Genome Sequence of the Tobacco Bacterial Wilt Pathogen Ralstonia solanacearum

Li, Zefeng; Wu, Sanling; Bai, Xin; Liu, Yun; Lu, Jian-Fei; Liu, Yong; Xiao, Bingguang; Lu, Xiaoxuan; Fan, Longjiang

doi:10.1128/jb.06009-11

Cited by 56 publications

(35 citation statements)

References 17 publications

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“…We could not analyze strains Po82, FQY-4, and Y45 since the authors of these published genomes would not share their strains (Li et al, 2011; Xu et al, 2011; Cao et al, 2013). Each strain was tested for its ability to grow on the HCAs ferulate (Fer) and p -coumarate (Cou) as well as on the pathway intermediates vanillin (Van), vanillate (VA), p -hydroxybenzoate (HBA), and protocatechuate (PCA) (see Fig.…”

Section: Resultsmentioning

confidence: 99%

Hydroxycinnamic Acid Degradation, a Broadly Conserved Trait, ProtectsRalstonia solanacearumfrom Chemical Plant Defenses and Contributes to Root Colonization and Virulence

Lowe¹,

Ailloud²,

Allen³

2015

MPMI

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Plants produce hydroxycinnamic acid defense compounds (HCAs) to combat pathogens, such as the bacterium Ralstonia solanacearum. We showed that an HCA degradation pathway is genetically and functionally conserved across diverse R. solanacearum strains. Further, a Δfcs (feruloyl-CoA synthetase) mutant that cannot degrade HCAs was less virulent on tomato plants. To understand the role of HCA degradation in bacterial wilt disease, we tested the following hypotheses: HCA degradation helps the pathogen (1) grow, as a carbon source; (2) spread, by reducing physical barriers HCA-derived; and (3) survive plant antimicrobial compounds. Although HCA degradation enabled R. solanacearum growth on HCAs in vitro, HCA degradation was dispensable for growth in xylem sap and root exudate, suggesting that HCAs are not significant carbon sources in planta. Acetyl-bromide quantification of lignin demonstrated that R. solanacearum infections did not affect the gross quantity or distribution of stem lignin. However, the Δfcs mutant was significantly more susceptible to inhibition by two HCAs: caffeate and p-coumarate. Finally, plant colonization assays suggested that HCA degradation facilitates early stages of infection and root colonization. Together, these results indicated that ability to degrade HCAs contributes to bacterial wilt virulence by facilitating root entry and by protecting the pathogen from HCA toxicity.

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Section: Resultsmentioning

confidence: 99%

Hydroxycinnamic Acid Degradation, a Broadly Conserved Trait, ProtectsRalstonia solanacearumfrom Chemical Plant Defenses and Contributes to Root Colonization and Virulence

Lowe¹,

Ailloud²,

Allen³

2015

MPMI

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“…Based on a combination of genotypic and phenotypic data of a large set of strains of the R. solanacearum species complex obtained in this study and on additional analyses of genome sequences, comparative genome hybridization arrays and MLSA data (Cao et al, 2013;Gabriel et al, 2006;Guidot et al, 2009;Li et al, 2011;Remenant et al, 2010Remenant et al, , 2011Salanoubat et al, 2002;Wicker et al, 2012;Xu et al, 2011), the following taxonomic proposals are made. The species Ralstonia solanacearum is limited to strains of (14) 56 (9) 54 (1) 53 (1) 75 (8) 73 (26) Table 4.…”

Section: Ralstonia Syzygii Subsp Celebesensis Uqrs 627 T (Kc757025)mentioning

confidence: 99%

Polyphasic taxonomic revision of the Ralstonia solanacearum species complex: proposal to emend the descriptions of Ralstonia solanacearum and Ralstonia syzygii and reclassify current R. syzygii strains as Ralstonia syzygii subsp. syzygii subsp. nov., R. solanacearum phylotype IV strains as Ralstonia syzygii subsp. indonesiensis subsp. nov., banana blood disease bacterium strains as Ralstonia syzygii subsp. celebesensis subsp. nov. and R. solanacearum phylotype I and III strains as Ralstonia pse

