Genome-Wide Analysis Identifies Candidate Genes Encoding Beak Color of Duck

Guo, Qixin; Jiang, Yong; Wang, Zhixiu; Bi, Yue; Chen, Guohong; Bai, Hao; Chang, Guobin

doi:10.3390/genes13071271

Cited by 9 publications

(4 citation statements)

References 67 publications

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“…Consequently, more than 50 genes linked to plumage coloration have been identi ed in avian species [9]. Notably, several of these genes, including melanocortin receptor 1 (MC1R) gene [10][11][12], tyrosinase (TYR) gene [12,13], tyrosinase-related protein 1(TYRP1) gene [14], Endothelin Receptor B2 (EDNRB2) gene [15], Dopachrome tautomerase (DCT) gene [16], Microphthalmia-associated transcription factor (MITF) [17] gene, among others, have been extensively investigated for their role in modulating melanogenesis, melanoblast migration, and melanocyte differentiation, thus in uencing melanin-based plumage coloration. Moreover, comprehensive studies have probed the interactions between polymorphic sites within these candidate genes and feather color across different duck populations and breeds [18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Sexual Dimorphism in Dorsal Down Coloration in Goslings

Liu,

Li,

Guo

et al. 2023

Preprint

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Background Distinctive sexual dimorphism in dorsal down coloration is apparent in day-old Hungarian white goose goslings, characterized by darker dorsal plumage in females and lighter plumage in males. The ability to autosex day-old goslings based on their dorsal down coloration is crucial for efficient management and nutrition strategies in the poultry industry. The objective of this research was to gain a deeper understanding of the potential biological and genetic mechanisms governing sexually dimorphic down coloration in geese, utilizing tissue slice analysis, biochemical assays, transcriptomic profiling, and q-PCR analysis. Results The tissue slice and biochemical results revealed that female geese exhibited a higher density of melanin-containing feather follicles and a greater concentration of melanin within these follicles compared to male geese during embryonic development at 17 days and in day-old goslings. Both female and male individuals, in comparison to 17-day goose embryos, showed a significant decrease in melanin concentrations in the dorsal skin of day-old goslings. Through transcriptome analysis, we identified a set of differentially expressed genes (DEGs) (MC1R, TYR, TYRP1, DCT and MITF) associated with Melanogenesis pathways that exhibit substantial down-regulation or complete silencing specifically within the dorsal skin of day-old goslings when compared to 17-day embryos. This resulting in impaired melanin synthesis in the feather follicle. Additionally, sexual dimorphism in feather coloration is associated with two key DEGs (MC1R and MITF). Female geese exhibited higher expression levels of MC1R and MITF, positively correlated with increased melanin synthesis and deposition, resulting in a more pronounced plumage phenotype characterized by darker coloration. Conclusions Melanin synthesis in goose feather follicles was determined by the expression of multiple melanogenesis genes. Sexual dimorphism in dorsal down coloration of day-old Hungarian white goose goslings was contingent upon two essential MC1R and MITF genes. Our research lays the essential foundation for advancing the breeding in autosexing geese, promising substantial benefits to breeders and producers.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Sexual Dimorphism in Dorsal Down Coloration in Goslings

Liu,

Li,

Guo

et al. 2023

Preprint

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“…In poultry, beak color is thought to be regulated by two pairs of autosomal alleles (C/c and Mb/mb; c has a recessive epistatic effect on mb) ( Jialang, 2016 ). Assisted by recent rapid developments in genetics and genomics, several single-nucleotide polymorphisms in MITF , EDNRB2 , ASIP , MC1R , and POU2F3 have been significantly associated with the regulation of melanin synthesis and beak color of ducks ( Guo et al, 2022 ; Liu et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of SNPs in MITF associated with beak color of duck

Pan,

Hua,

Guo

et al. 2023

Front. Genet.

