Genome Sequences of Soft Rot-Causing Pseudomonas Isolates from Spinach

Wasendorf, Chloe; Schultz, Dylan L.; Schmitz‐Esser, Stephan; Peters, Nick T.

doi:10.1128/mra.00701-22

Cited by 2 publications

(3 citation statements)

References 14 publications

(9 reference statements)

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“…Of these, strain ICMP 14937, isolated from the head rot of broccoli in Iran, has been identified as the pathogen of the disease. In addition, Wasendorf et al (2022) isolated the pathogens from soft rot‐diseased spinach leaves in the United States, of which they found that strain SR18 exhibited an ANI (average nucleotide identity) value of 96.1% against the type strain of P . cyclaminis , suggesting that SR18 may belong to P .…”

Section: Soft Rot‐causing Species Derived From P Marginalis Sensu Latomentioning

confidence: 99%

“…petroselini , we obtained high values of 98.94% and 90.9%, respectively (authors' unpublished data). Furthermore, Wasendorf et al (2022) reported that strain SR17 isolated from soft rot‐diseased spinach leaves in the United States exhibited an ANI value of 98.9% against ICMP 3553. These results indicate that both ICMP 3553 and SR17 belong to P .…”

Section: Soft Rot‐causing Species Derived From P Marginalis Sensu Latomentioning

confidence: 99%

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Dismantling and reorganizing Pseudomonas marginalis sensu lato

2022

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Until recently, it had been a common practice in the field of plant disease control to identify bacterial strains with the following characteristics as Pseudomonas marginalis: fluorescent; oxidase‐, potato soft rot‐ and arginine dihydrolase‐positive; soft rot‐causing (here referred to as P. marginalis sensu lato). In contrast, as phylogenetic and genomic analyses have been introduced into the bacterial taxonomy, it became clear that P. marginalis sensu lato is heterogeneous, suggesting that it might be a species complex containing many cryptic species. Therefore, a preliminary survey was performed targeting the P. marginalis sensu lato strains preserved in the NARO (National Agriculture and Food Research Organization, Japan) Genebank to sort these into strains corresponding to P. marginalis sensu stricto (i.e., strains that would be included in the same species range as the type strain of P. marginalis) and strains that might not fit this category. Since then, attempts have been made to examine the taxonomic affiliations of the latter by using a polyphasic approach. As a result, some strains were proven to differ from any known species and are described as the following novel species: Pseudomonas kitaguniensis, Pseudomonas allii, Pseudomonas cyclaminis, Pseudomonas petroselini, Pseudomonas brassicae, Pseudomonas lactucae, Pseudomonas aegrilactucae and Pseudomonas morbosilactucae. In this article, the profiles of each of these novel species and a scheme for their identification are briefly introduced. In addition to the problem of cryptic species, reorganizing P. marginalis sensu lato still faces various challenges, which we discuss.

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Section: Soft Rot‐causing Species Derived From P Marginalis Sensu Latomentioning

confidence: 99%

Section: Soft Rot‐causing Species Derived From P Marginalis Sensu Latomentioning

confidence: 99%

Dismantling and reorganizing Pseudomonas marginalis sensu lato

2022

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“…The genera Dickeya and Pectobacterium, belonging to the family Pectobacteriaceae, are the most extensively studied bacterial soft rot pathogens and have caused constant problems in vegetable production, particularly in the case of potatoes (Charkowski, 2018;Park et al, 2023). In addition to the Dickeya and Pectobacterium species, mesophilic and psychrotolerant (cold-adapted) Pseudomonas strains of the family Pseudomonadaceae have recently emerged as significant pathogens affecting carrots, onions, lettuce, melons, spinach, and potatoes (Charkowski, 2018;Godfrey and Marshall, 2002;González et al, 2003;Kim et al, 2002;Wasendorf et al, 2022;Zhang et al, 2016)…”

Section: Introductionmentioning

confidence: 99%

Potato Soft Rot Caused by Psychrotolerant Pseudomonas sp. from Subarctic Tundra Soil

Woo,

Lee,

Kim

2023

Res. Plant Dis

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Agricultural activities and the number of farms in the subarctic regions have been increasing annually after the coronavirus disease 2019 pandemic to achieve food self-sufficiency. Potatoes are vulnerable to soft rot bacteria at all stages of production, storage, and transportation. A novel bacterium, <i>Pseudomonas</i> sp. N3-W, isolated from Alaska tundra soil, grows at 5–25°C and produces extracellular protease(s). N3-W caused necrotic spots (hypersensitivity) in hot pepper leaves and soft rot disease (pathogenicity) in potato tubers. The psychrotolerant N3-W caused significant soft rot symptoms on potatoes at a broad temperature range (5°C, 15°C, and 25°C). In contrast, mesophilic <i>Pectobacterium carotovorum</i> KACC 16999 induced severe rotting symptoms in potatoes at their optimal growth temperature of 15°C and 25°C. However, it barely produced symptoms at 5°C, which is the appropriate storage and transportation temperature for potatoes. The results of pathogenicity testing imply that psychrotolerant soft rot pathogens from polar regions may cause severe soft rot not only during the crop growing season but also during storage and transportation. Our study indicates the possibility of new plant pathogen emergence and transmission due to the expansion of crop cultivation areas caused by permafrost thawing in response to recent polar warming.

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Genome Sequences of Soft Rot-Causing Pseudomonas Isolates from Spinach

Abstract: Two Pseudomonas strains (SR17 and SR18) were isolated from soft rot-diseased spinach leaves. Here, we report their genome sequences and characteristics.

Cited by 2 publications

References 14 publications

Dismantling and reorganizing Pseudomonas marginalis sensu lato

Dismantling and reorganizing Pseudomonas marginalis sensu lato

Potato Soft Rot Caused by Psychrotolerant Pseudomonas sp. from Subarctic Tundra Soil

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