Genome-Wide Analysis of ATP Binding Cassette (ABC) Transporters in Peach (Prunus persica) and Identification of a Gene PpABCC1 Involved in Anthocyanin Accumulation

Abstract

The ATP-binding cassette (ABC) transporter family is a large and diverse protein superfamily that plays various roles in plant growth and development. Although the ABC transporters are known to aid in the transport of a wide range of substrates across biological membranes, their role in anthocyanin transport remains elusive. In this study, we identified a total of 132 putative ABC genes in the peach genome, and they were phylogenetically classified into eight subfamilies. Variations in spatial and temporal gene expression levels resulted in differential expression patterns of PpABC family members in various tissues of peach. PpABCC1 was identified as the most likely candidate gene essential for anthocyanin accumulation in peach. Transient overexpression of PpABCC1 caused a significant increase in anthocyanin accumulation in tobacco leaves and peach fruit, whereas virus-induced gene silencing of PpABCC1 in the blood-fleshed peach resulted in a significant decrease in anthocyanin accumulation. The PpABCC1 promoter contained an MYB binding cis-element, and it could be activated by anthocyanin-activator PpMYB10.1 based on yeast one-hybrid and dual luciferase assays. Thus, it seems that PpABCC1 plays a crucial role in anthocyanin accumulation in peach. Our results provide a new insight into the vacuolar transport of anthocyanins in peach.

Comprehensive genome-wide identification and characterization of ABC Transporter superfamily in Cajanus cajan and its expression profiling under different developmental stages in various anatomical tissues

“…Similarly, the molecular weight of the CcABC proteins ranged from 6.97 to 345.38. The isoelectric point of the CcABC proteins lies in the range of 4.44 to 10.74 in the same manner as it has been reported in the ABC proteins of plants like Zea mays (Guo et al, 2022), 5.08 to 9.74 strawberry (Shi et al, 2020), 5.4 to 8.96 in cotton (Sun N et al 2021), 4.41 to 10.5 in soyabean (Mishra et al 2019) 5.05 to 10.48 in peach (Sylvia et al 2023)and tomato (Ofori PA et al 2018). In C. cajan, out of 159 CcABC proteins, 105 proteins were basic and 50 were acidic in nature, whereas 2 were neutral proteins.…”

“…All the motifs of ABC gene family were highly conserved as reported in peach (Sylvia C et al, 2023), brassica rapa (Yan C et al2017), zea mays (Guo Z et al 2022), soyabean (Huang J et al 2021) and capsicum (Ortiz C et al 2019). It were also mentioned in the pineapple (Chen P et al 2017), hexaploid wheat (Bhati KK et al 2015), barley (Zhang Z et al 2020), strawberry (M. Shi et al, 2020), and flax (Khan et al2020) that all the conserved motifs were identified.…”

“…In CcABC genes, the stress-responsive elements MYB, MYC, STRE, and WRE3 were the most common. The element LTR has been reported to be involved in low-temperature responsiveness, ARE has been found responsible for anaerobic induction (Sylvia et al 2023). In a similar manner, the elements WUN, and MBS have their function common in both rosacea and Cajanuscajan(Ortiz et al 2019) (Ijaz et al 2020).…”

“…The presence of light-responsive elements suggests that they actively take part in photosynthesis. The GCN4 and O2 site are involved in endosperm expression, and zein metabolism (Sylvia et al 2023).…”

“…The CARE analysis of the CcABC promoter region revealed different elements, which were divided into broad categories of core, stress, hormone, cellular, and light-responsive CARE elements (Vaish et al 2022) (Yan C et al 2017), (Sun N et al 2021). In C. cajan, the majority of the CARE were found to be involved in the biotic and abiotic stresses in the same way it was found in peach (Kaur et al 2017) (Sylvia et al 2023), (Mishra et al 2019). A total of 23 common CARE elements were in all three of the capsicum (Ortiz C et al 2019) and 13 in glycine max (Mishra et al 2019), 17 in (Sun N et al 2021), whereas in CcABC genes a total of 26 CARE elements were found.…”