Genome size and identification of repetitive DNA sequences using low coverage sequencing in Hancornia speciosa Gomes (Apocynaceae: Gentianales)

DNA Origami-Enabled Gene Localization of Repetitive Sequences

“…Organisms exhibit a wide range of traits determined by thousands of genes that are clustered on chromosomes carrying genetic information. Acquired and congenital gene variations (e.g., single nucleotide polymorphisms, complex gene structural variation) will threaten genomic stability, which eventually leads to genetic diseases. − Among them, the abnormal expansion of DNA repetitive sequences can cause more than 50 intractable human diseases (e.g., frontotemporal dementia, Huntington’s disease). , Typical methods for the detection of DNA repetitive sequences are sequencing and optical imaging-based technologies. − In 1990, Human Genome Project was implemented, aiming to determine the DNA sequence involved in the human chromosome and map the human genome for an in-depth understanding of gene localization and function. − This project has revolutionized sequencing technologies, which gives rise to the emergence of diverse methods for gene localization, such as Sanger sequencing technology, − Illumina sequencing technology, − and nanopore sequencing technology. − Each of these sequencing technologies has its application scenarios because of its own characteristics: Sanger sequencing for short read lengths, high accuracy, reliability, and controllability; Illumina sequencing for short read lengths, high throughput, high sensitivity, and suitability for large-scale sequencing; and nanopore sequencing for long read lengths, real-time sequencing, and portability. − …”