Genome-wide analysis of the Tritipyrum WRKY gene family and the response of TtWRKY256 in salt-tolerance

Li, Kuiyin; Liu, Xiaojuan; He, Fang; Chen, Songshu; Zhou, Guangyi; Wang, Yuhai; Li, Lu Hua; Zhang, Suqin; Ren, Mingjian; Yuan, Yuanyuan

doi:10.3389/fpls.2022.1042078

Cited by 5 publications

(2 citation statements)

References 49 publications

(60 reference statements)

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“…The 2 −ΔΔCt technique was utilized to ascertain the levels of expression. The real-time polymerase chain reaction (PCR) settings and the determination of relative gene expression were performed according to the methods previously outlined in reference [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Genome-wide analysis of the Tritipyrum NAC gene family and the response of TtNAC477 in salt tolerance

Liu,

Zhou,

Chen

et al. 2024

BMC Plant Biol

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NAC transcription factors are widely distributed in the plant kingdom and play an important role in the response to various abiotic stresses in plant species. Tritipyrum, an octoploid derived from hybridization of Triticum aestivum (AABBDD) and Thinopyrum elongatum (EE), is an important genetic resource for integrating the desirable traits of Th. elongatum into wheat. In this study, we investigated the tissue distribution and expression of Tritipyrum NAC genes in the whole genomes of T. aestivum and Th. elongatum after obtaining their complete genome sequences. Based on phylogenetic relationships, conserved motifs, gene synthesis, evolutionary analysis, and expression patterns, we identified and characterized 732 Tritipyrum NAC genes. These genes were divided into six main groups (A, B, C, D, E, and G) based on phylogenetic relationships and evolutionary studies, with members of these groups sharing the same motif composition. The 732 TtNAC genes are widely distributed across 28 chromosomes and include 110 duplicated genes. Gene synthesis analysis indicated that the NAC gene family may have a common ancestor. Transcriptome data and quantitative polymerase chain reaction (qPCR) expression profiles showed 68 TtNAC genes to be highly expressed in response to various salt stress and recovery treatments. Tel3E01T644900 (TtNAC477) was particularly sensitive to salt stress and belongs to the same clade as the salt tolerance genes ANAC019 and ANAC055 in Arabidopsis. Pearson correlation analysis identified 751 genes that correlated positively with expression of TtNAC477, and these genes are enriched in metabolic activities, cellular processes, stimulus responses, and biological regulation. TtNAC477 was found to be highly expressed in roots, stems, and leaves in response to salt stress, as confirmed by real-time PCR. These findings suggest that TtNAC477 is associated with salt tolerance in plants and might serve as a valuable exogenous gene for enhancing salt tolerance in wheat.

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Section: Methodsmentioning

confidence: 99%

Genome-wide analysis of the Tritipyrum NAC gene family and the response of TtNAC477 in salt tolerance

Liu,

Zhou,

Chen

et al. 2024

BMC Plant Biol

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“…WRKY is one of the biggest transcription factor (TF) family in plants like Arabidopsis thaliana [ 1 ], Oryza sativa [ 2 ], Tritipyrum [ 3 ], and Isatis indigotica [ 4 ]. WRKY proteins have highly conserved structure, containing one or two WRKY domains composed of approximately 60 amino acid residues.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of WRKY transcription factors in Casuarina equisetifolia and the function analysis of CeqWRKY11 in response to NaCl/NaHCO3 stresses

Zhao,

Qi,

Liu

et al. 2024

BMC Plant Biol

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Background Casuarina equisetifolia (C. equisetifolia) is a woody species with many excellent features. It has natural resistance against drought, salt and saline-alkali stresses. WRKY transcription factors (TFs) play significant roles in plant response to abiotic stresses, therefore, molecular characterization of WRKY gene family under abiotic stresses holds great significance for improvement of forest trees through molecular biological tools. At present, WRKY TFs from C. equisetifolia have not been thoroughly studied with respect to their role in salt and saline-alkali stresses response. The current study was conducted to bridge the same knowledge gap. Results A total of 64 WRKYs were identified in C. equisetifolia and divided into three major groups i.e. group I, II and III, consisting of 10, 42 and 12 WRKY members, respectively. The WRKY members in group II were further divided into 5 subgroups according to their homology with Arabidopsis counterparts. WRKYs belonging to the same group exhibited higher similarities in gene structure and the presence of conserved motifs. Promoter analysis data showed the presence of various response elements, especially those related to hormone signaling and abiotic stresses, such as ABRE (ABA), TGACG (MeJA), W-box ((C/T) TGAC (T/C)) and TC-rich motif. Tissue specific expression data showed that CeqWRKYs were mainly expressed in root under normal growth conditions. Furthermore, most of the CeqWRKYs were up-regulated by NaCl and NaHCO3 stresses with few of WRKYs showing early responsiveness to both stresses while few others exhibiting late response. Although the expressions of CeqWRKYs were also induced by cold stress, the response was delayed compared with other stresses. Transgenic C. equisetifolia plants overexpressing CeqWRKY11 displayed lower electrolyte leakage, higher chlorophyll content, and enhanced tolerance to both stresses. The higher expression of abiotic stress related genes, especially CeqHKT1 and CeqPOD7, in overexpression lines points to the maintenance of optimum Na+/K+ ratio, and ROS scavenging as possible key molecular mechanisms underlying salt stress tolerance. Conclusions Our results show that CeqWRKYs might be key regulators of NaCl and NaHCO3 stresses response in C. equisetifolia. In addition, positive correlation of CeqWRKY11 expression with increased stress tolerance in C. equisetifolia encourages further research on other WRKY family members through functional genomic tools. The best candidates could be incorporated in other woody plant species for improving stress tolerance.

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WRKY transcription factors in plant defense

Javed,

Gao

2023

Trends in Genetics

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Genome-wide analysis of the Tritipyrum WRKY gene family and the response of TtWRKY256 in salt-tolerance

Cited by 5 publications

References 49 publications

Genome-wide analysis of the Tritipyrum NAC gene family and the response of TtNAC477 in salt tolerance

Genome-wide analysis of the Tritipyrum NAC gene family and the response of TtNAC477 in salt tolerance

Genome-wide identification of WRKY transcription factors in Casuarina equisetifolia and the function analysis of CeqWRKY11 in response to NaCl/NaHCO3 stresses

WRKY transcription factors in plant defense

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