Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism

Liu, Lecheng; Xu, Hui; Zhang, W.; Xing, Jun‐Jie; Zhu, Mingzhao; Zhang, Yuchen; Wang, Yongqin

doi:10.3390/genes14061286

Cited by 4 publications

(3 citation statements)

References 37 publications

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“…Moreover, the number of TwBAHDs in different clades was also different from that of other species. For example, Clade II only contained one member in T. mairei, which could be the orthologous gene of ECERIFERUM2 [41]. Clade III did not include the BAHD gene of T. mairei.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Pattern Analysis of BAHD Acyltransferase Family in Taxus mairei

Xu,

Wang,

Zhuang

et al. 2024

IJMS

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BAHD acyltransferases are involved in catalyzing and regulating the secondary metabolism in plants. Despite this, the members of BAHD family and their functions have not been reported in the Taxus species. In this study, a total of 123 TwBAHD acyltransferases from Taxus wallichiana var. mairei genome were identified and divided into six clades based on phylogenetic analysis, of which Clade VI contained a Taxus-specific branch of 52 members potentially involved in taxol biosynthesis. Most TwBAHDs from the same clade shared similar conserved motifs and gene structures. Besides the typical conserved motifs within the BAHD family, the YPLAGR motif was also conserved in multiple clades of T. mairei. Moreover, only one pair of tandem duplicate genes was found on chromosome 1, with a Ka/Ks ratio < 1, indicating that the function of duplicate genes did not differentiate significantly. RNA-seq analysis revealed different expression patterns of TwBAHDs in MeJA induction and tissue-specific expression experiments. Several TwBAHD genes in the Taxus-specific branch were highly expressed in different tissues of T. mairei, suggesting an important role in the taxol pathway. This study provides comprehensive information for the TwBAHD gene family and sets up a basis for its potential functions.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Pattern Analysis of BAHD Acyltransferase Family in Taxus mairei

Xu,

Wang,

Zhuang

et al. 2024

IJMS

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“…Following the molecular characterization of these genetic loci ( Tacke et al., 1995 ; Negruk et al., 1996 ; Xia et al., 1996 ), the homologous Gl2-like locus ( Alexander et al., 2020 ) and four homologous CER2-LIKE loci ( Pascal et al., 2013 ; Haslam et al., 2015 ; Haslam and Kunst, 2020 ) were identified in maize and Arabidopsis, respectively. Additionally, homologs of these proteins have been characterized from a number of different plant species, including rice ( Wang et al., 2017 ), onion ( Liu et al., 2023 ), broccoli ( Han et al., 2021 ), cabbage ( Ji et al., 2021 ), and poplar ( Gonzales-Vigil et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

The impact of the GLOSSY2 and GLOSSY2-LIKE BAHD-proteins in affecting the product profile of the maize fatty acid elongase

Alexander,

Winkelman,

Stenback

et al. 2024

Front. Plant Sci.

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The maize glossy2 and glossy2-like genes are homologs, which encode proteins that belong to the BAHD family of acyltransferases. In planta genetic studies have demonstrated that these genes may be involved in the elongation of very long chain fatty acids (VLCFAs) that are precursors of the cuticular wax fraction of the plant cuticle. VLCFAs are synthesized by a fatty acyl-CoA elongase complex (FAE) that consists of four component enzymes. Previously, we functionally identified the maize FAE component enzymes by their ability to complement haploid Saccharomyces cerevisiae strains that carry lethal deletion alleles for each FAE component enzyme. In this study we used these complemented haploid strains and wild-type diploid strains to evaluate whether the co-expression of either GLOSSY2 or GLOSSY2-LIKE with individual maize FAE component enzymes affects the VLCFA product-profile of the FAE system. Wild-type diploid strains produced VLCFAs of up to 28-carbon chain length. Co-expression of GLOSSY2 or GLOSSY2-LIKE with a combination of maize 3-ketoacyl-CoA synthases stimulated the synthesis of longer VLCFAs, up to 30-carbon chain lengths. However, such results could not be recapitulated when these co-expression experiments were conducted in the yeast haploid mutant strains that lacked individual components of the endogenous FAE system. Specifically, lethal yeast mutant strains that are genetically complemented by the expression of maize FAE-component enzymes produce VLCFAs that range between 20- and 26-carbon chain lengths. However, expressing either GLOSSY2 or GLOSSY2-LIKE in these complemented strains does not enable the synthesis of longer chain VLCFAs. These results indicate that the apparent stimulatory role of GLOSSY2 or GLOSSY2-LIKE to enable the synthesis of longer chain VLCFAs in diploid yeast cells may be associated with mixing plant enzyme components with the endogenous FAE complex.

