Genome shuffling of Lactobacillus delbrueckii mutant and Bacillus amyloliquefaciens through protoplasmic fusion for l-lactic acid production from starchy wastes

Genetically Engineered Lactobacilli for Technological and Functional Food Applications

“…Shuffled L. rhamnosus strains with improved tolerance to glucose and enhanced Llactate production has also been obtained (Yasuda et al, 2008). In the same way, a fusant derived from Lactobacillus delbueckii able of growing at low pH and utilizing starch from cassava bagasse was obtained and it produced large amounts of L-lactic (John et al, 2008).…”

Strategies of Strain Improvement of Industrial Microbes

“…It has been commercially used for the production of chirally pure side chain of captopril (Elander 1995 ). Genencor and DuPont have carried out collaborative project for the development of an economical process of conversion of glucose into 1, 3-propanediol using recombinant E. coli which consists of two metabolic pathways for conversion of glucose Non-fastidious strain obtained after three rounds of genome shuffl ing for direct conversion of starch to lactic acid John et al ( 2008 ) Sphingobium chlorophenolicum Higher tolerance level of pentachlorophenol for degradation at a faster rate Dai and Copley ( 2004 ) Strain tolerance to end product…”

High-Yield Laccase-Producing Strains Constructed by Protoplast Fusion Between Bacterium and Fungus

“…B is colony number of the solid PDA (the cells with enzymetreatment were washed twice with distilled water to lyse protoplasts and spread onto the solid PDA) [13]; C is colony number of the regeneration medium plates (protoplasts from different populations were mixed and spread on the regeneration medium plates and were also incubated) [14].…”