Genome-Wide Analysis of Late Embryogenesis Abundant Protein Gene Family in Vigna Species and Expression of VrLEA Encoding Genes in Vigna glabrescens Reveal Its Role in Heat Tolerance

Abstract

Late embryogenesis abundant (LEA) proteins are identified in many crops for their response and role in adaptation to various abiotic stresses, such as drought, salinity, and temperature. The LEA genes have been studied systematically in several crops but not in Vigna crops. In this study, we reported the first comprehensive analysis of the LEA gene family in three legume species, namely, mung bean (Vigna radiata), adzuki bean (Vigna angularis), and cowpea (Vigna unguiculata), and the cross-species expression of VrLEA genes in a wild tetraploid species, Vigna glabrescens. A total of 201 LEA genes from three Vigna crops were identified harboring the LEA conserved motif. Among these 55, 64, and 82 LEA genes were identified in mung bean, adzuki bean, and cowpea genomes, respectively. These LEA genes were grouped into eight different classes. Our analysis revealed that the cowpea genome comprised all eight classes of LEA genes, whereas the LEA-6 class was absent in the mung bean genome. Similarly, LEA-5 and LEA-6 were absent in the adzuki bean genome. The analysis of LEA genes provides an insight into their structural and functional diversity in the Vigna genome. The genes, such as VrLEA-2, VrLEA-40, VrLEA-47, and VrLEA-55, were significantly upregulated in the heat-tolerant genotype under stress conditions indicating the basis of heat tolerance. The successful amplification and expression of VrLEA genes in V. glabrescens indicated the utility of the developed markers in mung bean improvement. The results of this study increase our understanding of LEA genes and provide robust candidate genes for future functional investigations and a basis for improving heat stress tolerance in Vigna crops.

Genome-wide identification, comprehensive characterization of transcription factors, cis-regulatory elements, protein homology, and protein interaction network of DREB gene family in Solanum lycopersicum

“…Histone acetylation is essential in the cell cycle and in response to abiotic stress (light, heat, UVB, sugar, salt, and wound) and hormonal change (salicylic, ethylene, abscisic acid and auxin) ( Jiang et al., 2020 ). Histone acetyltransferase genes previously discovered in rice ( Liu et al., 2012 ), Triticum aestivum ( Gao et al., 2021 ), and Arabidopsis thaliana ( Pandey et al., 2002 ) (Solyc02g077970.2.1) belong to the LEA protein family which are connected with salinity and water stress signaling and have been reported in many plants like Vigna glabrescens ( Singh et al., 2022 ), cotton ( Magwanga et al., 2018 ), Salvia miltiorrhiza ( Chen et al., 2021 ), and Triticum aestivum ( Liu et al., 2019 ). The presence of AP2 domain was detected in one member (Solyc03g044300.2.1) that contributed to transcriptional regulation and responded to stresses as found in wheat ( Magar et al., 2022 ) and castor bean ( Xu et al., 2013 ).…”

Multi-environment Genome Wide Association Studies of Yield Traits in Common Bean (Phaseolus vulgaris L.) × Tepary Bean (P. acutifolius A. Gray) Interspecific Advanced Lines at the Humid and Dry Colombian Caribbean Subregions

“…We found a relevant protective protein named Late embryogenesis abundant (LEA) (Chen et al, 2021) (Phvul.011G001700) only in the RS Motilonia using the SNP marker S11_140976, in other legume of importance such as Vigna sp. has been reported up regulation of some genes grouped like LEA in heat stress tolerant genotypes (Singh et al, 2022). Therefore, we found the regulatory protein MYB in RS Turipaná (Phvul.008G205000) and Caribia (Phvul.002G088900), particularly a tranbscription factor in the biosynthesis of phenylpropanoids , an enrichment pathway that we found too (Deng and Lu, 2017; Yang et al, 2021).…”

Analisa Perbandingan Perkuatan Tanah Soil Nailing dan Shotcrete pada Proyek Gelanggang Inovasi dan Kreativitas Universitas Gadjah Mada

“…Shotcrete semakin penting karena kemampuan beradaptasi, ketangguhan, kekuatan tinggi, ikatan yang baik, dan kemudahan aplikasi pada permukaan penerima di area yang sulit diakses (Yuliarti & Septita, 1996). Shotcrete digunakan untuk terowongan bawah tanah, perbaikan struktur bangunan, dan perlindungan lereng atau permukaan (tunnel) berkaitan dengan kekuatannya, keberhasilan penerapannya, dan banyak manfaat yang ditawarkannya (Singh et al, 2022). Tujuan dari penelitian ini adalah untuk mengetahui perbandingan biaya dalam pekerjaan soil nailing dan shotcrete, mengetahui latar belakang pemilihat tempat serta mengetahui metode pelaksanaan soil nailing dan shotcrete (Nasional, 2017).…”