Genome‐wide analysis of the general stress response in <i>Bacillus subtilis</i>

Price, Chester W.; Fawcett, Paul; Cérémonie, Hélène; Su, Nancy; Murphy, Christian; Youngman, Philip

doi:10.1046/j.1365-2958.2001.02534.x

Cited by 251 publications

(272 citation statements)

References 91 publications

Supporting

Mentioning

263

Contrasting

Unclassified

Order By: Relevance

“…Unlike the synthesis of clpC or clpP mRNA, neither mecA nor ypbH transcription is heat shock-regulated (41)(42)(43). However, it was already observed in vivo and in vitro that MecA is also a ClpCP substrate (23,44).…”

Section: Discussionmentioning

confidence: 99%

MecA, an adaptor protein necessary for ClpC chaperone activity

Schlothauer

Mogk

Dougan

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

140

176

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

MecA, an adaptor protein necessary for ClpC chaperone activity

Schlothauer

Mogk

Dougan

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

140

176

View full text Add to dashboard Cite

show abstract

“…, and X regulons using the DBTBS database (55) and additional data from genome-wide studies (8,12,33,51,52).…”

Section: Methodsmentioning

confidence: 99%

“…Environmental and cellular stress conditions are detected by a complex set of sensor and regulatory proteins that are organized in the stressosome (43). Signal output from the stressosome activates the alternative transcription factor B , which in turn triggers the coordinated transcription of about 150 genes comprising a general stress regulon (51,52). Thirty-seven general stress proteins have an important function in cellular protection against a severe salt shock since the disruption of their structural genes causes a salt-sensitive phenotype.…”

mentioning

confidence: 99%

A Comprehensive Proteomics and Transcriptomics Analysis ofBacillus subtilisSalt Stress Adaptation

et al. 2010

View full text Add to dashboard Cite

In its natural habitats, Bacillus subtilis is exposed to changing osmolarity, necessitating adaptive stress responses. Transcriptomic and proteomic approaches can provide a picture of the dynamic changes occurring in salt-stressed B. subtilis cultures because these studies provide an unbiased view of cells coping with high salinity. We applied whole-genome microarray technology and metabolic labeling, combined with state-of-theart proteomic techniques, to provide a global and time-resolved picture of the physiological response of B. subtilis cells exposed to a severe and sudden osmotic upshift. This combined experimental approach provided quantitative data for 3,961 mRNA transcription profiles, 590 expression profiles of proteins detected in the cytosol, and 383 expression profiles of proteins detected in the membrane fraction. Our study uncovered a well-coordinated induction of gene expression subsequent to an osmotic upshift that involves large parts of the SigB, SigW, SigM, and SigX regulons. Additionally osmotic upregulation of a large number of genes that do not belong to these regulons was observed. In total, osmotic upregulation of about 500 B. subtilis genes was detected. Our data provide an unprecedented rich basis for further in-depth investigation of the physiological and genetic responses of B. subtilis to hyperosmotic stress.

show abstract

“…Until recently studies concerning the adaptation of cells against osmotic stress have been essentially oriented to genetic and physiological regulation aspects (Csonka & Hanson, 1991;von Blohn et al, 1997;Glassker et al, 1998;Kappes et al, 1996;Price et al, 2001). Recently, a transcriptome analysis of the adaptation of B. subtilis cells to high salinity showed that at least 125 genes are induced, thus revealing the complexity of this response (Steil et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, a transcriptional microarray analysis of stress responses has indicated that the ywiE gene is induced during ethanol or NaCl stress, its promoter being sigma B-dependent, as for most of the genes responding to general stress effectors (Price et al, 2001;Petersohn et al, 2001). Moreover, recently Kawai et al (2004) showed that in the B. subtilis Marburg strain, ywnE is the gene that plays the main role in CL synthesis and therefore renamed it as clsA.…”

Section: Introductionmentioning

confidence: 99%

Role of anionic phospholipids in the adaptation of Bacillus subtilis to high salinity

et al. 2006

View full text Add to dashboard Cite

The importance of the content of anionic phospholipids [cardiolipin (CL) and phosphatidylglycerol (PG)] in the osmotic adaptation and in the membrane structure of Bacillus subtilis cultures was investigated. Insertion mutations in the three putative cardiolipin synthase genes (ywiE, ywnE and ywjE) were obtained. Only the ywnE mutation resulted in a complete deficiency in cardiolipin and thus corresponds to a true clsA gene. The osmotolerance of a clsA mutant was impaired: although at NaCl concentrations lower than 1?2 M the growth curves were similar to those of its wild-type control, at 1?5 M NaCl (LBN medium) the lag period increased and the maximal optical density reached was lower. The membrane of the clsA mutant strain showed an increased PG content, at both exponential and stationary phase, but no trace of CL in either LB or LBN medium. As well as the deficiency in CL synthesis, the clsA mutant showed other differences in lipid and fatty acids content compared to the wild-type, suggesting a cross-regulation in membrane lipid pathways, crucial for the maintenance of membrane functionality and integrity. The biophysical characteristics of membranes and large unilamellar vesicles from the wild-type and clsA mutant strains were studied by Laurdan's steady-state fluorescence spectroscopy. At physiological temperature, the clsA mutant showed a decreased lateral lipid packing in the protein-free vesicles and isolated membranes compared with the wild-type strain. Interestingly, the lateral lipid packing of the membranes of both the wild-type and clsA mutant strains increased when they were grown in LBN. In a conditional IPTG-controlled pgsA mutant, unable to synthesize PG and CL in the absence of IPTG, the osmoresistance of the cultures correlated with their content of anionic phospholipids. The transcriptional activity of the clsA and pgsA genes was similar and increased twofold upon entry to stationary phase or under osmotic upshift. Overall, these results support the involvement of the anionic phospholipids in the growth of B. subtilis in media containing elevated NaCl concentrations.

show abstract

Genome‐wide analysis of the general stress response in Bacillus subtilis

Abstract: SummaryBacteria respond to diverse growth-limiting stresses by producing a large set of general stress proteins. In Bacillus subtilis and related Gram-positive pathogens, this

Cited by 251 publications

References 91 publications

MecA, an adaptor protein necessary for ClpC chaperone activity

MecA, an adaptor protein necessary for ClpC chaperone activity

A Comprehensive Proteomics and Transcriptomics Analysis ofBacillus subtilisSalt Stress Adaptation

Role of anionic phospholipids in the adaptation of Bacillus subtilis to high salinity

Contact Info

Product

Resources

About