Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.)

Abstract

Heat shock proteins (HSPs) perform a fundamental role in protecting plants against abiotic stresses. Previous studies have made great efforts in the functional analysis of individual family members, but there has not yet been an overall analysis or expression profiling of the HSP70 gene family in soybeans (Glycine max L.). In this study, an investigation of the soybean genome revealed 61 putative HSP70 genes, which were evaluated. These genes were classified into eight sub-families, denoted I–VIII, based on a phylogenetic analysis. In each sub-family, the constituent parts of the gene structure and motif were relatively conserved. These GmHSP70 genes were distributed unequally on 17 of the 20 chromosomes. The analysis of the expression profiles showed that 53 of the 61 GmHSP70 genes were differentially expressed across the 14 tissues. However, most of the GmHSP70s were differentially expressed in a tissue-specific expression pattern. Furthermore, the expression of some of the duplicate genes was partially redundant, while others showed functional diversity. The quantitative real-time PCR (qRT-PCR) analysis of the 61 soybean HSP70 genes confirmed their stress-inducible expression patterns under both drought and heat stress. These findings provide a thorough overview of the evolution and modification of the GmHSP70 gene family, which will help to determine the functional characteristics of the HSP70 genes in soybean growth and development.

Label‐free quantitative proteomic analysis of tolerance to drought in Pisum sativum

“…HSPs reveal a specific role in protein folding (chaperone function) and protection of nascent proteins during their transport into specific organelles such as plastids and mitochondria . In addition play important roles in different kinds of environmental stress conditions, such as heat and drought, low, and high temperatures and salinity . Several studies demonstrated an increase of plant HSP under different stress conditions, indicating an enhanced need for protein protection under drought stress .…”

Genome-Wide Identification, Classification, and Expression Analysis of Amino Acid Transporter Gene Family in Glycine Max

“…It suggested the WGD duplication might be the main mechanism of AAT gene family expansion and functional diversity during evolution of soybean. This result consisted with some other gene families in soybean, such as GST supergene family (Liu et al, 2015 ), receptor-like protein kinase genes (Zhou et al, 2016 ), HSP70 genes (Zhang et al, 2015 ) in soybean, or AP2/ERF superfamily genes and 14-3-3 family genes in Medicago truncatula (Chen et al, 2001 ; Qin et al, 2016 ; Shu et al, 2016 ).…”

Genetics and Molecular Mapping of Heat Tolerance for Seedling Survival and Pod Set in Lentil

“…The existence of signal molecules such as mitogen-activated protein kinases (MAPKs) and dehydration-responsive elements (DREs) that can switch on and off a battery of genes in response to heat stress has been reported (Sangwan et al, 2002;Kidokoro et al, 2015). Further, role of heat shock proteins in the maintenance of protein homeostasis within plants in response to heat stress also suggests the trait's monogenic nature (Zhang et al, 2015). The monogenic nature of a complex trait has also been reported for other abiotic stresses (i.e., drought tolerance in lentil) (Idrissi et al, 2016;Singh et al, 2016a).…”