Genome-wide analysis of purple acid phosphatase structure and expression in ten vegetable species

Abstract

BackgroundAcquisition of external phosphorus (P) and optimisation of internal P are essential for plant growth and development, and insufficient availability of P in soils is a major challenge in agriculture. Members of the purple acid phosphatase (PAP) family of enzymes are candidates for increasing P use efficiency. Herein, we identified PAP homologs in the genomes of 10 vegetable species, along with Arabidopsis thaliana and Amborella trichopoda as references, to provide fundamental knowledge for this family.ResultsPhylogenetic analysis of protein sequences revealed nine distinct clades, indicating that functional differentiation of extant PAPs was established prior to the emergence of early angiosperms, and conserved among homologs in each clade. Analysis of transcript abundance in different tissues (root, stem, leaf, flower, and fruit) and following phosphates (Pi) starvation treatments from published RNA-seq transcriptome datasets facilitated comprehensive evaluation of expression patterns, and some groups of tissue-specific and Pi starvation-induced PAPs were characterised. Conserved motifs identified from upstream sequences of homologs that are highly expressed in particular tissues or following starvation treatment suggests that divergence in PAP gene expression is associated with cis-acting elements in promoters.ConclusionsThe genome-wide analysis of PAP enzyme structure and transcriptional expression patterns advance our understanding of PAP family in vegetables genomes. Therefore, PAP homologs with known enzyme structures and expression profiles could serve as targets for plant breeding and/or genetic engineering programs to improve P acquisition and use.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5022-1) contains supplementary material, which is available to authorized users.

Genotype-Specific Differences in Phosphorus Efficiency of Potato (Solanum tuberosum L.)

“…A large group of purple acid phosphatases, which catalyze phosphate ester hydrolysis, have been described in Arabidopsis (Li et al, 2002), of which three are the major secreted and root-associated phosphatases under P deficiency (Wang et al, 2014). Twenty-two purple acid phosphatases were found in potato (Xie and Shang, 2018). The expressions of three potato purple acid phosphatases were characterized by Zimmermann et al (2004), of which two responded to P deficiency with increased expressions in roots.…”

Vigna radiata (mung bean) acid phosphatase is difficult to purify and may have a role as a vegetative Storage protein

“…reports of acid phosphatase isolation from plants describe either a low molecular weight enzyme of around 30 kDa (Zaman et al, 2020;Saeed et al, 2014;Nadir et al, 2012;Asaduzzaman et al, 2011;Zaman et al, 2020) or a higher molecular weight enzyme around 60 kDa (Anand and Srivastava, 2014;Xie and Shang, 2018). This study produced a low molecular weight AP enzyme.…”

Building a Flower: The Influence of Cell Wall Composition on Flower Development and Reproduction

“…However, the function of proteins involved in the formation, development, or arrangement of cell walls is poorly understood in floral tissues. Although, based on results of promoter activity, gene expression, or immunolocalization of cell wall components, it gives us an idea that some of them could be involved in floral organ development [ 6 , 108 , 110 , 111 , 112 , 113 , 114 , 115 ].…”