Safni

Cleenwerck

Vos

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

388

261

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The Ralstonia solanacearum species complex has long been recognized as a group of phenotypically diverse strains that can be subdivided into four phylotypes. Using a polyphasic taxonomic approach on an extensive set of strains, this study provides evidence for a taxonomic and nomenclatural revision of members of this complex. Data obtained from phylogenetic analysis of 16S-23S rRNA ITS gene sequences, 16S–23S rRNA intergenic spacer (ITS) region sequences and partial endoglucanase (egl) gene sequences and DNA–DNA hybridizations demonstrate that the R. solanacearum species complex comprises three genospecies. One of these includes the type strain of Ralstonia solanacearum and consists of strains of R. solanacearum phylotype II only. The second genospecies includes the type strain of Ralstonia syzygii and contains only phylotype IV strains. This genospecies is subdivided into three distinct groups, namely R. syzygii , the causal agent of Sumatra disease on clove trees in Indonesia, R. solanacearum phylotype IV strains isolated from different host plants mostly from Indonesia, and strains of the blood disease bacterium (BDB), the causal agent of the banana blood disease, a bacterial wilt disease in Indonesia that affects bananas and plantains. The last genospecies is composed of R. solanacearum strains that belong to phylotypes I and III. As these genospecies are also supported by phenotypic data that allow the differentiation of the three genospecies, the following taxonomic proposals are made: emendation of the descriptions of Ralstonia solanacearum and Ralstonia syzygii and descriptions of Ralstonia syzygii subsp. nov. (type strain R 001T = LMG 10661T = DSM 7385T) for the current R. syzygii strains, Ralstonia syzygii subsp. indonesiensis subsp. nov. (type strain UQRS 464T = LMG 27703T = DSM 27478T) for the current R. solanacearum phylotype IV strains, Ralstonia syzygii subsp. celebesensis subsp. nov. (type strain UQRS 627T = LMG 27706T = DSM 27477T) for the BDB strains and Ralstonia pseudosolanacearum sp. nov. (type strain UQRS 461T = LMG 9673T = NCPPB 1029T) for the strains of R. solanacearum phylotypes I and III.

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“…Three fully sequenced and annotated genomes (phylotype I strains GMI1000 [46] and Y45 [49] and phylotype III strain CMR15 [43]) that are publicly available via the MaGe interface (RalstoniaScope [76]) were screened for detection of tandem repeats using the Microorganisms Tandem Repeats Database (MTRDB) (77,78) and the Tandem Repeats Database (TRDB) (79). It has been reported that the number of alleles correlates with the number of TR in the genome and conversely correlates with the pattern size (80), but so far, no general rules have been published.…”

Section: Methodsmentioning

confidence: 99%

“…This phylotype is thus an interesting model for studying the evolutionary dynamics of plant-bacterium interactions. Although the molecular basis of its interactions with plants and the variability of its genome have been investigated widely (43)(44)(45)(46)(47)(48)(49), the population genetics and dynamics of this fascinating plant pathogen remain poorly understood and considered. Until now, only two studies have addressed the population genetic structure on a local scale.…”

Section: Importancementioning

confidence: 99%

New Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) Scheme for Fine-Scale Monitoring and Microevolution-Related Study of Ralstonia pseudosolanacearum Phylotype I Populations

Guinard

Latreille

Guérin

et al. 2017

Appl Environ Microbiol

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Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) is considered one of the most harmful plant diseases in the world. Special attention should be paid to R. pseudosolanacearum phylotype I due to its large host range, its worldwide distribution, and its high evolutionary potential. So far, the molecular epidemiology and population genetics of this bacterium are poorly understood. Until now, the genetic structure of the RSSC has been analyzed on the worldwide and regional scales. Emerging questions regarding evolutionary forces in RSSC adaptation to hosts now require genetic markers that are able to monitor RSSC field populations. In this study, we aimed to evaluate the multilocus variable-number tandem-repeat analysis (MLVA) approach for its ability to discriminate genetically close phylotype I strains and for population genetics studies. We developed a new MLVA scheme (MLVA-7) allowing us to genotype 580 R. pseudosolanacearum phylotype I strains extracted from susceptible and resistant hosts and from different habitats (stem, soil, and rhizosphere). Based on specificity, polymorphism, and the amplification success rate, we selected seven fast-evolving variable-number tandem-repeat (VNTR) markers. The newly developed MLVA-7 scheme showed higher discriminatory power than the previously published MLVA-13 scheme when applied to collections sampled from the same location on different dates and to collections from different locations on very small scales. Our study provides a valuable tool for fine-scale monitoring and microevolution-related study of R. pseudosolanacearum phylotype I populations.IMPORTANCE Understanding the evolutionary dynamics of adaptation of plant pathogens to new hosts or ecological niches has become a key point for the development of innovative disease management strategies, including durable resistance. Whereas the molecular mechanisms underlying virulence or pathogenicity changes have been studied thoroughly, the population genetics of plant pathogen adaptation remains an open, unexplored field, especially for plant-pathogenic bacteria. MLVA has become increasingly popular for epidemiosurveillance and molecular epidemiology studies of plant pathogens. However, this method has been used mostly for genotyping and identification on a regional or global scale. In this study, we developed a new MLVA scheme, targeting phylotype I of the soilborne Ralstonia solanacearum species complex (RSSC), specifically to address the bacterial population genetics on the field scale. Such a MLVA scheme, based on fast-

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Genome Sequence of the Tobacco Bacterial Wilt Pathogen Ralstonia solanacearum

Cited by 56 publications

References 17 publications

Hydroxycinnamic Acid Degradation, a Broadly Conserved Trait, ProtectsRalstonia solanacearumfrom Chemical Plant Defenses and Contributes to Root Colonization and Virulence

Hydroxycinnamic Acid Degradation, a Broadly Conserved Trait, ProtectsRalstonia solanacearumfrom Chemical Plant Defenses and Contributes to Root Colonization and Virulence

New Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) Scheme for Fine-Scale Monitoring and Microevolution-Related Study of Ralstonia pseudosolanacearum Phylotype I Populations

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