Genome Sequences of 11 Conspecific
            <i>Streptomyces</i>
            sp. Strains

Tidjani, Abdoul-Razak; Lorenzi, Jean-Noël; Toussaint, Maxime; Dijk, Erwin van; Naquin, Delphine; Lespinet, Olivier; Bontemps, Cyril; Leblond, Pierre

doi:10.1128/mra.00863-19

Cited by 4 publications

(9 citation statements)

References 9 publications

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“…Terminal inverted repeats (TIRs) identification. Genome sequencing of the Streptomyces chromosomes was previously reported 24 . It consists in one scaffold per chromosome and per extra-chromosomal element when present.…”

Section: Methodsmentioning

confidence: 99%

“…After identification of the TIRs, their extremities were extended using the Illumina sequencing read batch using CLC Genomics Workbench (v6.0, Qiagen) in order to find or complete the telomere sequence; for nine of the 11 strains as well as for the three linear plasmids (pRLB1-9.2, pS1D4-14.1, pS1D4-20.1), this procedure allowed to extend the sequence and identify telomere sequences. Updated versions of the chromosomes and plasmid sequence are accessible under the previous accession numbers (24).…”

Section: Methodsmentioning

confidence: 99%

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Telomeric and sub-telomeric regions undergo rapid turnover within a Streptomyces population

Tidjani

Bontemps

Leblond

2020

Sci Rep

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Genome dynamics was investigated within natural populations of the soil bacterium Streptomyces. The exploration of a set of closely related strains isolated from micro-habitats of a forest soil exhibited a strong diversity of the terminal structures of the linear chromosome, i.e. terminal inverted repeats (TIRs). Large insertions, deletions and translocations could be observed along with evidence of transfer events between strains. In addition, the telomere and its cognate terminal protein complexes required for terminal replication and chromosome maintenance, were shown to be variable within the population probably reflecting telomere exchanges between the chromosome and other linear replicons (i.e., plasmids). Considering the close genetic relatedness of the strains, these data suggest that the terminal regions are prone to a high turnover due to a high recombination associated with extensive horizontal gene transfer.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Telomeric and sub-telomeric regions undergo rapid turnover within a Streptomyces population

Tidjani

Bontemps

Leblond

2020

Sci Rep

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“…All the isolates belonged to the Streptomyces genus and eight strains were retained for further analyses. The three strains (RLB1-8, RLB1-9, and S1D4-23) were already described and their genome sequenced in Tidjani et al [32]. These strains were highly related and were part of the same population.…”

Section: Isolation and Taxonomic Characterization Of A Streptomyces Communitymentioning

confidence: 99%

Mining the Biosynthetic Potential for Specialized Metabolism of a Streptomyces Soil Community

et al. 2020

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The diversity and distribution of specialized metabolite gene clusters within a community of bacteria living in the same soil habitat are poorly documented. Here we analyzed the genomes of 8 Streptomyces isolated at micro-scale from a forest soil that belong to the same species or to different species. The results reveal high levels of diversity, with a total of 261 biosynthesis gene clusters (BGCs) encoding metabolites such as terpenes, polyketides (PKs), non-ribosomal peptides (NRPs) and ribosomally synthesized and post-translationally modified peptides (RiPPs) with potential bioactivities. A significant part of these BGCs (n = 53) were unique to only one strain when only 5 were common to all strains. The metabolites belong to very diverse chemical families and revealed that a large diversity of metabolites can potentially be produced in the community. Although that analysis of the global metabolome using GC-MS revealed that most of the metabolites were shared between the strains, they exhibited a specific metabolic pattern. We also observed that the presence of these accessory pathways might result from frequent loss and gain of genes (horizontal transfer), showing that the potential of metabolite production is a dynamic phenomenon in the community. Sampling Streptomyces at the community level constitutes a good frame to discover new biosynthetic pathways and it appears as a promising reservoir for the discovery of new bioactive compounds.

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“…to a previously isolated cluster of strains (identical 16S rRNA gene sequences) (8,14) that was closely related to Streptomyces olivochromogenes DSM 40451 (99.9% identity in 16S rRNA gene sequences) (15). The strains RLB1-33, RPA4-2, and S1D4-11 and Streptomyces mirabilis CSSP107 exhibited identities of 99.9% to 99.2% in their 16S rRNA genes, indicating that they might belong to the same species or constitute sister species.…”

mentioning

confidence: 81%

Genome Sequences of Five Streptomyces Strains Isolated at Microscale

Nicault

Tidjani

Gelhaye

et al. 2020

Microbiol Resour Announc

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Genome Sequences of 11 Conspecific Streptomyces sp. Strains

Abstract: The genomes of 11 conspecific Streptomyces strains, i.e., from the same species and inhabiting the same ecological niche, were sequenced and assembled. This data set offers an ideal framework to assess the genome evolution of Streptomyces species in their ecological context.

Cited by 4 publications

References 9 publications

Telomeric and sub-telomeric regions undergo rapid turnover within a Streptomyces population

Telomeric and sub-telomeric regions undergo rapid turnover within a Streptomyces population

Mining the Biosynthetic Potential for Specialized Metabolism of a Streptomyces Soil Community

Genome Sequences of Five Streptomyces Strains Isolated at Microscale

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