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“…Genome-wide analyses offer profound insights into the genetic underpinnings of secondary metabolite synthesis in plants. To date, the genomes of the BAHD acyltransferase family have been delineated in a variety of species, including the Welsh Onion [22], Wurfbainia villosa [3], Lithospermum erythrorhizon [23], and Theobroma cacao [24]. Despite these advances, research on the BAHD gene family within L. japonicus remains scarce.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analysis of the BAHD gene family in Leonurus japonicus

Wang,

Zhang,

et al. 2024

Preprint

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Background Acylation represents a pivotal biochemical process that is instrumental in the modification of secondary metabolites throughout the growth and developmental stages of plants. The BAHD acyltransferase family within the plant kingdom predominantly utilizes coenzyme A thioester as the acyl donor, while employing alcohol or amine compounds as the acceptor substrates to facilitate acylation reactions. These reactions are crucial for the biosynthesis of a diverse array of products, including but not limited to lignin monomers, anthocyanins, terpenoids, and alkaloids. Despite the importance of the BAHD acyltransferases in the context of plant biochemistry, an in-depth investigation into the BAHD gene family within the species Leonurus japonicus (L. japonicus) has yet to be conducted. Results Using bioinformatics approaches, the LjBAHD gene family members in the genome of L. japonicus were identified and characterized including gene structure, conserved motifs, cis-acting elements, and potential gene functions. In total, we detected 47 LjBAHD genes, unevenly distributed across 10 chromosomes of L. japonicus, with subcellular localization predictions indicating a predominant cytoplasmic localization. Phylogenetic analysis grouped the LjBAHD genes into four distinct clades. Cis-acting element analysis revealed that the expression of BAHD genes is predominantly regulated by light and hormonal signals. Examination of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways demonstrated remarkably enrichment of LjBAHD genes in the biosynthetic pathways for Stilbenoids, diarylheptanoids and gingerol biosynthesis (Ko00945), as well as Flavonoid biosynthesis (Ko00941) and Phenylpropanoid biosynthesis (Ko00940). To elucidate the roles of BAHD genes in various tissues of L. japonicus, the expression profiles of LjBAHD family members across different organs were scrutinized. Notably, a detailed study was conducted on a specific HCT gene (i.e. LjBAHD25) within the BAHD gene family. Analysis of its expression patterns suggested a role for LjBAHD25 in the phenylpropanoid metabolism pathway in L. japonicus, contributing to the biosynthesis of secondary metabolites with unique bioactivity. Conclusion The findings of this study have established a scientific foundation for the subsequent development and functional validation of the BAHD gene family in L. japonicus.

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Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism

Cited by 4 publications

References 37 publications

Genome-Wide Identification and Expression Pattern Analysis of BAHD Acyltransferase Family in Taxus mairei

Genome-Wide Identification and Expression Pattern Analysis of BAHD Acyltransferase Family in Taxus mairei

The impact of the GLOSSY2 and GLOSSY2-LIKE BAHD-proteins in affecting the product profile of the maize fatty acid elongase

Genome-wide identification and expression analysis of the BAHD gene family in Leonurus japonicus

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