Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene

Guimarães, Larissa Arrais; Mota, Ana Paula Zotta; Araújo, Ana Cláudia Guerra; Figueiredo, Lúcio Flávio de Alencar; Pereira, Bruna Medeiros; Saraiva, Mario Alfredo de Passos; Silva, Raquel Bispo; Danchin, Etienne G. J.; Guimarães, P. M.; Brasileiro, Ana Cristina Miranda

doi:10.1007/s11103-017-0594-8

Cited by 42 publications

(43 citation statements)

References 73 publications

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“…2h). To further support current results, the overexpression of AdEXLB8 in soybean ( Glycine max ) composite plants infected with M. javanica also led to the reduction of the gall number in the susceptible genotype [27]. However, the biological mechanisms by which the overexpression of this cell wall-loosening and non-enzymatic protein contributes to RKN-resistance in peanut and soybean must be pursued.…”

Section: Resultsmentioning

confidence: 87%

Ex vitro hairy root induction in detached peanut leaves for plant–nematode interaction studies

et al. 2017

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BackgroundPeanut (Arachis hypogaea) production is largely affected by a variety of abiotic and biotic stresses, including the root-knot nematode (RKN) Meloidogyne arenaria that causes yield losses worldwide. Transcriptome studies of wild Arachis species, which harbor resistance to a number of pests and diseases, disclosed several candidate genes for M. arenaria resistance. Peanut is recalcitrant to genetic transformation, so the use of Agrobacterium rhizogenes-derived hairy roots emerged as an alternative for in-root functional characterization of these candidate genes.ResultsThe present report describes an ex vitro methodology for hairy root induction in detached leaves based on the well-known ability of peanut to produce roots spontaneously from its petiole, which can be maintained for extended periods under high-humidity conditions. Thirty days after infection with the A. rhizogenes ‘K599’ strain, 90% of the detached leaves developed transgenic hairy roots with 5 cm of length in average, which were then inoculated with M. arenaria. For improved results, plant transformation, and nematode inoculation parameters were adjusted, such as bacterial cell density and growth stage; moist chamber conditions and nematode inoculum concentration. Using this methodology, a candidate gene for nematode resistance, AdEXLB8, was successfully overexpressed in hairy roots of the nematode-susceptible peanut cultivar ‘Runner’, resulting in 98% reduction in the number of galls and egg masses compared to the control, 60 days after M. arenaria infection.ConclusionsThis methodology proved to be more practical and cost-effective for functional validation of peanut candidate genes than in vitro and composite plant approaches, as it requires less space, reduces analysis costs and displays high transformation efficiency. The reduction in the number of RKN galls and egg masses in peanut hairy roots overexpressing AdEXLB8 corroborated the use of this strategy for functional characterization of root expressing candidate genes. This approach could be applicable not only for peanut–nematode interaction studies but also to other peanut root diseases, such as those caused by fungi and bacteria, being also potentially extended to other crop species displaying similar petiole-rooting competence.

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Section: Resultsmentioning

confidence: 87%

Ex vitro hairy root induction in detached peanut leaves for plant–nematode interaction studies

et al. 2017

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“…Unlike the paralogs of clades EXPA‐I, EXPA‐III and EXPA‐IV, SppEXPB1 and SppEXPB2 are not on separate pseudomolecules (v2 genome) or separate scaffolds (v3 genome), but rather are found in tandem. They are the only example of tandemly located expansins in Spirodela , which is a deviation from other angiosperm expansin families that often contain numerous instances of tandemly duplicated expansin genes (Sampedro and Cosgrove, ; Dal Santo et al , ; Zhu et al , ; Zhang et al , ; Krishnamurthy et al , ; Lu et al , ; Guimaraes et al , ).…”

Section: Resultsmentioning

confidence: 99%

“…No concrete function has been described for the expansin‐like B family (Sampedro and Cosgrove, ; Cosgrove, ), making it difficult to speculate as to what role(s) EXLBs may be fulfilling in terrestrial plants. In a unique scenario, Guimaraes et al identified an EXLB gene ( EXLB8 ) in Arachis that perturbs nematode infection when transiently expressed in the roots of Glycine max (Guimaraes et al , ); however, those results describe a single EXLB gene that may not be representative of the family as a whole (e.g. gain of function).…”

