Genome sequencing of a yeast-like fungal strain P6, a novel species of Aureobasidium spp.: insights into its taxonomy, evolution, and biotechnological potentials

Abstract

Purpose This study aimed to look insights into taxonomy, evolution, and biotechnological potentials of a yeast-like fungal strain P6 isolated from a mangrove ecosystem. Methods The genome sequencing for the yeast-like fungal strain P6 was conducted on a Hiseq sequencing platform, and the genomic characteristics and annotations were analyzed. The central metabolism and gluconate biosynthesis pathway were studied through the genome sequence data by using the GO, KOG, and KEGG databases. The secondary metabolite potentials were also evaluated. Results The whole genome size of the P6 strain was 25.41Mb and the G + C content of its genome was 50.69%. Totally, 6098 protein-coding genes and 264 non-coding RNA genes were predicted. The annotation results showed that the yeast-like fungal strain P6 had complete metabolic pathways of TCA cycle, EMP pathway, pentose phosphate pathway, glyoxylic acid cycle, and other central metabolic pathways. Furthermore, the inulinase activity associated with β-fructofuranosidase and high glucose oxidase activity in this strain have been demonstrated. It was found that this yeast-like fungal strain was located at root of most species of Aureobasidium spp. and at a separate cluster of all the phylogenetic trees. The P6 strain was predicted to contain three NRPS gene clusters, five type-I PKS gene clusters, and one type-I NRPS/PKS gene cluster via analysis at the antiSMASH Website. It may synthesize epichloenin A, fusaric acid, elsinochromes, and fusaridione A. Conclusions Based on its unique DNA sequence, taxonomic position in the phylogenetic tree and evolutional position, the yeast-like fungal strain P6 was identified as a novel species Aureobasidium hainanensis sp. nov. P6 isolate and had highly potential applications.

Transcription Factors in Aureobasidium spp.: Classification, Regulation and a Newly Built Database

“…and Aspergillus spp. at ancient times [ 18 ]. For tuning iron uptake and storage of Aureobasidium melanogenum HN6.2, the transcriptome analysis demonstrates that the zinc cluster transcription factor SreA regulates and maintains iron homeostasis ( Table S2 ) [ 23 ].…”

“…All the TFs are annotated in the GhostKOALA, the Cluster of Orthologous Groups of proteins (COG), Pfam, the Non-Redundant Protein Sequence Database (NR) and the Swiss-Prot database as previously described [ 18 , 19 , 20 ]. Each of them is summarized in reference to the YEASTRACT+ database and the published references for inferring the functions.…”

Use of Aureobasidium in a sustainable economy

“…Currently, 32 DNA-identified Aureobasidium species are known (Table 1 ). These species include the best known Aureobasidium species, Aureobasidium pullulans, as well as the most recently identified species Aureobasidium insectorum , Aureobasidium planticola , Aureobasidium motuoense , and Aureobasidium intercalariosporum (Arnaud 1918 ; Arzanlou and Khodaei 2012 ; Ashish and Pratibha 2018 ; Barr 2001 ; Bills et al 2012 ; Ciferri et al 1957 ; Cooke 1962 ; Crous et al 2021 , 2011 ; de Hoog and Hermanides-Nijhof 1977a , 1977b ; Gostinčar et al 2014 ; Inamdar et al 2019 ; Jia et al 2019 ; Jiang et al 2021 , 2019 ; Lee et al 2021 ; Nasr et al 2018 ; Onetto et al 2020 ; Peterson et al 2013 ; Ramaley 1992 ; Wang et al 2022a ; Wu et al 2023 ). An additional 15 species have been identified based on morphology (Table 2 ) (Cooke 1962 ; Crisan and Hodisan 1964 ; Della Torre 1963 ; de Hoog and Hermanides-Nijhof 1977a ; Pande and Ghate 1985 ; Richardson and Pitkäranta 2011 ).…”

Biosynthetic Polymalic Acid as a Delivery Nanoplatform for Translational Cancer Medicine

“…In one variety of A. pullulan , namely Aureobasidium melanogenum , a characteristic NRSP was recently reported to be a putative PMLA synthetase that contains an adenylation domain for ATP binding and malyl-AMP formation, an activatable thiolation domain for phosphopantetheine attachment and polymerization of malyl-AMP into PMLA, and a hexa-transmembrane region for transport of PMLA out of the cytoplasm [ 41 ]. Because the whole-genome sequences of several PMLA-producing fungal strains have been determined, a conserved PMLA synthetase across species is expected to be unmasked in the near future [ 22 , 42 ].…”