Genome sequencing and analysis of Alcaligenes faecalis subsp. phenolicus MB207

Abstract

Bacteria within the genus Alcaligenes, exhibit diverse properties but remain largely unexplored at genome scale. To shed light on the genome structure, heterogeneity and traits of Alcaligenes species, the genome of a tannery effluent isolated Alcaligenes faecalis subsp. phenolicus MB207 was sequenced and assembled. The genome was compared to the whole genome sequences of genus Alcaligenes present in the National Centre for Biotechnology Information database. Core, pan and species specific gene sequences i.e. singletons were identified. Members of this genus did not portray exceptional genetic heterogeneity or conservation and out of 5,166 protein coding genes from pooled genome dataset, 2429 (47.01%) contributed to the core, 1193 (23.09%) to singletons and 1544 (29.88%) to accessory genome. Secondary metabolite forming apparatus, antibiotic production and resistance was also profiled. Alcaligenes faecalis subsp. phenolicus MB207 genome consisted of a copious amount of bioremediation genes i.e. metal tolerance and xenobiotic degrading genes. This study marks this strain as a prospective eco-friendly bacterium with numerous benefits for the environment related research. Availability of the whole genome sequence heralds an opportunity for researchers to explore enzymes and apparatus for sustainable environmental clean-up as well as important compounds/substance production.

Complete genome sequence and analysis of Alcaligenes faecalis strain Mc250, a new potential plant bioinoculant

“…Several Af genomes have been sequenced [11,[13][14][15][16][17][18]. However, the first comparative analysis of Af genomes was published only recently, focusing on the analysis of systems related to antibiotic, metal, and pollutant resistance [13].…”

Povećanje učinkovitosti bioremedijacije na razini gena

“…Time se dobiva informacija o mehanizmu prilikom obrane, prilagodbi na onečišćeno okruženje i mehanizmu razgradnje onečišćujuće tvari. 35,36 4. Potraga za biološkim izvorima koji se mogu upotrijebiti u bioremedijaciji…”

Genomic and Physiological Traits of the Marine Bacterium Alcaligenes aquatilis QD168 Isolated From Quintero Bay, Central Chile, Reveal a Robust Adaptive Response to Environmental Stressors

“…Strains of Alcaligenes spp. have been isolated from diverse environments, such as water (Regar et al, 2016), agricultural soil (Liu et al, 2016), crude oil-polluted sites (Singha et al, 2017), industrial processes (Qiu et al, 2017; Basharat et al, 2018), insects (Quiroz-Castañeda et al, 2015) and human clinical samples (Winslow et al, 1920; Al Laham et al, 2017). Strains of Alcaligenes spp.…”

“…4HAP) and phenols ( A. faecalis subsp. phenolicus DSM 16503 T and A. faecalis JF339228) (Kiyohara et al, 1982; Hopper and Kaderbhai, 1999; Rehfuss and Urban, 2005; Adebusoye et al, 2007; Kumar et al, 2013; Sagarkar et al, 2014; Regar et al, 2016; Singha et al, 2017; Basharat et al, 2018).…”

“…phenolicus MB207 and 10 other strains of Alcaligenes spp. identified biosynthetic clusters for butyrolactone, ectoine, resorcinol, terpene, non-ribosomal peptides and polyketide synthases (Basharat et al, 2018). Strain MB207 is able to degrade azo dyes and possesses tolerance to heavy metals, although, its genomic and physiological stress determinants have not been analyzed.…”