Genome size analysis of field grown and somatic embryo regenerated plants in Allium sativum L.

Malik, Moien Qadir; Mujib, A.; Gulzar, Basit; Zafar, Nadia; Syeed, Rukaya; Mamgain, Jyoti; Ejaz, Bushra

doi:10.1007/s13353-019-00536-5

Cited by 27 publications

(10 citation statements)

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“…Thus, the ploidy level and the genetic homogeneity were maintained which is in line with Niazian et al ( 2017 ) observation, showing the genetic stability of regenerated plants in Carum copticum . Recently, similar genome size stability was reported in micropropagated plants of Gladiolus (Mujib et al 2017 ), Rauvolfia serpentina (Zafar et al 2019 ), Allium sativum (Malik et al 2020a ) and species of Zepheranthes (Syeed et al 2021 ). The alteration of genetic complement was however, reported in other investigated plants like Elaeis guineensis and Cucumis melo of tissue culture origin (Giorgetti et al 2011 ; Raji and Farajpore 2020 ).…”

Section: Discussionsupporting

confidence: 53%

Flow cytometry and start codon targeted (SCoT) genetic fidelity assessment of regenerated plantlets in Tylophora indica (Burm.f.) Merrill

Mamgain¹,

Mujib²,

Ejaz³

et al. 2022

Plant Cell Tiss Organ Cult

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Tylophora indica (Burm.f.) Merrill. is a pharmacologically important plant, popular for alkaloidal and non-alkaloidal richness. Large scale propagation of T. indica is difficult in the wild as the seeds are small and the frequency of germination is very poor. In the present study, the genome size estimation of in vitro regenerated (indirect, direct and somatic embryo mediated) T. indica was made by flow cytometric method. Clonal fidelity of the regenerants was assessed using a start codon targeted (SCoT) molecular marker. Initially, the explants were inoculated on Murashige and Skoog basal medium supplemented with various concentrations of plant growth regulators like 2,4-dichlorophenoxy acetic acid (2,4-D), Kinetin, 6-benzyl amino purine (BAP) and 1-naphthalene acetic acid either singly or in combinations. The highest callus induction frequency (87.75%) was obtained in 6.7 µM 2,4-D added MS medium which metamorphosed into progressive stages (globular, heart, torpedo, and cotyledonary) of embryos. Mature and healthy somatic embryos efficiently germinated into plantlets on 8.8 µM BAP + 1.4 µM GA 3 enriched MS medium. Histological and scanning electron microscopic study confirmed the above developing stages . The regenerated shoots were rooted best in 2.45 µM Indole-3-butyric acid supplemented solid MS medium. The plants were hardened and acclimatized with 90% survivability. The flow cytometric 2C DNA content of indirect, direct and somatic embryo derived plants was 1.896 pg, 1.940 pg and 1.926 pg respectively, very similar to the mother plant (1.928 pg). SCoT marker generated a high percentage of monomorphic bands (94%) revealing similarity with the mother plant, thus ensuring genetic fidelity. To the best of our knowledge, this is perhaps the first ever report of 2C DNA content estimation and SCoT marker based genetic homogeneity study in T. indica . Supplementary Information The online version contains supplementary material available at 10.1007/s11240-022-02254-z.

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Section: Discussionsupporting

confidence: 53%

Flow cytometry and start codon targeted (SCoT) genetic fidelity assessment of regenerated plantlets in Tylophora indica (Burm.f.) Merrill

Mamgain¹,

Mujib²,

Ejaz³

et al. 2022

Plant Cell Tiss Organ Cult

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“…In contrast, Park et al (2011) found that the embryogenic capacity and DNA content in the nucleus decreased during prolonged maintenance. In general, the genotype has to be carefully chosen because not all ETs grow well in culture (Montalban et al 2012;Zhang et al 2014;Malik et al 2020). In addition, whether the maturation of SEs is correlated with morphological development and intracellular communication has also been considered (Godel-Jedrychowska et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of somatic embryo production during embryogenic tissue proliferation stage using morphology, maternal genotype, proliferation rate and tissue age of Pinus thunbergii Parl

et al. 2021

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To determine the optimal embryogenic capacity (somatic embryo production) of the selected elite nematode-resistant genotypes of Pinus thunbergii, variables such as embryogenic tissue (ET) morphology, maternal genotype, proliferation rate and tissue age were analyzed. ET morphology and histological evaluation of the proliferation stage showed a decrease in filamentous clump and protuberant surfaces and a decline in the acetocarmine-staining area, which indicates a decrease in somatic embryo production (SEP). Variations in cell physiology during the proliferation stage showed that SEP was positively correlated with soluble sugars and proteins, but negatively correlated with starch, peroxidase, and superoxidase. In addition, SEP was significantly (p < 0.001) affected by maternal genotype, tissue age and proliferation rate. Moreover, SEP was positively correlated with proliferation rate (r = 0.98, p < 0.001), but negatively correlated with tissue age (r = − 0.95, p < 0.001). In general, the results suggest that SEP could be assessed in ET proliferation stages by the apparent cell morphology, histology, proliferation rate and tissue age, which provides novel insights for evaluating the ET maturation capacity (number of somatic embryos) during the proliferation stage of P. thunbergii somatic embryogenesis.

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“…Allium sativum L. is a fragrant herbaceous plant that grows up to 30 to 80cm tall (Sendl., 1995). A. sativum is thought to have evolved in Central Asia 600 years ago and has subsequently expanded over Southwest Asia and the Mediterranean (Malik et al, 2020). A. sativum was tested against larvae of Anopheles stephensi and the LC 50 values for hexane extract were 7.5 ppm after 24 hours and 7.6 ppm after 48 hours of exposure.…”

Section: Liliaceaementioning

confidence: 99%

Prospective Plants with Corroborated Antimalarial Actions: A Review

et al. 2021

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Malaria is a serious illness resulted from parasites that are communicated to people through the bites of infected female Anopheles mosquitoes. Malaria is still in a worrying trend, particularly in tropical and subtropical climates although it is curable and preventable. In spite of a noteworthy abetment in incidence and death rates caused by malaria, even in 2017, a big number of people (219 million) have been affected by it along with 435 thousand confirmed death cases. Though a lot of synthetic drugs have been commercialized to treat malaria, those are compromised with some serious side effects. On the contrary, plant sources are always getting a big focus to develop novel and effective therapeutics in the treatment of different ailments i.e. quinine and artemisinin to treat malarial complications. The usage of herbal plants against malaria has also a very ancient root. Several families of plant species have showed potential antimalarial activities in previous research works. In this review work, families of these plants have been compiled so that prospective researchers can find a hint to discover more effective and safer plant-derived therapeutic options against malaria. Bangladesh Pharmaceutical Journal 24(2): 180-193, 2021

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Genome size analysis of field grown and somatic embryo regenerated plants in Allium sativum L.

Cited by 27 publications

References 53 publications

Flow cytometry and start codon targeted (SCoT) genetic fidelity assessment of regenerated plantlets in Tylophora indica (Burm.f.) Merrill

Flow cytometry and start codon targeted (SCoT) genetic fidelity assessment of regenerated plantlets in Tylophora indica (Burm.f.) Merrill

Evaluation of somatic embryo production during embryogenic tissue proliferation stage using morphology, maternal genotype, proliferation rate and tissue age of Pinus thunbergii Parl

Prospective Plants with Corroborated Antimalarial Actions: A Review

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