Genome sequencing and analysis of the first spontaneous Nanosilver resistant bacterium<i>Proteus mirabilis</i>strain SCDR1

Saeb, Amr T. M.; Al‐Rubeaan, Khalid; Abouelhoda, Mohamed; Selvaraju, Manojkumar; Tayeb, Hamsa T.

doi:10.1101/089961

Cited by 4 publications

(5 citation statements)

References 63 publications

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“…Previous reports on antimicrobial susceptibility tests on P. mirabilis strains have reported that P. mirabilis strains are naturally susceptible to Rifamycin and Fusidic acid (Armbruster et al, 2018). But due to the presence of AMR genes, P. mirabilis has acquired the resistance against these antibiotics (Saeb, Al‐Rubeaan, Abouelhoda, Selvaraju, & Tayeb, 2017; Sohail, Khurshid, Murtaza Saleem, Javed, & Khan, 2015). We analyzed the drug resistance patterns of each P. mirabilis strain included in our study using ResFinder.…”

Section: Resultsmentioning

confidence: 99%

Gene interaction network approach to elucidate the multidrug resistance mechanisms in the pathogenic bacterial strain Proteus mirabilis

Miryala

Anbarasu

Ramaiah

2020

Journal Cellular Physiology

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Proteus mirabilis is one among the most frequently identified pathogen in patients with the urinary tract infection. The multidrug resistance exhibited by P. mirabilis renders the treatment ineffective, and new progressive strategies are needed to overcome the antibiotic resistance (AR). We have analyzed the evolutionary relationship of 29 P. mirabilis strains available in the National Center for Biotechnology Information-Genome database. The antimicrobial resistance genes of P. mirabilis along with the enriched pathways and the Gene Ontology terms are analyzed using gene networks to understand the molecular basis of AR. The genes rpoB, tufB, rpsl, fusA, and rpoA could be exploited as potential drug targets as they are involved in regulating the vital functions within the bacterium. The drug targets reported in the present study will aid researchers in developing new strategies to combat multidrug-resistant P. mirabilis.

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Section: Resultsmentioning

confidence: 99%

Gene interaction network approach to elucidate the multidrug resistance mechanisms in the pathogenic bacterial strain Proteus mirabilis

Miryala

Anbarasu

Ramaiah

2020

Journal Cellular Physiology

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“…This enhanced resistance to antimicrobial agents has resulted not only in modifications to antimicrobial therapies, but also in poor diagnosis and increased mortality rate of nosocomial infections [28]. Astonishingly, in 2016, a new isolate of P. mirabilis, from diabetic ulcer patient, have shown a remarkable resistance to silver nanoparticles, spreading the alarm of resistance to even include metallic nanoparticles like silver [29]. Moreover, the efflux pumps (EPs) also play important role in P. mirabilis drug resistance, as exemplified of the increased cell permeability of the EP inhibitor Phenylalanine-Arginine Beta-Naphthylamide (PAβN), thereby making it more susceptible to acetylsalicylic acid [30].…”

Section: Proteus Mirabilismentioning

confidence: 99%

Strategic Role Players of Important Antimicrobial-Resistant Pathogens

Mujawar¹,

Abdella²,

Lahiri³

2021

Antimicrobial Resistance - A One Health Perspective

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Over the years, tireless efforts of the concerned scientists have produced various new therapeutics and methods for the treatment of bacterial infections. However, despite the vast regimen of modern antibiotics being corroborated, the diseases caused by the Gram-positive and-negative pathogens has become untreatable, mainly due to the constantly evolving threat of antimicrobial resistance (AMR), thereby leading to huge morbidity and mortality. Moreover, shortage of efficient therapies, lack of successful prevention strategies and availability of only a few effective antibiotics urgently necessitated the development of novel therapeutics and alternative antimicrobial treatments. These developments have been based on the molecular mechanisms of resistance posed by the pathogens during their interactions with the host. Herein, we collate four essential bacterial components like chaperones, efflux pumps, two-component systems and biofilms which can present challenges for the most coveted control of infection. Essentially, we discuss the current knowledge status of these components to provide insight into the complex regulation of virulence and resistance for some medically important multidrugresistant (MDR) pathogens. This will help the future scientists to clearly focus on some specific proteins to be targeted by against the available class of drugs and/or antibiotics with the broader perspective to develop novel antimicrobial agents.

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“…On the other hand, Proteus spp. genome carries antibiotic resistance loci towards different mechanisms of antimicrobial agents and metals, including swarming mobility, biofilm formation, enzymatic detoxification and efflux systems by employing the PATRIC and PGAAP gene explanation services [75][76][77].…”

Section: Determination Of Antibacterial Activity and Micmentioning

confidence: 99%

Down-Regulation of fliL Gene Expression by Ag Nanoparticles and TiO2 Nanoparticles in Pragmatic Clinical Isolates of Proteus mirabilis and Proteus vulgaris from Urinary Tract Infection

Saleh¹,

Malik²,

Al-Rubaii³

2019

Nano BioMed ENG

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Ten isolates belonging to Proteus spp. were collected and obtained from Department of Biology, College of Science, the University of Baghdad. The diagnosis was done by polymerase chain reaction (PCR) technique using 16S rRNA gene and urease C gene. All isolates (100%) were sensitive to meropenem, imipenem, ciprofloxacin, gentamycin, amoxicillin, clavulanic acid and levofloxacin. These isolates also showed 60% sensitivity to cefixime and nitrofurantoin. However, both species of P. mirabilis and P. vulgaris showed the lowest sensitivity when treated with tetracycline (60%) and amikacin (20%). Cephalothin had a variable effect on the species under study as P. mirabilis isolates were 100% sensitive in comparison with the 80% sensitivity of P. vulgaris isolates. The antibacterial activities of Ag and TiO 2 nanoparticles (NPs) were investigated. The minimum inhibitory concentration (MIC) value of Ag NPs against both species isolates was 10 mg/mL, while the MIC value of TiO 2 NPs was 14 mg/mL against P. mirabilis and 15 mg/mL against P. vulgaris isolates. P. mirabilis isolates showed larger swarming diameter than P. vulgaris, but this motility phenomenon of P. vulgaris was arrested rapidly after incubation with sub-MIC of TiO 2 NPs and Ag NPs comparatively with control. All isolates showed shifting to down-regulation in the fliL gene expression under the effect of the NPs using TiO 2 NPs and Ag NPs. In conclusion, down-regulation of the fliL gene expression is directly linked to the inhibition of swarming movement of Proteus species. We encourage using these inhibitors (after tests to ensure minimal toxicity to human) in combination with antibiotics to ensure bactericidal /bacteriostatic effect to treat Proteus infections.

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Genome sequencing and analysis of the first spontaneous Nanosilver resistant bacteriumProteus mirabilisstrain SCDR1

Abstract: 33Background: P. mirabilis is a common uropathogenic bacterium that can cause major complications in 34

Cited by 4 publications

References 63 publications

Gene interaction network approach to elucidate the multidrug resistance mechanisms in the pathogenic bacterial strain Proteus mirabilis

Gene interaction network approach to elucidate the multidrug resistance mechanisms in the pathogenic bacterial strain Proteus mirabilis

Strategic Role Players of Important Antimicrobial-Resistant Pathogens

Down-Regulation of fliL Gene Expression by Ag Nanoparticles and TiO2 Nanoparticles in Pragmatic Clinical Isolates of Proteus mirabilis and Proteus vulgaris from Urinary Tract Infection

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