Genome type analysis of Brazilian adenovirus strains of serotypes 1, 2, 3, 5, and 7 collected between 1976 and 1995

Abstract

A collection of 92 epidemiologically unrelated isolates of Ad1 (n = 14), Ad2 (n = 29), Ad3 (n = 19), Ad5 (n = 16), and Ad7 (n = 14) collected in the cities of Belem do Pará (1 degree S 48 degrees W) and Rio de Janeiro (23 degrees S 43 degrees W) between 1976 and 1995 from patients with respiratory disease and conjunctivitis were characterized by restriction enzyme analysis of genomic DNA. Among the strains of subgenus B, two different genome types of serotype 7, 7b and 7e, were identified. The analysis of their temporal distribution throughout the study period suggested an alternating appearance of these two DNA variants. Only one genome type of Ad3, 3p, was detected during the sampling period. Further analysis with Xba I, Bcl I, and Hpa I indicated that it is a p1-like genome type. Both previously described and new genomic variants were identified among subgenus C strains. Genome types D1, D7, D10, and one not previously described were identified among the 14 Ad1 strains analyzed. Genome types D2, D5, D25, and 13 new DNA variants were identified among the 29 Ad2 isolates. Genome type D38 and 5 new variants were found among the 16 strains of Ad5. In spite of the relatively small size of the sample analyzed, the results of this study confirm the important genetic variability previously observed for members of subgenus C by other authors.

Antigenic and genomic characterization of adenovirus associated to respiratory infections in children living in Northeast Brazil

“…These studies have shown an important genetic variability for members of subgenus C. Among members of subgenus B, serotype 3 has been represented by genotypes 3p1 and 3a variant described previously (Kajon et al 1999, Moura et al 2007. Data of Brazilian studies suggest replacements of Ad7 genome types circulating in Brazil as has been reported in other studies (Kajon & Wadell 1994, Erdman et al 2000.…”

“…The data obtained in this study show that genotypes circulating in Salvador during 1998 had circulated in other Brazilian cities and other South American countries in previous years. The genotypes D1 and D10 of Ad1, and the genotype D5 of Ad2 identified in Salvador during 1998 had circulated in the cities of Belém and Rio de Janeiro in the 80s and early 90s (Kajon et al 1999). The only Ad5 detected in Salvador had circulated in Argentina between 1984and 1988(Kajon & Wadell 1992.…”

“…This finding differs from data reported by other Brazilian and South American studies, which indicate Ad as the second most prevalent virus in ARI observed in hospitalized children (Nascimento et al 1991, Kajon et al 1999, Larrañaga et al 2000, Straliotto et al 2002. In this study the rate of ARI by Ad in hospitalized patients was 1.6%, while in other studies this rate ranged from 3.3% to 12.6% (Larrañaga et al 2000, Hong et al 2001, Tsai et al 2001, Chen et al 2004.…”

“…In spite of the small number of Ad analyzed, the results of this study show a relative genetic variability for members of species C, as observed previously (Kajon et al 1999, Frabasile et al 2005, Moura et al 2007. Several studies have shown that one particular genotype can circulate in different geographic regions in different years.…”

“…Several reports of the genomic characterization of Brazilian Ad isolates from patients with respiratory, ocular and gastrointestinal infections have been published since 1988, but none of them included any Ad isolates of patients living in Northeast Brazil (Wadell et al 1985, Gomes et al 1988, Moraes et al 1997, Kajon et al 1999, de Albuquerque et al 2003, Moura et al 2007). These studies have shown an important genetic variability for members of subgenus C. Among members of subgenus B, serotype 3 has been represented by genotypes 3p1 and 3a variant described previously (Kajon et al 1999, Moura et al 2007.…”