Genome-Wide Association Analysis for Phosphorus Use Efficiency Traits in Mungbean (Vigna radiata L. Wilczek) Using Genotyping by Sequencing Approach

Abstract

Mungbean (Vigna radiata L. Wilczek) is an annual grain legume crop affected by low availability of phosphorus. Phosphorus deficiency mainly affects the growth and development of plants along with changes in root morphology and increase in rootto-shoot ratio. Deciphering the genetic basis of phosphorus use efficiency (PUE) traits can benefit our understanding of mungbean tolerance to low-phosphorus condition. To address this issue, 144 diverse mungbean genotypes were evaluated for 12 PUE traits under hydroponics with optimum-and low-phosphorus levels. The broad sense heritability of traits ranged from 0.63 to 0.92 and 0.58 to 0.92 under optimum-and low-phosphorus conditions, respectively. This study, reports for the first time such a large number of genome wide Single nucleotide polymorphisms (SNPs) (76,160) in mungbean. Further, genome wide association study was conducted using 55,634 SNPs obtained by genotyping-by-sequencing method. The results indicated that total 136 SNPs shared by both GLM and MLM models were associated with tested PUE traits under different phosphorus regimes. We have identified SNPs with highest p value (-log 10 (p)) for some traits like, TLA and RDW with p value (-log 10 (p)) of more than 6.0 at LP/OP and OP condition. We have identified nine SNPs (three for TLA and six for RDW trait) which was found to be present in chromosomes 8, 4, and 7. One SNP present in Vradi07g06230 gene contains zinc finger CCCH domain. In total, 71 protein coding genes were identified, of which 13 genes were found to be putative candidate genes controlling PUE by regulating nutrient uptake and root architectural development pathways in mungbean. Moreover, we identified three

Novel Genes and Genetic Loci Associated With Root Morphological Traits, Phosphorus-Acquisition Efficiency and Phosphorus-Use Efficiency in Chickpea

“…Further, this SNP locus was associated with total shoot P content and shoot DW in different models (Supplementary Table 1 and Figure 2). Such shared associations were also reported using different models in mungbean PUE (Reddy et al, 2020). A recent study reported that low expression of selected fasciclin-like arabinogalactan protein genes led to kernel abortion in maize (Zea mays) and Arabidopsis thaliana seeds (Cagnola et al, 2018).…”

WITHDRAWN: Effectiveness of the Classification for the Phosphorus Use Efficiency Traits at the Seedling Stage in Lentil

“…Lentil genotypes differed for their reaction to the P de cit mediums. Various crops showed variation for their reaction to P, wheat [47][48], oats, barley, triticale and rye [49], maize [50], cotton [51][52], groundnut [53], brassica [54], soybean [55], faba bean [56], common bean [57], lentil [58], and the mungbean [59]. Many studies routinely used targeting to few measures of P-use e ciency [60] and other studies targeted speci c root architectural traits [61] and while another vital trait root hair formation was also considered [62].…”

Cross Tolerance to Drought and low Phosphorus Stress in Mungbean is Regulated by Improved Antioxidant Capacity, Biological N2-Fixation, and Differential Transcript Accumulation

“…Tissue P concentration, biomass accumulation, and P uptake were the most sensitive P-de ciency traits to stress, as reported in mungbean (Pandey et al, 2014) and soybean . Wide genotypic variation in phosphorus use e ciency (PUE) has been reported in mungbean (Pandey et al 2013;Meena et al 2020;Reddy et al 2020), indicating that Pe cient genotypes uptake more P to shoots than roots. This suggests that P translocation from roots to shoots is an adaptive strategy to increase PUE (Krishnappa et al 2011;Krishnappa and Hussain 2014).…”