Genome Sequences of Three Turkey Orthoreovirus Strains Isolated in Hungary

Dandár, Eszter; Fehér, Enikő; Bálint, Ádám; Kisfali, Péter; Melegh, Béla; Mató, Tamás; Kecskeméti, Sándor; Palya, Vilmos; Bányai, Krisztiàn; Farkas, Szilvia L.

doi:10.1128/genomea.01333-15

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…The genomic organization of all Hungarian study strains was similar and corresponded with that of other reference TRVs, including former Hungarian TRV isolates and TRVs reported from the USA [14,16,[26][27][28]. The full-length genomes were 23,498 bp in seven study strains and 23,494 bp long in a single strain.…”

Section: Genome and Gene-wise Analysessupporting

confidence: 64%

“…When compiling the sequence data for the present study, we realized that genome sequence information for TRV accumulates slowly in public databases. The majority of strains with available genome sequence data comes from the United States, while some genomes were reported from Hungary and a few partial gene sequences are available from Brazil and Croatia [13,16,35]. Even with the advancement of rapid genome amplification and sequencing methods, roughly a dozen TRV whole-genome sequences were available at the time when we started to prepare this paper.…”

Section: Discussionmentioning

confidence: 99%

“…Although TRVs share genomic and phylogenetic similarities with chicken and other galliform bird origin reoviruses, in most instances, they can be distinguished by phylogenetic analyses [13][14][15]. The genetic relationship among TRVs from the Americas and Europe has revealed a very close relationship in past studies [13,16]. In this study, we sequenced eight TRVs isolated from two Hungarian turkey flocks and performed geneby-gene phylogenies in comparison with orthoreoviruses isolated from other galliform birds.…”

Section: Introductionmentioning

confidence: 97%

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A Snapshot on the Genomic Epidemiology of Turkey Reovirus Infections, Hungary

Gál,

Varga-Kugler,

Ihász

et al. 2023

Animals

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Reovirus infections in turkeys are associated with arthritis and lameness. Viral genome sequence data are scarce, which makes an accurate description of the viral evolution and epidemiology difficult. In this study, we isolated and characterized turkey reoviruses from Hungary. The isolates were identified in 2016; these isolates were compared with earlier Hungarian turkey reovirus strains and turkey reoviruses isolated in the 2010s in the United States. Gene-wise sequence and phylogenetic analyses identified the cell-receptor binding protein and the main neutralization antigen, σC, to be the most conserved. The most genetically diverse gene was another surface antigen coding gene, μB. This gene was shown to undergo frequent reassortment among chicken and turkey origin reoviruses. Additional reassortment events were found primarily within members of the homologous turkey reovirus clade. Our data showed evidence for low variability among strains isolated from independent outbreaks, a finding that suggests a common source of turkey reoviruses in Hungarian turkey flocks. Given that commercial vaccines are not available, identification of the source of these founder virus strains would permit a more efficient prevention of disease outbreaks before young birds are settled to fattening facilities.

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Section: Genome and Gene-wise Analysessupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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A Snapshot on the Genomic Epidemiology of Turkey Reovirus Infections, Hungary

Gál,

Varga-Kugler,

Ihász

et al. 2023

Animals

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Whole genome sequence analysis of turkey orthoreovirus isolates reveals a strong viral host-specificity and naturally occurring co-infections in commercial turkeys.

Alvarez Narvaez,

Harrell,

Day

et al. 2024

Virology

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The dynamics of molecular evolution of emerging avian reoviruses through accumulation of point mutations and genetic re-assortment

et al. 2020

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In the last decade, the emergence of variant strains of avian reovirus (ARV) has caused enormous economic impact in the poultry industry across Canada and USA. ARVs are non-enveloped viruses with ten segments of double-stranded RNA genome. So far, only six genotyping cluster groups are identified worldwide based on sequence analysis of the σC protein encoded by the S1 segment. In this study, we performed deep next generation whole-genome sequencing and analysis of twelve purified ARVs isolated from Saskatchewan, Canada. The viruses represent different genotyping cluster. A genome-wide sequence divergence of up to 25 per cent was observed between the virus isolates with a comparable and contrasting evolutionary history. The proportion of synonymous single-nucleotide variations (sSNVs) was higher than the non-synonymous (ns) SNVs across all the genomic segments. Genomic segment S1 was the most variable as compared with the other genes followed by segment M2. Evidence of positive episodic/diversifying selection was observed at different codon positions in the σC protein sequence, which is the genetic marker for the classification of ARV genotypes. In addition, the N-terminus of σC protein had a persuasive diversifying selection, which was not detected in other genomic segments. We identified only four ARV genotypes based on the most variable σC gene sequence. However, a different pattern of phylogenetic clustering was observed with concatenated whole-genome sequences. Together with the accumulation of point mutations, multiple re-assortment events appeared as mechanisms of ARV evolution. For the first time, we determined the mean rate of molecular evolution of ARVs, which was computed as 2.3 × 10−3 substitution/site/year. In addition, widespread geographic intermixing of ARVs was observed between Canada and USA, and between different countries of the world. In conclusion, the study provides a comprehensive analysis of the complete genome of different genotyping clusters of ARVs including their molecular rate of evolution and spatial distribution. The new findings in this study can be utilized for the development of effective vaccines and other control strategies against ARV-induced arthritis/tenosynovitis in the poultry industry worldwide.

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Genome Sequences of Three Turkey Orthoreovirus Strains Isolated in Hungary

Cited by 4 publications

References 13 publications

A Snapshot on the Genomic Epidemiology of Turkey Reovirus Infections, Hungary

A Snapshot on the Genomic Epidemiology of Turkey Reovirus Infections, Hungary

Whole genome sequence analysis of turkey orthoreovirus isolates reveals a strong viral host-specificity and naturally occurring co-infections in commercial turkeys.

The dynamics of molecular evolution of emerging avian reoviruses through accumulation of point mutations and genetic re-assortment

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