Genome-Wide Analysis of the Temporal Genetic Changes in Streptococcus pneumoniae Isolates of Genotype ST320 and Serotype 19A from South Korea

Baek, Jin Yang; Kim, Sun Ju; Shin, Juyoun; Chung, Yeun‐Jun; Kang, Cheol-In; Chung, Doo Ryeon; Song, Jae–Hoon; Ko, Kwan Soo

doi:10.3390/microorganisms9040795

Cited by 2 publications

(1 citation statement)

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“…To clarify the genetic relationships between global serotype 19A-ST320 isolates and our samples, we downloaded the draft genome data of 885 GPSC1 isolates published by Gladstone et al ( 29 ) because all 19A-ST320 isolates collected in Japan belonged to GPSC1. Additionally, we downloaded data from public databases for 7 isolates from China ( 30 , 31 ), 25 isolates from South Korea ( 32 ), 11 isolates from Myanmar ( 33 ) and 10 isolates from Canada. When only assembled contigs were available, we generated 2 × 150-bp paired-end reads using BBmap ( https://sourceforge.net/projects/bbmap/ ).…”

Section: Methodsmentioning

confidence: 99%

Whole-Genome Analysis-Based Phylogeographic Investigation of Streptococcus pneumoniae Serotype 19A Sequence Type 320 Isolates in Japan

Nakano

Fujisawa

Chang

et al. 2022

Antimicrob Agents Chemother

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After the introduction of the seven-valent pneumococcal conjugate vaccine, the global spread of multidrug resistant serotype 19A-ST320 strains became a public health concern. In Japan, the main genotype of serotype 19A was ST3111, and the identification rate of ST320 was low. Although the isolates were sporadically detected in both adults and children, their origin remains unknown. Thus, by combining pneumococcal isolates collected in three nationwide pneumococcal surveillance studies conducted in Japan between 2008 and 2020, we analyzed 56 serotype 19A-ST320 isolates along with 931 global isolates, using whole-genome sequencing to uncover the transmission route of the globally distributed clone in Japan. The clone was frequently detected in Okinawa Prefecture, where the U.S. returned to Japan in 1972. Phylogenetic analysis demonstrated that the isolates from Japan were genetically related to those from the U.S.; therefore, the common ancestor may have originated in the U.S. In addition, Bayesian analysis suggested that the time to the most recent common ancestor of the isolates form Japan and the U.S. was approximately the 1990s to 2000, suggesting the possibility that the common ancestor could have already spread in the U.S. before the Taiwan 19F-14 isolate was first identified in a Taiwanese hospital in 1997. The phylogeographical analysis supported the transmission of the clone from the U.S. to Japan, but the analysis could be influenced by sampling bias. These results suggested the possibility that the serotype 19A-ST320 clone had already spread in the U.S. before being imported into Japan.

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