Genome sequencing and analysis of a novel recombinant porcine epidemic diarrhea virus strain from Henan, China

Li, Renfeng; Qiao, Songlin; Yu, Yang; Guo, Junqing; Xie, Sha; Zhou, En‐Min; Zhang, Gaiping

doi:10.1007/s11262-015-1254-1

Cited by 57 publications

(47 citation statements)

References 31 publications

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“…Recombination and insertions and deletions have repeatedly been demonstrated in PEDV by genome analyses of isolates during more recent outbreaks in Asia and in the USA (Fan et al, 2012;Li et al, 2016;Jarvis et al, 2016;Vlasova et al, 2014;Oka et al, 2014).…”

Section: The Pedv After Its First Detectionmentioning

confidence: 92%

Porcine epidemic diarrhea: A retrospect from Europe and matters of debate

Pensaert

Martelli

2016

Virus Research

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A retrospect is given on the emergence of porcine epidemic diarrhea (PED) during the early seventies in Europe. While, at first, it appeared as a disease affecting feeder pigs, fattening- and adult swine, it later also became pathogenic for neonatal and suckling pigs hereby drastically increasing its economic impact. Isolation of the causative virus revealed a new porcine coronavirus, the origin of which has never been clarified. Pathogenesis studies with the prototype strain CV777 showed severe villous atrophy in neonatal pigs and the virus-animal interactions showed many similarities with transmissible gastro-enteritis virus (TGEV), another porcine coronavirus. Disease patterns in field outbreaks showed muchvariation but, while farm related factors played a role, possible genetic variations of virus strains in Europe have not been examined and are thus unknown. CV777 in experimental pigs caused diarrheal disease and mortality rates similar to those later encountered in Asia and more recently with the "original" US strains even though genomic typing of the prototype European strain have shown that it belongs to the S-INDEL strains. In Europe, PED has become endemic during the eighties and nineties and subsequently regressed so that, after 2000, swine populations in many countries have largely become seronegative. Sporadic outbreaks have recently reappeared showing a large variety of clinical outcomes.

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Section: The Pedv After Its First Detectionmentioning

confidence: 92%

Porcine epidemic diarrhea: A retrospect from Europe and matters of debate

Pensaert

Martelli

2016

Virus Research

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“…A further investigation demonstrated that the recombination of this PEDV strain mainly occurred between classical and variant strains in the S gene, suggesting that recombination occurred in the S gene of Chinese PEDV strains (unpublished data). Li et al (2016) also isolated a recombinant PEDV, designated CH/HNQX-3/14, and a recombinant analysis showed that CH/HNQX-3/14 arose from the recombination of an attenuated vaccine strain (CV777 or DR13) and a pandemic variant (CH/ZMDZY/11). This recombination occurred not only in the structural-protein-coding region (S and N genes), but also in the nonstructural-protein-coding region (replicase 1a and ORF3) .…”

Section: Emergence Of Recombinant Pedv In Chinamentioning

confidence: 99%

Porcine epidemic diarrhea in China

2016

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Porcine epidemic diarrhea (PED) is a contagious intestinal disease caused by Porcine epidemic diarrhea virus (PEDV) that characterized by vomiting, diarrhea, and dehydration. PEDV was first identified in the 1980s in China, and since then, it has become one of the most common viral causes of diarrhea. In October 2010, a large-scale outbreak of PED caused by a PEDV variant occurred in China, resulting in tremendous economic losses. This review presents a comprehensive description of PEDV history, prevalence, molecular features, and prevention and control strategies in China.

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“…However, piglets inoculated with strain KPEDV-9, which was serially passaged in Vero cells, showed reduced disease and fewer lesions (Kweon et al, 1999). Different ORF3 variants could serve as markers of vaccine-and wildtype PEDVs, and they could be a valuable tool for studying the molecular epidemiology of PEDV (Kim et al, 2016;Li et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Chinese porcine epidemic diarrhea virus with novel mutations and deletions in the S gene

Sun

Shao

et al. 2018

Veterinary Microbiology

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Porcine epidemic diarrhea (PEDV) has raised growing concerns in the pig-breeding industry because it has caused significant economic losses. To better understand the molecular epidemiology and genetic diversity of PEDV field isolates, in this study, the complete spike (S) and ORF3 genes of 17 PEDV variants in Zhejiang, China during 2014 to 2017, were characterized and analyzed. Phylogenetic analysis based on the S gene and ORF3 gene of these 17 novel PEDV strains and PEDV reference strains indicated that all the PEDV strains fell into two groups designated G1 and G2. Notably, the strains identified in 2014-2015 were in G2, while the other five strains identified from 2016 to 2017 were in G1. Sequencing and phylogenetic analyses showed that recently prevalent Chinese PEDV field strains shared higher identities with United States strains than with South Korean strains. Compared with classical vaccine strains, a series of deletions and frequently occurring mutations were observed in the receptor binding domains of our PEDV strains. Besides, we successfully isolated and reported the genetic characterization two novel PEDV strains, PEDV-LA1 and PEDV-LY4-98, found on the Chinese mainland, which had significant variations in the S gene. Meanwhile, the virulence of the new mutants may be changed, the PEDV-LY4-98 strain, which has multiple mutations in the signal peptide-encoding fragment of the S gene showed delayed cytopathic effects and smaller plaque size compared with strain PEDV-LA1, which lacks these mutations. Three unique amino acid substitutions (L7, G8, and V9) were identified in the SP-encoding fragment of the S1 N-terminal domain of the PEDV-LY4-98 S protein compared with the S proteins of all the previous PEDV strains. The animal experiment revealed that these two novel strains were high pathogenic to neonatal pigs. Whether these amino acids substitutions and the N-glycosylation site substitutions influence the antigenicity and pathogenicity of PEDV remains to be investigated. Meanwhile, amino acid substitutions in the neutralizing epitopes may have conferred the capacity for immune evasion in these PEDV field strains. This study improves our understanding of ongoing PEDV outbreaks in China, and it will guide further efforts to develop effective measures to control this virus.

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Genome sequencing and analysis of a novel recombinant porcine epidemic diarrhea virus strain from Henan, China

Cited by 57 publications

References 31 publications

Porcine epidemic diarrhea: A retrospect from Europe and matters of debate

Porcine epidemic diarrhea: A retrospect from Europe and matters of debate

Porcine epidemic diarrhea in China

Isolation and characterization of Chinese porcine epidemic diarrhea virus with novel mutations and deletions in the S gene

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