Genome-wide analysis and expression profiling of the small heat shock proteins in zebrafish

Elicker, Kimberly S.; Hutson, Lara D.

doi:10.1016/j.gene.2007.08.003

Cited by 79 publications

(91 citation statements)

References 55 publications

(66 reference statements)

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“…As stated previously, the expression of OsHsp20 genes was always induced by heat treatment. Our current results were largely consistent with the results in other papers, where nine Class I OsHsp20 genes were reported to be up-regulated by heat shock in rice (Guan et al 2004), and a number of Hsp20 genes were up-regulated by heat shock in zebrafish (Kimberly and Lara 2007) and Arabidopsis (Swindell et al 2007). In our study, four OsHsp20 genes were detected to be expressed in 25°C conditions, these genes might be involved in specific housekeeping functions of rice under normal growth conditions.…”

Section: Heat Shock Responsesupporting

confidence: 93%

Comprehensive sequence and expression profile analysis of Hsp20 gene family in rice

et al. 2009

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The Hsp20 genes represent the most abundant small heat shock proteins (sHSPs) in plants. Hsp20 gene family has been shown to be involved in preventing heat shock and promoting resistance to environmental stress factors, but very little is known about this gene family in rice. Here, we report the identification and characterization of 39 OsHsp20 genes in rice, describing the gene structure, gene expression, genome localization, and phylogenetic relationship of each member. We have used RT-PCR to perform a characterization of the normal and heat shock-induced expression of selective OsHsp20 genes. A genome-wide microarray based gene expression analysis involving 25 stages of vegetative and reproductive development in three rice cultivars has revealed that 36 OsHsp20 genes were expressed in at least one of the experimental stages studied. Among these, transcripts of OsHsp20 were accumulated differentially during vegetative and reproductive developmental stages and preferentially down-regulated in Shanyou 63. In addition, OsHsp20 genes were identified as showing prominent heterosis in family-level expression. Our results suggest that the expression patterns of the OsHsp20 genes are diversified not only in developmental stages but also in variety level.

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Section: Heat Shock Responsesupporting

confidence: 93%

Comprehensive sequence and expression profile analysis of Hsp20 gene family in rice

et al. 2009

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“…1, the resulting cladogram (Fig. 2) displays a highly supported HSPC034 clade, and a separate sHSP clade that accurately presents the major relationships amongst vertebrate HSPB1-10, as earlier established independently by Fontaine et al (2003), Franck et al (2004), and Elicker and Hutson (2007). It thus shows that the sequence similarities between human HSPC034 and human HSPB1 (Hsp27), HSPB2, HSPB5, and HSPB9, as reported by Bellyei et al (2007b), do not reflect any specific evolutionary relatedness of human HSPC034 with these HSPB members.…”

mentioning

confidence: 64%

Why proteins without an α-crystallin domain should not be included in the human small heat shock protein family HSPB

Kappé

Boelens

Jong

2010

Cell Stress and Chaperones

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The presence of an alpha-crystallin domain documents the evolutionary relatedness of the ubiquitous family of small heat shock proteins. Sequence and three-dimensional structure provide no evidence for the presence of such a domain in HSPC034, recently proposed as the 11th member of the human HSPB family. Also, phylogenetic analyses detect no relationship between HSPC034 and the human HSPB1-10 sequences. Arguments are provided as to why inclusion in the HSPB family of proteins like HSPC034, which resemble small heat shock proteins in being heat-inducible and having chaperone-like properties and a low monomeric mass, but are evolutionarily unrelated, is misleading and confusing.

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“…Scale bars are 50 μm. A Western blot probed for Hsp70 and Hsp27 expression in white, or fast-twitch, muscle (wm), red, or slow-twitch, muscle (rm), brain, heart, lens, testis, and gut tissues is shown for comparison Humans and mice have genes for ten related small heat shock proteins (Fontaine et al 2003), and zebrafish have genes for 15 such proteins (Elicker and Hutson 2007). In mammals, expression of the related αB crystallin in skeletal and cardiac muscle tissues has been well documented (see Inaguma et al (1993), for example), and these proteins have some common functionality (Jakob et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Hsp27 is persistently expressed in zebrafish skeletal and cardiac muscle tissues but dispensable for their morphogenesis

Tucker

Ustyugov

Bryantsev

et al. 2009

Cell Stress and Chaperones

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Constitutive expression of Hsp27 has been demonstrated in vertebrate embryos, especially in developing skeletal and cardiac muscle. Results of several previous studies have indicated that Hsp27 could play a role in the development of these tissues. For example, inhibition of Hsp27 expression has been reported to cause defective development of mammalian myoblasts in vitro and frog embryos in vivo. In contrast, transgenic mice lacking Hsp27 develop normally. Here, we examined the distribution of Hsp27 protein in developing and adult zebrafish and effects of suppressing Hsp27 expression using phosphorodiamidate morpholino oligonucleotides (PMO) on zebrafish development. Consistent with our previous analysis of hsp27 messenger RNA expression, we detected the protein Hsp27 in cardiac, smooth, and skeletal muscle of both embryonic and adult zebrafish. However, embryos lacking detectable Hsp27 after injection of antisense hsp27 PMO exhibited comparable heart beat rates to that of control embryos and cardiac morphology was indistinguishable in the presence or absence of Hsp27. Loss of Hsp27 also had no effect on the structure of the skeletal muscle myotomes in the developing embryo. Finally, embryos injected with antisense hsp27 and scrambled control PMO displayed equal motility. We conclude that Hsp27 is dispensable for zebrafish morphogenesis but could play a role in long-term maintenance of heart and muscle tissues.

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Genome-wide analysis and expression profiling of the small heat shock proteins in zebrafish

Cited by 79 publications

References 55 publications

Comprehensive sequence and expression profile analysis of Hsp20 gene family in rice

Comprehensive sequence and expression profile analysis of Hsp20 gene family in rice

Why proteins without an α-crystallin domain should not be included in the human small heat shock protein family HSPB

Hsp27 is persistently expressed in zebrafish skeletal and cardiac muscle tissues but dispensable for their morphogenesis

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