Genome-Wide Analysis and Expression Profiling of the Phospholipase D Gene Family in Solanum tuberosum

Abstract

Phospholipase D (PLD) is the most important phospholipid hydrolase in plants, which can hydrolyze phospholipids into phosphatidic acid (PA) and choline. When plants encounter low temperature, drought and high salt stress, phospholipase D and its products play an important role in regulating plant growth and development and coping with stress. In this study, 16 members of StPLD gene family were identified in potato genome, which were distributed in α, β, δ, and ζ subfamilies, and their expression patterns under salt, high temperature, drought, and ABA stress were detected by qRT-PCR method. Gene expression analysis showed that the expression of StPLD genes in potato was upregulated and downregulated to varying degrees under the four stresses, indicating that the PLD gene family is involved in the interaction of potato plant hormones and abiotic stress signals. Chromosome distribution showed that StPLD gene was unevenly distributed on 8 chromosomes, and only one pair of tandem repeat genes was found. All StPLD promoters contain hormone and stress-related cis-regulatory elements to respond to different stresses. Structural analysis showed that StPLD genes in the same subgroup had a similar exon–intron structure. Our study provides a valuable reference for further research of the function and structure of PLD gene.

Genome-Wide Identification of LRR-RLK Family in Saccharum and Expression Analysis in Response to Biotic and Abiotic Stress

“…In addition, 22 SsLRR-RLK genes were located on Chr4D, followed by 19 on Chr1A, 2B, 2D and 4A, and the least (seven) on Chr5D and Chr7C. The results indicate that the distribution pattern of LRR-RLK in S. spontaneum is unlike the other gene family, such as the potato PLD gene family [56] and the maize JHDM gene family [34], while it is similar with LRR-RLK gene families in other plant species, such as soybeans [11].…”

Genome-Wide Investigation of the PLD Gene Family in Tomato: Identification, Analysis, and Expression

“…The PLD gene family gene structures of tomato and potato are similar; the gene length of subtype α is short with fewer exons, while the gene length of subtype δ is short with more exons. The subtype β consists of three members, while the subtype ζ has two members and the gene lengths are longer than other subtypes [ 14 ].…”

“…In 1994, the first PLD gene was cloned in Ricinus communis [ 8 ]. To date, the genome-wide identification of PLD genes has been achieved in various crops, including 12 genes in Arabidopsis thaliana , 17 in Oryza sativa , 18 in Glycine max , 22 in maize ( Zea mays ), 32 in Brassica napus , 16 in Solanum tuberosum , 13 in Sorghum bicolor , and 59 in Medicago sativa [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ]. In the dicotyledon A. thaliana , the 12 PLD genes are classified into six subtypes, namely, α, β, γ, δ, ζ, and ε [ 17 ].…”

“…An increase in the expression of AtPLDα in Arabidopsis enhances stomatal closure [ 26 ]. In potato ( S. tuberosum ), StPLDα5 is specifically expressed in the stamens, and StPLDα5 is associated with tolerance to salt, drought, and heat [ 14 ]. The expression of OsPLDβ1 promotes ABA signaling by activating SAPK, thereby negatively affecting seed germination [ 10 ].…”

Characterization and evolutionary diversification of the phospholipase D gene family in mosses

“…In addition to those of A. thaliana and O. sativa , PLDs in various angiosperms have been investigated . For example, 10 and 16 PLDs were found in the genome of pineapple ( Ananas comosus ) and potato ( Solanum tuberosum ), respectively ( Hong et al, 2017 ; Li et al, 2021 ). When treated with hexaldehyde, the expression of PLD2 in pineapple fruit was upregulated ( Hong et al, 2017 ).…”