Genome-Wide Analysis of Long Noncoding RNA Profile in Human Gastric Epithelial Cell Response to Helicobacter pylori

Abstract

SUMMARY: Long noncoding RNAs (lncRNAs) are an important class of pervasive genes, and their misregulation has been shown in various types of diseases. However, the relationship between lncRNAs and the immune response to pathogen infection has been rarely reported. Helicobacter pylori is a major human pathogenic bacterium that causes gastritis, peptic ulcer disease, mucosa-associated lymphoid tissue lymphoma, and gastric cancer. The regulatory mechanism of the H. pylori-induced immune response is not yet clear. In the present study, we identified nonoverlapping signatures of a small number of lncRNAs that were aberrantly expressed in H. pylori-infected gastric epithelial cells using microarray analysis followed by bioassays. From microarray data, we found that 23 lncRNAs were upregulated and 21 were downregulated. Five lncRNAs, XLOC_004562, XLOC_005912, XLOC_000620, XLOC_004122, and XLOC_014388, were further evaluated using quantitative reverse transcription-PCR, and the results matched well with microarray data. In addition, XLOC_004122 and XLOC_ 014388 were decreased in gastric mucosal tissues of H. pylori-positive patients. Differentially expressed lncRNAs may play a partial or key role in the immune response to H. pylori, and this may provide potential targets for the future treatment of H. pylori-related diseases.

<p>Long noncoding RNA THAP9-AS1 is induced by <em>Helicobacter pylori</em> and promotes cell growth and migration of gastric cancer</p>

“…Though the expression pattern of lncRNAs upon H. pylori infection of a gastric epithelial cell line, GES-1, has been studied, the expression pattern of lncRNAs influenced by H. pylori in GES-1 cells was explored using a microarray, revealing the relationship between lncRNAs and H. pylori-associated diseases. 39,40 We explored the expression pattern of lncRNAs in H. pylori-infected AGS cells using RNA-seq which revealed a full-scale lncRNAs map for H. pylori-infected AGS cells and identified seven to eight-times more lncRNAs than microarray. Meanwhile, our study also revealed the lncRNAs expression in H. pylori infected GC cells, which was not reported yet.…”

Characterization of long non-coding RNA-associated ceRNA network to reveal potential prognostic lncRNA biomarkers in human ovarian cancer

“…The differential expressions of lncRNAs have been widely observed in various cancers [ 34 - 37 ], and their expressional perturbation has been implicated in the development and progression of cancers [ 38 , 39 ]. Several lncRNA signatures have been developed to improve prognosis prediction of cancers, including colorectal cancer [ 40 ], glioblastoma multiforme [ 41 ], breast cancer [ 42 ], lung cancer [ 43 ] and multiple myeloma [ 44 ].…”

A Long Journey Ahead: Long Non-coding RNAs in Bacterial Infections

“…monocytogenes, Helicobacter pylori , and Campylobacter ( C .) concisus as well as TLR ligands has been recently demonstrated in several studies but the functions of these lncRNAs remain to be elucidated (Ilott et al, 2014; Yi et al, 2014; Yang et al, 2015; Zhu et al, 2015; Yang R. et al, 2016; Westermann et al, 2016). …”