Genome-wide analysis and transcript profiling identify several abiotic and biotic stress-responsive Glutathione S-transferase genes in soybean

Hasan, Soyib; Islam, Shiful; Hasan, Nahid; Sajib, Saikat Das; Ahmed, Shamim; Islam, Tahmina; Ghosh, Ajit

doi:10.1016/j.plgene.2020.100239

Cited by 13 publications

(8 citation statements)

References 63 publications

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“…There was no StALDH gene in Chromosome 10 and 11. The expansion of StALDH gene families could be justified through gene duplication analysis 24 (Fig. 2 and Table S2 ).…”

Section: Resultsmentioning

confidence: 99%

Genome-wide identification, evolution, and transcript profiling of Aldehyde dehydrogenase superfamily in potato during development stages and stress conditions

Islam

Hasan

et al. 2021

Sci Rep

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The Aldehyde dehydrogenase (ALDH) superfamily comprises a group of enzymes involved in the scavenging of toxic aldehyde molecules by converting them into their corresponding non-toxic carboxylic acids. A genome-wide study in potato identified a total of 22 ALDH genes grouped into ten families that are presented unevenly throughout all the 12 chromosomes. Based on the evolutionary analysis of ALDH proteins from different plant species, ALDH2 and ALDH3 were found to be the most abundant families in the plant, while ALDH18 was found to be the most distantly related one. Gene expression analysis revealed that the expression of StALDH genes is highly tissue-specific and divergent in various abiotic, biotic, and hormonal treatments. Structural modelling and functional analysis of selected StALDH members revealed conservancy in their secondary structures and cofactor binding sites. Taken together, our findings provide comprehensive information on the ALDH gene family in potato that will help in developing a framework for further functional studies.

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“…There was no StALDH gene in Chromosome 10 and 11. The expansion of StALDH gene families could be justified through gene duplication analysis 24 (Fig. 2 and Table S2 ).…”

Section: Resultsmentioning

confidence: 99%

Genome-wide identification, evolution, and transcript profiling of Aldehyde dehydrogenase superfamily in potato during development stages and stress conditions

Islam

Hasan

et al. 2021

Sci Rep

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“…The number of identified GST genes are higher in Medicago as compared to 79 GST genes in rice [ 62 ], 55 in Arabidopsis [ 63 ], 84 in barley [ 64 ], 42 in maize [ 65 ], 49 in C . rubella [ 66 ], 82 in radish [ 67 ], 90 in potato [ 68 ], 90 in tomato [ 69 ], 85 in pepper [ 70 ]; but fewer than wheat with 330 GSTs [ 56 ] and soybean with 126 GSTs [ 71 ]. There is no direct correlation with the number of GST family members and their respective plant genome size ( Table 5 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and expression profiling of glutathione S-transferase family under multiple abiotic and biotic stresses in Medicago truncatula L.

et al. 2021

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Glutathione transferases (GSTs) constitute an ancient, ubiquitous, multi-functional antioxidant enzyme superfamily that has great importance on cellular detoxification against abiotic and biotic stresses as well as plant development and growth. The present study aimed to a comprehensive genome-wide identification and functional characterization of GST family in one of the economically important legume plants—Medicago truncatula. Here, we have identified a total of ninety-two putative MtGST genes that code for 120 proteins. All these members were classified into twelve classes based on their phylogenetic relationship and the presence of structural conserved domain/motif. Among them, 7 MtGST gene pairs were identified to have segmental duplication. Expression profiling of MtGST transcripts revealed their high level of organ/tissue-specific expression in most of the developmental stages and anatomical tissues. The transcripts of MtGSTU5, MtGSTU8, MtGSTU17, MtGSTU46, and MtGSTU47 showed significant up-regulation in response to various abiotic and biotic stresses. Moreover, transcripts of MtGSTU8, MtGSTU14, MtGSTU28, MtGSTU30, MtGSTU34, MtGSTU46 and MtGSTF8 were found to be highly upregulated in response to drought treatment for 24h and 48h. Among the highly stress-responsive MtGST members, MtGSTU17 showed strong affinity towards its conventional substrates reduced glutathione (GSH) and 1‐chloro‐2,4‐dinitrobenzene (CDNB) with the lowest binding energy of—5.7 kcal/mol and -6.5 kcal/mol, respectively. Furthermore, the substrate-binding site residues of MtGSTU17 were found to be highly conserved. These findings will facilitate the further functional and evolutionary characterization of GST genes in Medicago.

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“…Glyma01g04710 related to GST was found to be resided in the QTL qWDC1-1 . GSTs play multiple roles in plants including drought stress response in Arabidopsis [ 38 ], rice [ 39 ], and soybean [ 40 ]. Over-expression of a GST gene, GsGST , from wild soybean ( Glycine soja ) enhances drought and salt tolerance in transgenic tobacco [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Quantitative Trait Locus Mapping for Drought Tolerance in Soybean Recombinant Inbred Line Population

Dhungana

Park

et al. 2021

Plants

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Improving drought stress tolerance of soybean could be an effective way to minimize the yield reduction in the drought prevailing regions. Identification of drought tolerance-related quantitative trait loci (QTLs) is useful to facilitate the development of stress-tolerant varieties. This study aimed to identify the QTLs for drought tolerance in soybean using a recombinant inbred line (RIL) population developed from the cross between a drought-tolerant ‘PI416937’ and a susceptible ‘Cheonsang’ cultivar. Phenotyping was done with a weighted drought coefficient derived from the vegetative and reproductive traits. The genetic map was constructed using 2648 polymorphic SNP markers that distributed on 20 chromosomes with a mean genetic distance of 1.36 cM between markers. A total of 10 QTLs with 3.52–4.7 logarithm of odds value accounting for up to 12.9% phenotypic variance were identified on seven chromosomes. Five chromosomes—2, 7, 10, 14, and 20—contained one QTL each, and chromosomes 1 and 19 harbored two and three QTLs, respectively. The chromosomal locations of seven QTLs overlapped or located close to the related QTLs and/or potential candidate genes reported earlier. The QTLs and closely linked markers could be utilized in maker-assisted selection to accelerate the breeding for drought tolerance in soybean.

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Genome-wide analysis and transcript profiling identify several abiotic and biotic stress-responsive Glutathione S-transferase genes in soybean

Cited by 13 publications

References 63 publications

Genome-wide identification, evolution, and transcript profiling of Aldehyde dehydrogenase superfamily in potato during development stages and stress conditions

Genome-wide identification, evolution, and transcript profiling of Aldehyde dehydrogenase superfamily in potato during development stages and stress conditions

Genome-wide identification and expression profiling of glutathione S-transferase family under multiple abiotic and biotic stresses in Medicago truncatula L.

Quantitative Trait Locus Mapping for Drought Tolerance in Soybean Recombinant Inbred Line Population

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