Genome-wide analysis of transcriptional changes in the thoracic muscle of the migratory locust, Locusta migratoria, exposed to hypobaric hypoxia

Zhao, Degang; Zhang, Zhen Yu; Harrison, Jon F.; Kang, Le

doi:10.1016/j.jinsphys.2012.08.006

Cited by 15 publications

(11 citation statements)

References 78 publications

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“…In comparison, alpha-glucosidase and beta-fructofuranosidase were down-regulated in high-altitude larvae, indicating a decrease in carbohydrate utilization. This result was consistent with the hypothesis that lipids serve as an optimal metabolic fuel source in locusts, deer mice, plateau zokor, and plateau pikas at high altitudes Cheviron et al, 2012;Zhao et al, 2012;Zhao et al, 2013). This phenomenon may exist because lipids make up more than 80% of the total energy reserves in animals and because the energy density of lipids is greater than that of carbohydrates (Weber, 2011), making them the preferred fuel source at high altitudes (McClelland et al, 1994;McClelland, 2004).…”

Section: Accepted Manuscriptsupporting

confidence: 88%

De novo transcriptome characterization of the ghost moth, Thitarodes pui, and elevation-based differences in the gene expression of its larvae

Sun

Guo

et al. 2015

Gene

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Section: Accepted Manuscriptsupporting

confidence: 88%

De novo transcriptome characterization of the ghost moth, Thitarodes pui, and elevation-based differences in the gene expression of its larvae

Sun

Guo

et al. 2015

Gene

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“…We identified 324 differentially expressed genes (DEGs) up-regulated in TP locusts and 555 DEGs up-regulated in NP locusts (Additional file 1). Of the 879 genes, only 24 were among the 235 previously identified target genes of hypoxia-inducible factor [11]. Based on GO, KEGG and NCBI annotations, DEGs were further classified into 14 functional categories (Figure 3, Additional file 1), which include a variety of basic functions, e.g.…”

Section: Resultsmentioning

confidence: 99%

“…Total RNA was extracted following the protocol of the RNeasy Mini Kit (Qiagen). Then, RNA was reversely transcribed into cDNA, labeled with Cy3 and Cy5, and finally hybridized to locust microarrays as previously described [11-14]. Microarrays were scanned by using a GenePix 4000B microarray scanner and image analysis was performed by using GenePix Pro 6.0 (Axon Instruments).…”

Section: Methodsmentioning

confidence: 99%

“…TP locusts likely expanded into Tibet 34,000 and 40,000 years ago and have since inhabited the Tibetan Plateau [6]. Although the partial oxygen pressure ( p O 2 ) in Tibet (~13 kPa) is above the critical oxygen pressure of locusts (~3 kPa) [9,10], the Tibetan p O 2 still exerts selective pressures on locusts [11]. Long-term habitation to the Tibetan Plateau probably has adapted TP locusts to the chronic low-oxygen atmosphere, conferring on them superior tolerance of hypoxia.…”

Section: Introductionmentioning

confidence: 99%

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Efficient utilization of aerobic metabolism helps Tibetan locusts conquer hypoxia

et al. 2013

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BackgroundResponses to hypoxia have been investigated in many species; however, comparative studies between conspecific geographical populations at different altitudes are rare, especially for invertebrates. The migratory locust, Locusta migratoria, is widely distributed around the world, including on the high-altitude Tibetan Plateau (TP) and the low-altitude North China Plain (NP). TP locusts have inhabited Tibetan Plateau for over 34,000 years and thus probably have evolved superior capacity to cope with hypoxia.ResultsHere we compared the hypoxic responses of TP and NP locusts from morphological, behavioral, and physiological perspectives. We found that TP locusts were more tolerant of extreme hypoxia than NP locusts. To evaluate why TP locusts respond to extreme hypoxia differently from NP locusts, we subjected them to extreme hypoxia and compared their transcriptional responses. We found that the aerobic metabolism was less affected in TP locusts than in NP locusts. RNAi disruption of PDHE1β, an entry gene from glycolysis to TCA cycle, increased the ratio of stupor in TP locusts and decreased the ATP content of TP locusts in hypoxia, confirming that aerobic metabolism is critical for TP locusts to maintain activity in hypoxia.ConclusionsOur results indicate that TP and NP locusts have undergone divergence in hypoxia tolerance. These findings also indicate that insects can adapt to hypoxic pressure by modulating basic metabolic processes.

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“…This metabolic reconfiguration increases the flux of NADPH generation, therefore providing sufficient reducing power for combating oxidative challenges [57, 58]. In the migratory locust, Locusta migratoria , a genome-wide transcription analysis revealed that under hypobaric hypoxia-induced oxidative stress, glycolysis was suppressed, and PPP was enhanced [59]. In a Drosophila model, a knockdown of the gene encoding ribose-5-phosphate isomerase (RPI) increases glucose-6-phosphate dehydrogenase (G6PD) activity, which results in an elevated level of both NADPH and GSH.…”

Section: Oxidative Stress-induced Metabolic Reconfigurationmentioning

confidence: 99%

The impact of metagenomic interplay on the mosquito redox homeostasis

Champion

2017

Free Radical Biology and Medicine

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Mosquitoes are exposed to oxidative challenges throughout their life cycle. The primary challenge comes from a blood meal. The blood digestion turns the midgut into an oxidative environment, which imposes pressure not only on mosquito fecundity and other physiological traits but also on the microbiota in the midgut. During evolution, mosquitoes have developed numerous oxidative defense mechanisms to maintain redox homeostasis in the midgut. In addition to antioxidants, SOD, catalase, and glutathione system, sufficient supply of the reducing agent, NADPH, is vital for a successful defense against oxidative stress. Increasing evidence indicates that in response to oxidative stress, cells reconfigure metabolic pathways to increase the generation of NADPH through NADP-reducing networks including the pentose phosphate pathway and others. The microbial homeostasis is critical for the functional contributions to various host phenotypes. The symbiotic microbiota is regulated largely by the Duox-ROS pathway in Drosophila. In mosquitoes, Duox-ROS pathway, heme-mediated signaling, antimicrobial peptide production and C-type lectins work in concert to maintain the dynamic microbial community in the midgut. Microbial mechanisms against oxidative stress in this context are not well understood. Emerging evidence that microbial metabolites trigger host oxidative response warrants further study on the metagenomic interplay in an oxidative environment like mosquito gut ecosystem. Besides the classical Drosophila model, hematophagous insects like mosquitoes provide an alternative model system to study redox homeostasis in a symbiotic metagenomic context.

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Genome-wide analysis of transcriptional changes in the thoracic muscle of the migratory locust, Locusta migratoria, exposed to hypobaric hypoxia

Cited by 15 publications

References 78 publications

De novo transcriptome characterization of the ghost moth, Thitarodes pui, and elevation-based differences in the gene expression of its larvae

De novo transcriptome characterization of the ghost moth, Thitarodes pui, and elevation-based differences in the gene expression of its larvae

Efficient utilization of aerobic metabolism helps Tibetan locusts conquer hypoxia

The impact of metagenomic interplay on the mosquito redox homeostasis

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