Section: Discussionmentioning

confidence: 99%

Expansin gene loss is a common occurrence during adaptation to an aquatic environment

et al. 2019

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Expansins comprise a superfamily of plant cell wall loosening proteins that can be divided into four individual families (EXPA, EXPB, EXLA and EXLB). Aside from inferred roles in a variety of plant growth and developmental traits, little is known regarding the function of specific expansin clades, for which there are at least 16 in flowering plants (angiosperms); however, there is evidence to suggest that some expansins have cell-specific functions, in root hair and pollen tube development, for example. Recently, two duckweed genomes have been sequenced (Spirodela polyrhiza strains 7498 and 9509), revealing significantly reduced superfamily sizes. We hypothesized that there would be a correlation between expansin loss and morphological reductions seen among highly adapted aquatic species. In order to provide an answer to this question, we characterized the expansin superfamilies of the greater duckweed Spirodela, the marine eelgrass Zostera marina and the bladderwort Utricularia gibba. We discovered rampant expansin gene and clade loss among the three, including a complete absence of the EXLB family and EXPA-VII. The most convincing correlation between morphological reduction and expansin loss was seen for Utricularia and Spirodela, which both lack root hairs and the root hair expansin clade EXPA-X. Contrary to the pattern observed in other species, four Utricularia expansins failed to branch within any clade, suggesting that they may be the result of neofunctionalization. Last, an expansin clade previously discovered only in eudicots was identified in Spirodela, allowing us to conclude that the last common ancestor of monocots and eudicots contained a minimum of 17 expansins.

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“…3). This uneven distribution on the chromosomes is ubiquitous whether in monocotyledonous or dicotyledonous plants [29,34,35], and there are certain tendencies of distribution on different chromosomes. For example, several EXLB genes had a clustered distribution in Ghir_A08 and Ghir_D08, and expansin genes from the same subfamily were distributed on the majority of chromosomes, such as EXPAs in Ghir_A05 (Fig.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of expansin gene family reveals expansin genes are involved in fibre cells growth in cotton

Zuo

Wang

et al. 2019

Preprint

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Background : Expansins ( EXPs ), a group of proteins that loosen plant cell walls and cellulosic materials, are involved in regulating cell growth and diverse developmental processes in plants. However, the biological functions of this gene family are still unknown in cotton. Results: In this paper, we identified a total of 93 expansin genes in Gossypium hirsutum . These genes were classified into four subfamilies, including 67 GhEXPAs , eight GhEXPBs , six GhEXLAs , and 12 GhEXLBs , and divided into 15 subgroups. All 93 expansin genes are distributed over 24 chromosomes excluding Ghir_A02 and Ghir_D06. All GhEXP genes contain multiple exons and each GhEXP protein has multiple conserved motifs. Transcript profiling and qPCR analysis revealed that the expansin genes have distinct expression patterns in different stages of cotton fibre development. Among them, three genes ( GhEXPA4o , GhEXPA1A , and GhEXPA8h ) were highly expressed in the initiation stage, nine genes ( GhEXPA4a , GhEXPA13a , GhEXPA4f , GhEXPA4q , GhEXPA8f , GhEXPA2 , GhEXPA8g , GhEXPA8a , and GhEXPA4n ) had high expression during the fast elongation stage, while GhEXLA1c and GhEXLA1f were preferentially expressed in the transition stage of fibre development. Conclusions: Our results provide a solid basis for further elucidation of biological functions of expansin genes in cotton fibre development and valuable genetic resources used for crop improvement in the future.

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Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene

Cited by 42 publications

References 73 publications

Ex vitro hairy root induction in detached peanut leaves for plant–nematode interaction studies

Ex vitro hairy root induction in detached peanut leaves for plant–nematode interaction studies

Expansin gene loss is a common occurrence during adaptation to an aquatic environment

Genome-wide identification of expansin gene family reveals expansin genes are involved in fibre cells growth in cotton

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