Genome Sequences of Newly Isolated Mycobacteriophages Forming Cluster S

Abstract

We describe the genomes of two mycobacteriophages, MosMoris and Gattaca, newly isolated on Mycobacterium smegmatis. The two phages are very similar to each other, differing in 61 single nucleotide polymorphisms and six small insertion/deletions. Both have extensive nucleotide sequence similarity to mycobacteriophage Marvin and together form cluster S.

<p>Directed evolution as a tool for the selection of oncolytic RNA viruses with desired phenotypes</p>

“…Mutation accumulation was associated with a significant decrease of SeV oncolytic activity toward melanoma (Mel8) and glioma (U87MG) cells 77. Reverse passaging in embryonated chicken eggs of SeV, which had been adapted to grow in the 4,647-cell line, partially restored its oncolytic activity 77. The reverse changes in the virus phenotype corresponded to reverse changes in its genotype.…”

“…SeV propagation in cell culture might be a cheaper alternative to virus growth in eggs. In a study by Zainutdinov and colleagues77 two cell lines were chosen for culture of SeV variants, 4,647 (African green monkey kidney cells) and HEK293 (human embryonic kidney cells). Both cell lines are certified for viral vaccine production in Russia.…”

“…Figure 3 shows the scheme of adaptation experiment that included multiple (20–25 times) passaging of SeV in both cell cultures. The passaging caused numerous non-synonymous nucleotide substitutions 77. Most of these substitutions were in the F and HN genes, which encode surface proteins of the SeV virion 77.…”

“…The passaging caused numerous non-synonymous nucleotide substitutions 77. Most of these substitutions were in the F and HN genes, which encode surface proteins of the SeV virion 77. Mutation accumulation was associated with a significant decrease of SeV oncolytic activity toward melanoma (Mel8) and glioma (U87MG) cells 77.…”

“…Most of these substitutions were in the F and HN genes, which encode surface proteins of the SeV virion 77. Mutation accumulation was associated with a significant decrease of SeV oncolytic activity toward melanoma (Mel8) and glioma (U87MG) cells 77. Reverse passaging in embryonated chicken eggs of SeV, which had been adapted to grow in the 4,647-cell line, partially restored its oncolytic activity 77.…”