Genome‐wide analysis of the POU genes in medaka, focusing on expression in the optic tectum

Brombin, Alessandro; Grossier, Jean-Philippe; Heuzé, Aurélie; Radev, Zlatko; Bourrat, Franck; Joly, Jean‐Stéphane; Jamen, Françoise

doi:10.1002/dvdy.22727

Cited by 10 publications

(10 citation statements)

References 64 publications

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“…2) (Sanchez‐Sanchez et al, 2010). In contrast to previous data on pou2 expression in medaka, the brain continuously expresses oct4 ‐EGFP at later stages in all analyzed transgenic lines (Sanchez‐Sanchez et al, 2010; Brombin et al, 2011). A specific function of pou2 in the medaka brain has not been described, but ectopic expression of the simplet gene ( smp ) induces the expression of pou2 in additional brain regions of medaka embryos at stage 19 (Thermes et al, 2006).…”

Section: Discussioncontrasting

confidence: 95%

oct4‐EGFP reporter gene expression marks the stem cells in embryonic development and in adult gonads of transgenic medaka

Froschauer

Khatun

Sprott

et al. 2012

Molecular Reproduction Devel

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Maintenance of pluripotency in stem cells is tightly regulated among vertebrates. One of the key genes in this process is oct4, also referred to as pou5f1 in mammals and pou2 in teleosts. Pou5f1 evolved by duplication of pou2 early in the tetrapod lineage, but only monotremes and marsupials retained both genes. Either pou2 or pou5f1 was lost from the genomes of the other tetrapods that have been analyzed to date. Consequently, these two homologous genes are often designated oct4 in functional studies. In most vertebrates oct4 is expressed in pluripotent cells of the early embryo until the blastula stage, and later persist in germline stem cells until adulthood. The isolation and analysis of stem cells from embryo or adult individuals is hampered by the need for reliable markers that can identify and define the cell populations. Here, we report the faithful expression of EGFP under the control of endogenous pou2/oct4 promoters in transgenic medaka (Oryzias latipes). In vivo imaging in oct4-EGFP transgenic medaka reveals the temporal and spatial expression of pou2 in embryos and adults alike. We describe the temporal and spatial patterns of endogenous pou2 and oct4-EGFP expression in medaka with respect to germline and adult stem cells, and discuss applications of oct4-EGFP transgenic medaka in reproductive and stem cell biology.

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Section: Discussioncontrasting

confidence: 95%

oct4‐EGFP reporter gene expression marks the stem cells in embryonic development and in adult gonads of transgenic medaka

Froschauer

Khatun

Sprott

et al. 2012

Molecular Reproduction Devel

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“…3D-F). Brn1/2/4, a POU family transcription factor with neural specification roles in other organisms (Ryan and Rosenfeld, 1997;Wegner et al, 1993;Brombin et al, 2011), is also expressed by differentiated neurons within the ciliary band, where it localizes to nuclei ( Fig. 3A-F).…”

Section: Soxc and Neural Progenitorsmentioning

confidence: 99%

Neurogenesis in sea urchin embryos and the diversity of deuterostome neurogenic mechanisms

et al. 2015

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A single origin to the diverse mechanisms of metazoan neurogenesis is suggested by the involvement of common signaling components and similar classes of transcription factors. However, in many forms we lack details of where neurons arise, patterns of cell division, and specific differentiation pathway components. The sea urchin larval nervous system is composed of an apical organ, which develops from neuroepithelium and functions as a central nervous system, and peripheral neurons, which differentiate in the ciliary band and project axons to the apical organ. To reveal developmental mechanisms of neurogenesis in this basal deuterostome, we developed antibodies to SoxC, SoxB2, ELAV and Brn1/2/4 and used neurons that develop at specific locations to establish a timeline for neurogenesis. Neural progenitors express, in turn, SoxB2, SoxC, and Brn1/2/4, before projecting neurites and expressing ELAV and SynB. Using pulse-chase labeling of cells with a thymidine analog to identify cells in S-phase, we establish that neurons identified by location are in their last mitotic cycle at the time of hatching, and S-phase is coincident with expression of SoxC. The number of cells expressing SoxC and differentiating as neurons is reduced in embryos injected with antisense morpholino oligonucleotides to SoxC, SoxB2 or Six3. Injection of RNA encoding SoxC into eggs does not enhance neurogenesis. In addition, inhibition of FGF receptors (SU5402) or a morpholino to FGFR1 reduces expression of SoxC. These data indicate that there are common features of neurogenesis in deuterostomes, and that sea urchins employ developmental mechanisms that are distinct from other ambulacraria.

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“…Antisense riboprobes and paraffin sections were prepared as previously described (Brombin et al, 2011). Sequences of the DIG riboprobes used for in situ hybridisation are given in supplementary material Table S4.…”

Section: Histologymentioning

confidence: 99%

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis

et al. 2013

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Investigating neural stem cell (NSC) behaviour in vivo, which is a major area of research, requires NSC models to be developed. We carried out a multilevel characterisation of the zebrafish embryo peripheral midbrain layer (PML) and identified a unique vertebrate progenitor population. Located dorsally in the transparent embryo midbrain, these large slow-amplifying progenitors (SAPs) are accessible for long-term in vivo imaging. They form a neuroepithelial layer adjacent to the optic tectum, which has transitory fast-amplifying progenitors (FAPs) at its margin. The presence of these SAPs and FAPs in separate domains provided the opportunity to data mine the ZFIN expression pattern database for SAP markers, which are coexpressed in the retina. Most of them are involved in nucleotide synthesis, or encode nucleolar and ribosomal proteins. A mutant for the cad gene, which is strongly expressed in the PML, reveals severe midbrain defects with massive apoptosis and sustained proliferation. We discuss how fish midbrain and retina progenitors might derive from ancient sister cell types and have specific features that are not shared with other SAPs.

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Genome‐wide analysis of the POU genes in medaka, focusing on expression in the optic tectum

Cited by 10 publications

References 64 publications

oct4‐EGFP reporter gene expression marks the stem cells in embryonic development and in adult gonads of transgenic medaka

oct4‐EGFP reporter gene expression marks the stem cells in embryonic development and in adult gonads of transgenic medaka

Neurogenesis in sea urchin embryos and the diversity of deuterostome neurogenic mechanisms

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis

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