Genome Stability in Caenorhabditis elegans

Rieckher, Matthias; Lopes, Amanda Franqueira Cardoso; Schumacher, Björn

doi:10.1016/b978-0-12-803309-8.00011-2

Cited by 2 publications

(4 citation statements)

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“…Animals are easy to maintain, the genome is fully sequenced and annotated, and there is an array of methods for genetic manipulation, including RNAi, transgenesis, genome editing, and random mutagenesis, which allow for a comparably simple design of forward and reverse genetic screens. Importantly, nematodes are susceptible to a variety of DNA damaging agents and most DNA repair mechanism are highly conserved (summarized in (Rieckher et al, )). Developmental timing of C. elegans is highly reproducible: a full life cycle requires 2.5 days at 20°C during which animals develop from the fertilized oocyte to embryos, followed by the four larval stages (L1–L4) and finalized as adult hermaphrodite that produces 300 eggs by self‐fertilization (Figure a).…”

Section: Introductionmentioning

confidence: 99%

“…Importantly, nematodes are susceptible to a variety of DNA damaging agents and most DNA repair mechanism are highly conserved (summarized in (Rieckher et al, 2016)). Developmental timing of C. elegans is highly reproducible: a full life cycle requires 2.5 days at 20°C during which animals develop from the fertilized oocyte to embryos, followed by the four larval stages (L1-L4) and finalized as adult hermaphrodite that produces 300 eggs by self-fertilization ( Figure 1a).…”

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confidence: 99%

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A simple answer to complex questions: Caenorhabditis elegans as an experimental model for examining the DNA damage response and disease genes

Rieckher

Bujarrabal

Doll

et al. 2017

Journal Cellular Physiology

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The genetic information is constantly challenged by genotoxic attacks. DNA repair mechanisms evolved early in evolution and recognize and remove the various lesions. A complex network of DNA damage responses (DDR) orchestrates a variety of physiological adaptations to the presence of genome instability. Erroneous repair or malfunctioning of the DDR causes cancer development and the accumulation of DNA lesions drives the aging process. For understanding the complex DNA repair and DDR mechanisms it is pivotal to employ simple metazoan as model systems. The nematode Caenorhabditis elegans has become a well-established and popular experimental organism that allows dissecting genome stability mechanisms in dynamic and differentiated tissues and under physiological conditions. We provide an overview of the distinct advantages of the nematode system for studying DDR and provide a range of currently applied methodologies.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

A simple answer to complex questions: Caenorhabditis elegans as an experimental model for examining the DNA damage response and disease genes

Rieckher

Bujarrabal

Doll

et al. 2017

Journal Cellular Physiology

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“…Defects in genomic stability often lead to fertility deficiencies in animals [39][40][41]. As described in previous studies, the loss of the SMC-5/6 complex in C. elegans led to a reduction in brood-size as compared with the wild-type [25,54].…”

Section: Discussionmentioning

confidence: 58%

“…The DNA damage from genotoxic insults should be repaired by the cell. However, if left unrepaired, damaged cells either become apoptotic corpses or dead eggs after fertilization, resulting in reduced progeny survival [29,[39][40][41]. To investigate the importance of nse-4 in the repair competency, L1 worms were treated with MMS for 16 h, recovered for 72 h, and thereafter the viability of the eggs laid was scored [31,32].…”

Section: Nse-4 Is Required For Efficient Dna Repair In Germ Cellsmentioning

confidence: 99%

Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans

Odiba

Ezechukwu

Liao

et al. 2022

IJMS

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The Structural Maintenance of Chromosomes (SMC) complex plays an important role in maintaining chromosome integrity, in which the SMC5/6 complex occupies a central position by facilitating mitotic and meiotic processes as well as DNA repair. NSE-4 Kleisin is critical for both the organization and function of the SMC5/6 complex, bridging NSE1 and NSE3 (MAGE related) with the head domains of the SMC5 and SMC6 proteins. Despite the conservation in protein sequence, no functional relevance of the NSE-4 homologous protein (NSE-4) in Caenorhabditis elegans has been reported. Here, we demonstrated the essential role of C. elegans NSE-4 in genome maintenance and DNA repair. Our results showed that NSE-4 is essential for the maintenance of chromosomal structure and repair of a range of chemically induced DNA damage. Furthermore, NSE-4 is involved in inter-sister repair during meiosis. NSE-4 localizes on the chromosome and is indispensable for the localization of NSE-1. Collectively, our data from this study provide further insight into the evolutionary conservation and diversification of NSE-4 function in the SMC-5/6 complex.

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Genome Stability in Caenorhabditis elegans

Cited by 2 publications

References 203 publications

A simple answer to complex questions: Caenorhabditis elegans as an experimental model for examining the DNA damage response and disease genes

A simple answer to complex questions: Caenorhabditis elegans as an experimental model for examining the DNA damage response and disease genes

Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans

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