such as the low accuracy of localization of QTLs, their ineffectiveness when transferred to another genetic background, and linkage with undesirable traits, which makes it necessary to validate QTLs and the molecular markers linked to them. This review presents the results of studies that used MAS to improve the malting quality of barley, and it also considers studies that searched for associations between genotype and phenotype, carried out using GWAS (genome-wide association study) approaches based on the latest achievements of high-throughput genotyping (diversity array technology (DArT) and single-nucleotide polymorphism markers (SNPs)).

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Phenotyping variability and detecting informative SSR and EST-SSR markers for malt and straw quality characteristics in a diverse panel of wild barley (H. spontaneum) under rainfed and irrigated conditions

Shirvani,

Mehrabi,

Farshadfar

et al. 2024

Genet Resour Crop Evol

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Genome-Wide Association Mapping of Malting Quality Traits in Relevant Barley Germplasm in Uruguay

Cited by 4 publications

References 18 publications

Association Mapping of Diastatic Power in UK Winter and Spring Barley by Exome Sequencing of Phenotypically Contrasting Variety Sets

Association Mapping of Diastatic Power in UK Winter and Spring Barley by Exome Sequencing of Phenotypically Contrasting Variety Sets

Problems and possibilities of studying malting quality in barley using molecular genetic approaches

Phenotyping variability and detecting informative SSR and EST-SSR markers for malt and straw quality characteristics in a diverse panel of wild barley (H. spontaneum) under rainfed and irrigated conditions

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