Genome Sequences of
            <i>Gordonia terrae</i>
            Bacteriophages Phinally and Vivi2

Pope, Welkin H.; Anderson, Kaitlyn C.; Arora, Charu; Bortz, Michael E.; Burnet, G.; Conover, David H.; D'Incau, Gina M.; Ghobrial, Jonathan A.; Jonas, Audrey L.; Migdal, Emily J.; Rote, Nicole L.; German, Brian A.; McDonnell, Jill E.; Mezghani, Nadia; Schafer, Claire E.; Thompson, Paige K.; Ulbrich, Megan C.; Yu, Victor J.; Furbee, Emily C.; Grubb, Sarah R.; Warner, Marcie H.; Montgomery, Matthew T.; Garlena, Rebecca A.; Russell, Daniel A.; Jacobs-Sera, Deborah; Hatfull, Graham F.

doi:10.1128/genomea.00599-16

Cited by 1 publication

(2 citation statements)

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“…Sixty phages isolated on Gordonia spp. through enrichment culture or direct plating as part of the Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) and the Phage Hunters Integrating Research and Education (PHIRE) programs ( 15 , 18 – 28 ) were used for comparative analysis ( Table 1 ), together with 19 Gordonia phages described previously ( 13 , 16 , 17 ). All but two of the SEA-PHAGES/PHIRE phages were isolated on G. terrae 3612; the other two were isolated on Gordonia neofelifaecis ( 34 ); the 19 previously described phages were isolated on Gordonia spp., including G. terrae , G. rubripertincta , G. malaquae , G. sputi , or G. alkanivorans ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

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Bacteriophages of Gordonia spp. Display a Spectrum of Diversity and Genetic Relationships

Pope

Mavrich

Garlena

et al. 2017

mBio

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The global bacteriophage population is large, dynamic, old, and highly diverse genetically. Many phages are tailed and contain double-stranded DNA, but these remain poorly characterized genomically. A collection of over 1,000 phages infecting Mycobacterium smegmatis reveals the diversity of phages of a common bacterial host, but their relationships to phages of phylogenetically proximal hosts are not known. Comparative sequence analysis of 79 phages isolated on Gordonia shows these also to be diverse and that the phages can be grouped into 14 clusters of related genomes, with an additional 14 phages that are “singletons” with no closely related genomes. One group of six phages is closely related to Cluster A mycobacteriophages, but the other Gordonia phages are distant relatives and share only 10% of their genes with the mycobacteriophages. The Gordonia phage genomes vary in genome length (17.1 to 103.4 kb), percentage of GC content (47 to 68.8%), and genome architecture and contain a variety of features not seen in other phage genomes. Like the mycobacteriophages, the highly mosaic Gordonia phages demonstrate a spectrum of genetic relationships. We show this is a general property of bacteriophages and suggest that any barriers to genetic exchange are soft and readily violable.

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Section: Resultsmentioning

confidence: 99%

“…Sixty phages were isolated on Gordonia spp. through enrichment culture or direct plating as part of the SEA-PHAGES and PHIRE programs, and dsDNA was extracted ( 15 , 18 – 28 ). Double-stranded DNA genomes were sequenced using Illumina Mi-Seq and assembled using Newbler and Consed.…”

Section: Methodsmentioning

confidence: 99%