Genome-wide analysis of a cellular exercise model based on electrical pulse stimulation

Lee, Bora; Kim, Seon‐Kyu; Shin, Yeo Jin; Son, Young Hoon; Yang, Jae Won; Lee, Seungmin; Yang, Yong Ryul; Lee, Kwang-Pyo; Kwon, Ki‐Sun

doi:10.1038/s41598-022-25758-2

Cited by 2 publications

(2 citation statements)

References 61 publications

(55 reference statements)

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“…We have previously argued that the EPS model mimic exercise quite well, at least when it comes to metabolic effects ( Nikolic et al, 2017 ). Recently, a comparative analysis of exercise signature genes in skeletal muscles after in vivo exercise and in vitro EPS also confirmed that the EPS model mimicked in vivo exercise at the transcriptome level ( Lee et al, 2022 ). Altogether, the EPS model of human myotubes appears to be useful and relevant.…”

Section: Discussionmentioning

confidence: 80%

Distinct microRNA and protein profiles of extracellular vesicles secreted from myotubes from morbidly obese donors with type 2 diabetes in response to electrical pulse stimulation

et al. 2023

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Lifestyle disorders like obesity, type 2 diabetes (T2D), and cardiovascular diseases can be prevented and treated by regular physical activity. During exercise, skeletal muscles release signaling factors that communicate with other organs and mediate beneficial effects of exercise. These factors include myokines, metabolites, and extracellular vesicles (EVs). In the present study, we have examined how electrical pulse stimulation (EPS) of myotubes, a model of exercise, affects the cargo of released EVs. Chronic low frequency EPS was applied for 24 h to human myotubes isolated and differentiated from biopsy samples from six morbidly obese females with T2D, and EVs, both exosomes and microvesicles (MV), were isolated from cell media 24 h thereafter. Size and concentration of EV subtypes were characterized by nanoparticle tracking analysis, surface markers were examined by flow cytometry and Western blotting, and morphology was confirmed by transmission electron microscopy. Protein content was assessed by high-resolution proteomic analysis (LC-MS/MS), non-coding RNA was quantified by Affymetrix microarray, and selected microRNAs (miRs) validated by real time RT-qPCR. The size and concentration of exosomes and MV were unaffected by EPS. Of the 400 miRs identified in the EVs, EPS significantly changed the level of 15 exosome miRs, of which miR-1233-5p showed the highest fold change. The miR pattern of MV was unaffected by EPS. Totally, about 1000 proteins were identified in exosomes and 2000 in MV. EPS changed the content of 73 proteins in exosomes, 97 in MVs, and of these four were changed in both exosomes and MV (GANAB, HSPA9, CNDP2, and ATP5B). By matching the EPS-changed miRs and proteins in exosomes, 31 targets were identified, and among these several promising signaling factors. Of particular interest were CNDP2, an enzyme that generates the appetite regulatory metabolite Lac-Phe, and miR-4433b-3p, which targets CNDP2. Several of the regulated miRs, such as miR-92b-5p, miR-320b, and miR-1233-5p might also mediate interesting signaling functions. In conclusion, we have used a combined transcriptome-proteome approach to describe how EPS affected the cargo of EVs derived from myotubes from morbidly obese patients with T2D, and revealed several new factors, both miRs and proteins, that might act as exercise factors.

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Section: Discussionmentioning

confidence: 80%

Distinct microRNA and protein profiles of extracellular vesicles secreted from myotubes from morbidly obese donors with type 2 diabetes in response to electrical pulse stimulation

et al. 2023

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“…Overall, this suggests that NR4A3 has a critical role in SkM metabolism and adaptation to changes in physical activity (23). In addition to NR4A3, amphiregulin (Areg) has been shown to be upregulated after EL-EPS in C2C12 myotubes and in SkM of exercised mice (24) suggesting its´ role as a novel exerkine.…”

Section: D In Vitro Exercise and Transcriptomicsmentioning

confidence: 95%

Mimicking exercise in vitro: effects of myotube contractions and mechanical stretch on omics

Lautaoja

Turner

Sharples

et al. 2023

American Journal of Physiology-Cell Physiology

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The number of studies using skeletal muscle (SkM) cell culture models to study exercise in vitro are rapidly expanding. Progressively, more comprehensive analysis methods, such as different omics approaches including transcriptomics, proteomics and metabolomics have been used to examine the intra- and extracellular molecular responses to exercise mimicking stimuli in cultured myotubes. Among other techniques, exercise-like electrical pulse stimulation (EL-EPS) and mechanical stretch of SkM cells are the two most commonly used methods to mimic exercise in vitro. In this mini-review we focus on these two approaches and their effects on the omics of myotubes and/or cell culture media. Furthermore, besides traditional two-dimensional (2D) methods, the use of three-dimensional (3D) SkM approaches are increasing in the field of in vitro exercise mimicry. Our aim with this mini-review is to provide the reader with an up-to-date overview of the 2D and 3D models and the use of omics approaches to study the molecular response to exercise in vitro.

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Genome-wide analysis of a cellular exercise model based on electrical pulse stimulation

Cited by 2 publications

References 61 publications

Distinct microRNA and protein profiles of extracellular vesicles secreted from myotubes from morbidly obese donors with type 2 diabetes in response to electrical pulse stimulation

Distinct microRNA and protein profiles of extracellular vesicles secreted from myotubes from morbidly obese donors with type 2 diabetes in response to electrical pulse stimulation

Mimicking exercise in vitro: effects of myotube contractions and mechanical stretch on omics

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