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Introduction: Beak color—a pigment-related trait—is an important feature of duck breeds. Recently, little research has addressed genetic mechanism of the beak colors in poultry, whereas the process and the regulation factors of melanin deposition have been well described.Methods: To investigate the genetic mechanism of beak colors, we conducted an integrated analysis of genomic selection signatures to identify a candidate site associated with beak color. For this, we used black-billed (Yiyang I meat duck synthetic line H1, H2, H3&HF) and yellow-billed ducks (Cherry Valley ducks and white feather Putian black duck). Quantitative real-time PCR and genotyping approaches were used to verify the function of the candidate site.Results: We identified 3,895 windows containing 509 genes. After GO and KEGG enrichment analysis, nine genes were selected. Ultimately, MITF was selected by comparing the genomic differentiation (FST). After loci information selection, 41 extreme significantly different loci were selected, which are all located in intron regions of MITF and are in almost complete linkage disequilibrium. Subsequently, the site ASM874695v1:10:g.17814522T > A in MITF was selected as the marker site. Furthermore, we found that MITF expression is significantly higher in black-beaked ducks than in yellow-beaked ducks of the F2 generation (p < 0.01). After genotyping, most yellow-billed individuals are found with homozygous variant; at the same time, there are no birds with homozygous variant in black-billed populations, while the birds with homozygous and heterozygous variant share the same proportion.Conclusion:MITF plays a very critical role in the melanogenesis and melanin deposition of duck beaks, which can effectively affect the beak color. The MITF site, ASM874695v1:10:g.17814522T > A could be selected as a marker site for the duck beak color phenotype.

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“…Consequently, more than 50 genes linked to plumage coloration have been identified in avian species [8]. Several of these genes, including the melanocortin receptor 1 (MC1R) gene [9][10][11], tyrosinase (TYR ) gene [11,12], tyrosinase-related protein 1 (TYRP1) gene [13], endothelin receptor B2 (EDNRB2) gene [14], dopachrome tautomerase (DCT) gene [15], and microphthalmia-associated transcription factor (MITF) [16] gene, among others, have been extensively investigated for their role in modulating melanogenesis, melanoblast migration, and melanocyte differentiation, thereby influencing melaninbased plumage coloration. Moreover, comprehensive studies have investigated how different variants within these candidate genes interact with feather color in various duck populations and breeds [17][18][19][20][21][22].…”

mentioning

confidence: 99%

Transcriptome analysis of sexual dimorphism in dorsal down coloration in goslings

Liu,

Li,

Guo

et al. 2024

BMC Genomics

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Background In day-old Hungarian white goose goslings, there is a noticeable difference in dorsal down coloration between males and females, with females having darker dorsal plumage and males having lighter plumage. The ability to autosex day-old goslings based on their dorsal down coloration is important for managing them efficiently and planning their nutrition in the poultry industry. The aim of this study was to determine the biological and genetic factors underlying this difference in dorsal down colorationthrough histological analysis, biochemical assays, transcriptomic profiling, and q‒PCR analysis. Results Tissue analysis and biochemical assays revealed that compared with males, 17-day-old embryos and day-old goslings of female geese exhibited a greater density of melanin-containing feather follicles and a greater melanin concentration in these follicles during development. Both female and male goslings had lower melanin concentrations in their dorsal skin compared to 17-day-old embryos. Transcriptome analysis identified a set of differentially expressed genes (DEGs) (MC1R, TYR, TYRP1, DCT and MITF) associated with melanogenesis pathways that were downregulated or silenced specifically in the dorsal skin of day-old goslings compared to 17-day-old embryos, affecting melanin synthesis in feather follicles. Additionally, two key genes (MC1R and MITF) associated with feather coloration showed differences between males and females, with females having higher expression levels correlated with increased melanin synthesis and darker plumage. Conclusion The expression of multiple melanogenesis genes determines melanin synthesis in goose feather follicles. The dorsal down coloration of day-old Hungarian white goose goslings shows sexual dimorphism, likely due to differences in the expression of the MC1R and MITF genes between males and females. These results could help us better understand why male and female goslings exhibit different plumage patterns.

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Genome-Wide Analysis Identifies Candidate Genes Encoding Beak Color of Duck

Cited by 9 publications

References 67 publications

Transcriptome Analysis of Sexual Dimorphism in Dorsal Down Coloration in Goslings

Transcriptome Analysis of Sexual Dimorphism in Dorsal Down Coloration in Goslings

Identification of SNPs in MITF associated with beak color of duck

Transcriptome analysis of sexual dimorphism in dorsal down coloration in goslings

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