Genome-wide annotation of cuticular proteins in the oriental fruit fly ( Bactrocera dorsalis ), changes during pupariation and expression analysis of CPAP3 protein genes in response to environmental stresses

Chen, Er-Hu; Hou, Qiu‐Li; Dou, Wei; Wei, Dandan; Yue, Yong; Yang, Ruining; Yang, Pei-Shan; Yu, Shuai-Feng; Schutter, Kristof De; Smagghe, Guy; Wang, Jinjun

doi:10.1016/j.ibmb.2018.04.009

Cited by 38 publications

(46 citation statements)

References 81 publications

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“…In insects, the structure of the cuticle is determined by cuticular proteins, which play roles in many physiological processes, such as protection against dehydration, insecticide penetration, pathogens and physical injury 46 . Therefore, CPs could be used as potential targets for the management of insect pests 47 . Some genes encoding cuticular binding proteins and cuticular proteins analogous to peritrophins (CPAPs) were differentially expressed in our data set, with higher expression being observed in larvae and males than in females (Fig.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and gene expression analysis of three developmental stages of the coffee berry borer, Hypothenemus hampei

Noriega

Arias

Barbosa

et al. 2019

Sci Rep

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Coffee production is a global industry valued at approximately 173 billion US dollars. One of the main challenges facing coffee production is the management of the coffee berry borer (CBB), Hypothenemus hampei , which is considered the primary arthropod pest of coffee worldwide. Current control strategies are inefficient for CBB management. Although biotechnological alternatives, including RNA interference (RNAi), have been proposed in recent years to control insect pests, characterizing the genetics of the target pest is essential for the successful application of these emerging technologies. In this study, we employed RNA-seq to obtain the transcriptome of three developmental stages of the CBB (larva, female and male) to increase our understanding of the CBB life cycle in relation to molecular features. The CBB transcriptome was sequenced using Illumina Hiseq and assembled de novo . Differential gene expression analysis was performed across the developmental stages. The final assembly produced 29,434 unigenes, of which 4,664 transcripts were differentially expressed. Genes linked to crucial physiological functions, such as digestion and detoxification, were determined to be tightly regulated between the reproductive and nonreproductive stages of CBB. The data obtained in this study help to elucidate the critical roles that several genes play as regulatory elements in CBB development.

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Section: Discussionmentioning

confidence: 99%

Transcriptome and gene expression analysis of three developmental stages of the coffee berry borer, Hypothenemus hampei

Noriega

Arias

Barbosa

et al. 2019

Sci Rep

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“…However, the CPAP3 family genes were the exceptions, and their number and sequence structures were conserved, which indicates their essential roles in insect survival and fitness (Gallot et al ., 2010; Chen et al ., 2018a). Therefore, we selected the CPAP3 family genes to carry out further functional analysis, and our previous research showed that these genes might help adult B. dorsalis flies cope with thermal, pesticide, and dehydration stresses (Chen et al ., 2018a). In the current study, we mainly focused on the roles of CPAP3 genes in the physical development of B. dorsalis .…”

Section: Discussionmentioning

confidence: 99%

“…An Mx3 000P thermal cycler (Stratagene, La Jolla, CA, USA) was used for qRT‐PCR under the following conditions: 2 min denaturation at 95 °C, 95 °C for 15 s of 40 cycles and 60 °C for 30 s. Simultaneously, a melting curve analysis was conducted during reactions from 60 to 95 °C to further ensure product specificity and consistency. Previous studies have shown that α‐Tubulin (GenBank accession number: GU269902) owned excellent stability as an internal reference gene in B. dorsalis , and the relative expression was analyzed by the 2 −ΔΔCt method (Shen et al ., 2010; Chen et al ., 2018a; Chen et al ., 2018b; Chen et al ., 2018c). In this study, three biological with two technical repeats were carried out for all qPCR experiments, and those data were analyzed by SPSS 16.0 for Windows (SPSS Inc., Chicago, IL, USA) and presented as mean relative expression level ± SE.…”

Section: Methodsmentioning

confidence: 99%

Knockdown of specific cuticular proteins analogous to peritrophin 3 genes disrupt larval and ovarian development in Bactrocera dorsalis (Diptera: Tephritidae)

et al. 2020

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Cuticular proteins (CPs) are critical components of the insect cuticle and play important roles in maintaining normal insect development and defense against various environmental stresses. The oriental fruit fly (Bactrocera dorsalis) is one of the most destructive pests worldwide, and its eight CPs analogous to peritrophin 3 (BdCPAP3) family genes have been identified in our previous study. In the present study, we further explored the possible roles of CPAP3 genes in B. dorsalis development. Each sequence of BdCPAP3 genes contained three conserved ChtBD2 (chitin‐binding) domains. Spatial and temporal expression patterns revealed that the four BdCPAP3 genes (BdCPAP3‐A1, B, E, and E2) might play important roles in larval pupariation of B. dorsalis. Moreover, treatment with a juvenile hormone analog (methoprene) significantly restricted expression of these four CPAP3 genes, whereas treatment with 20‐hydroxy‐ecdysone induced expression. The RNA interference (RNAi) results revealed that down‐regulated CPAP3 genes led to significant delay of pupariation, and injection of dsBdCPAP3‐E into 5‐d‐old B. dorsalis larvae caused approximately 40% mortality. Interestingly, we also confirmed that BdCPAP3‐D2 was involved in B. dorsalis ovarian development. This study showed that some specific CPAP3 genes had crucial roles in B. dorsalis development, and these CP genes could be used as potential targets to control this pest via RNAi.

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“…Knock-down of the other CPAP3 caused severe molting and development defects. This group is associated with insect adaptation to severe environmental conditions (Chen et al, 2018). The protection afforded by the host cuticle during the developmental stages of M. mediator might decrease the necessity of these CPs.…”

Section: Discussionmentioning

confidence: 99%

Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator

Volovych

Zhe

et al. 2019

Insect Science

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Recently, parasitoid wasp species Microplitis mediator has evoked increasing research attention due to its possible use in the control of Lepidoptera insects. Because insect development involves changes in cuticle composition, identification and expression analysis of M. mediator cuticular proteins may clarify the mechanisms involved in parasite development processes. We found 70 cuticular proteins from the M. mediator transcriptome and divided them into seven distinct families. Expression profiling indicated that most of these cuticular protein genes have expression peaks specific for one particular developmental stage of M. mediator. Eggs and pupae have the highest number of transcriptionally active cuticular protein genes (47 and 52 respectively). Only 12 of these genes maintained high expression activity during late larval development. Functional analysis of two larval proteins, MmCPR3 and MmCPR14, suggested their important role in the proper organization of the cuticle layers of larvae. During M. mediator larval development, normal cuticle formation can be supported by a limited number of cuticular proteins.

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Genome-wide annotation of cuticular proteins in the oriental fruit fly ( Bactrocera dorsalis ), changes during pupariation and expression analysis of CPAP3 protein genes in response to environmental stresses

Cited by 38 publications

References 81 publications

Transcriptome and gene expression analysis of three developmental stages of the coffee berry borer, Hypothenemus hampei

Transcriptome and gene expression analysis of three developmental stages of the coffee berry borer, Hypothenemus hampei

Knockdown of specific cuticular proteins analogous to peritrophin 3 genes disrupt larval and ovarian development in Bactrocera dorsalis (Diptera: Tephritidae)

Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator

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