Genome Sequences of Three Strains of Lactobacillus paracasei of Different Origins and with Different Cholate Sensitivities

Abstract

We report here the draft genome sequences of three strains of Lactobacillus paracasei (NRIC 0644, NRIC 1781, and NRIC 1917) isolated from different sources. The three genomes range from 2.95 to 3.15 Mb with a G+C content of 46% and contain approximately 2,700 protein coding sequences.

Identification of sirtuin and its target as the ribosomal protein S4 in <i>Lactobacillus paracasei</i>

“…The details of the methodology and the basic data of the genome analysis of the strains used in this report have been published elsewhere (Shiwa et al, 2015). In this study, identification of sirtuin genes in the draft genomes was performed by using the data analysis program, In Silico Molecular Cloning (In Silico Biotechnology, Yokohama, Japan).…”

“…Lactobacillus paracasei strains NRIC 0644, NRIC 1917 and NRIC 1981 were obtained from the NRIC culture collection (Tokyo University of Agriculture, Japan). Strains NRIC 1917 andNRIC 1981 were isolated from compost and sugar cane wine, respectively, whereas the origin of NRIC 0644 was unknown (Shiwa et al, 2015). Polyclonal rabbit anti-sera were raised against purified recombinant LpSirA (this study), and were affinity-purified by Japan Lamb Ltd. (Hiroshima, Japan).…”

“…In the latter case, LAB can function in a more beneficial manner when they stay alive in the gastrointestinal canals. In a previous study, we determined draft genome sequences of three L. paracasei strains isolated from either an animal or a plant origin with a different cholate resistance (Shiwa et al, 2015). In this study, sirtuin homolog genes of these strains, designated sirA and sirB, have been cloned, and corresponding recombinant proteins have been generated and purified for enzyme kinetic characterization.…”

Detection and analysis of <i>Lactobacillus paracasei </i>penicillin-binding proteins revealed the presence of cholate-sensitive penicillin-binding protein 3 and an elongated cell shape in a cholate-sensitive strain

“…Functional information is not available for these LpPBPs. Moreover, the draft genome data of the genes annotated as putative penicillin-binding protein homologs in L. paracasei strains used in this study [ 11 ] exhibited very low homology (with calculated sizes ranging between 75−83 kDa) when compared with those of genes for E. coli PBPs (data not shown). Instead, the LpPBPs demonstrated in this study gave a tangible image of “penicillin-binding” proteins in L. paracasei (with apparent molecular weights ranging between 39−96 kDa), appeared after SDS gel electrophoresis and subsequent localization by immunoblotting ( Fig.…”