Genome Sequences of Three
            <i>Lactobacillus</i>
            Species Strains of the Stomach of the White-Footed Deermouse (Peromyscus leucopus)

Bassam, Khalil; Milovic, Ana; Barbour, Alan G.

doi:10.1128/mra.00963-19

Cited by 2 publications

(5 citation statements)

References 7 publications

(7 reference statements)

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“…LL6 and provisionally named as “L. peromysci”, have been reported [ 24 ]. The fourth genome, of the LL8 strain of L .…”

Section: Resultsmentioning

confidence: 99%

“…Three organisms represented among the high coverage contigs could be unambiguously classified as to species: Lactobacillus animalis, which is considered in detail below, and two Parabacteroides species: distasonis and johnsonii. Another Lactobacillus species represented among the highly ranked contigs could not be identified with a known species represented in the database [24].…”

Section: High Coverage Sequencing Of Fecal Metagenomementioning

confidence: 97%

“…The genomes of three of organisms, namely L. animalis strain LL1, L. reuteri strain LL7, and a new species, designated as Lactobacillus sp. LL6 and provisionally named as "L. peromysci", have been reported [24]. The fourth genome, of the LL8 strain of L. johnsonii, is described first here (Table 1).…”

Section: Plos Onementioning

confidence: 99%

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Lactobacilli and other gastrointestinal microbiota of Peromyscus leucopus, reservoir host for agents of Lyme disease and other zoonoses in North America

et al. 2020

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The cricetine rodent Peromyscus leucopus is an important reservoir for several human zoonoses, including Lyme disease, in North America. Akin to hamsters, the white-footed deermouse has been unevenly characterized in comparison to the murid Mus musculus. To further understanding of P. leucopus' total genomic content, we investigated gut microbiomes of an outbred colony of P. leucopus, inbred M. musculus, and a natural population of P. leucopus. Metagenome and whole genome sequencing were combined with microbiology and microscopy approaches. A focus was the genus Lactobacillus, four diverse species of which were isolated from forestomach and feces of colony P. leucopus. Three of the species-L. animalis, L. reuteri, and provisionally-named species "L. peromysci"-were identified in fecal metagenomes of wild P. leucopus but not discernibly in samples from M. musculus. L. johnsonii, the fourth species, was common in M. musculus but absent or sparse in wild P. leucopus. Also identified in both colony and natural populations were a Helicobacter sp. in feces but not stomach, and a Tritrichomonas sp. protozoan in cecum or feces. The gut metagenomes of colony P. leucopus were similar to those of colony M. musculus at the family or higher level and for major subsystems. But there were multiple differences between species and sexes within each species in their gut metagenomes at orthologous gene level. These findings provide a foundation for hypothesis-testing of functions of individual microbial species and for interventions, such as bait vaccines based on an autochthonous bacterium and targeting P. leucopus for transmission-blocking.

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“…LL6 and provisionally named as “L. peromysci”, have been reported [ 24 ]. The fourth genome, of the LL8 strain of L .…”

Section: Resultsmentioning

confidence: 99%

Section: High Coverage Sequencing Of Fecal Metagenomementioning

confidence: 97%

See 1 more Smart Citation

Lactobacilli and other gastrointestinal microbiota of Peromyscus leucopus, reservoir host for agents of Lyme disease and other zoonoses in North America

et al. 2020

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show abstract

“…LL6 and provisionally named as “L. peromysci”, have been reported (24). The fourth genome, of the LL8 strain of L. johnsonii , is described first here (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…The accession numbers for the annotated genomes and plasmids of L. animalis LL1, L. reuteri LL7, and “L. peromysci” LL6 are given in Bassam et al (24). Fig 2 and its legend provides accession numbers for 16S ribosomal RNA gene sequences of other Lactobacillus species and strains.…”

Section: Methodsmentioning

confidence: 99%

Lactobacilli and other gastrointestinal microbiota of Peromyscus leucopus, reservoir host for agents of Lyme disease and other zoonoses in North America

Barbour

Milovic

Bassam

et al. 2020

Preprint

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The cricetine rodent Peromyscus leucopus is an important reservoir for several human zoonoses, including Lyme disease, in North America. Akin to hamsters, the white-footed deermouse has been unevenly characterized in comparison to the murid Mus musculus. To further understanding of P. leucopus’ total genomic content, we investigated gut microbiomes of an outbred colony of P. leucopus, inbred M. musculus, and a natural population of P. leucopus. Metagenome and whole genome sequencing were combined with microbiology and microscopy approaches. A focus was the genus Lactobacillus, four diverse species of which were isolated from forestomach and feces of colony P. leucopus. Three of the species--L. animalis, L. reuteri, and provisionally-named species “L. peromysci”--were identified in fecal metagenomes of wild P. leucopus but not discernibly in samples from M. musculus. L. johnsonii, the fourth species, was common in M. musculus but absent or sparse in wild P. leucopus. Also identified in both colony and natural populations were a Helicobacter sp. in feces but not stomach, and a Tritrichomonas sp. protozoan in cecum or feces. The gut metagenomes of colony P. leucopus were similar to those of colony M. musculus at the family or higher level and for major subsystems. But there were multiple differences between species and sexes within each species in their gut metagenomes at orthologous gene level. These findings provide a foundation for hypothesis-testing of functions of individual microbial species and for interventions, such as bait vaccines based on an autochthonous bacterium and targeting P. leucopus for transmission-blocking.

show abstract

Genome Sequences of Three Lactobacillus Species Strains of the Stomach of the White-Footed Deermouse (Peromyscus leucopus)

Cited by 2 publications

References 7 publications

Lactobacilli and other gastrointestinal microbiota of Peromyscus leucopus, reservoir host for agents of Lyme disease and other zoonoses in North America

Lactobacilli and other gastrointestinal microbiota of Peromyscus leucopus, reservoir host for agents of Lyme disease and other zoonoses in North America

Lactobacilli and other gastrointestinal microbiota of Peromyscus leucopus, reservoir host for agents of Lyme disease and other zoonoses in North America

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