Genome Sequence of the Nonconventional Wine Yeast
            <i>Hanseniaspora guilliermondii</i>
            UTAD222

Seixas, Isabel; Barbosa, Catarina; Salazar, Sara B.; Mendes‐Faia, Arlete; Wang, Yu; Güldener, Ulrich; Mendes‐Ferreira, Ana; Mira, Nuno P.

doi:10.1128/genomea.01515-16

Cited by 9 publications

(11 citation statements)

References 9 publications

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“…Karyotyping approaches suggested the presence of 7 to 9 chromosomes, with high variability between different isolates (Esteve-Zarzoso et al, 2001;Cadez et al, 2002), whereas the mitochondrial genome of H. uvarum has an exceptional structure among fungi, as it is represented by a short, linear DNA molecule (Pramateftaki et al, 2006). Recently, some brief reports on wholegenome approaches have appeared for Hanseniaspora strains, underlining the growing interest in this yeast, but with limited information regarding genome annotations (Giorello et al, 2014;Sternes et al, 2016;Seixas et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Genome Sequencing and Comparative Analysis of Three Hanseniaspora uvarum Indigenous Wine Strains Reveal Remarkable Biotechnological Potential

et al. 2020

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A current trend in winemaking has highlighted the beneficial contribution of non-Saccharomyces yeasts to wine quality. Hanseniaspora uvarum is one of the more represented non-Saccharomyces species onto grape berries and plays a critical role in influencing the wine sensory profile, in terms of complexity and organoleptic richness. In this work, we analyzed a group of H. uvarum indigenous wine strains as for genetic as for technological traits, such as resistance to SO 2 and β-glucosidase activity. Three strains were selected for genome sequencing, assembly and comparative genomic analyses at species and genus level. Hanseniaspora genomes appeared compact and contained a moderate number of genes, while rarefaction analyses suggested an open accessory genome, reflecting a rather incomplete representation of the Hanseniaspora gene pool in the currently available genomes. The analyses of patterns of functional annotation in the three indigenous H. uvarum strains showed distinct enrichment for several PFAM protein domains. In particular, for certain traits, such as flocculation related protein domains, the genetic prediction correlated well with relative flocculation phenotypes at lab-scale. This feature, together with the enrichment for oligo-peptide transport and lipid and amino acid metabolism domains, reveals a promising potential of these indigenous strains to be applied in fermentation processes and modulation of wine flavor and aroma. This study also contributes to increasing the catalog of publicly available genomes from H. uvarum strains isolated from natural grape samples and provides a good roadmap for unraveling the biodiversity and the biotechnological potential of these non-Saccharomyces yeasts.

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Section: Introductionmentioning

confidence: 99%

Genome Sequencing and Comparative Analysis of Three Hanseniaspora uvarum Indigenous Wine Strains Reveal Remarkable Biotechnological Potential

et al. 2020

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“…While the environment occupied by the Hanseniaspora last common ancestor is unknown, it is plausible that environmental instability or other stressors favored hypermutators in Hanseniaspora . Extant Hanseniaspora species are well known to be associated with the grape environment [40,85,86]. Interestingly, grapes appear to have originated [87] around the same time window that Hanseniaspora did (Fig 1B), leading us to speculate that the evolutionary trigger of Hanseniaspora hypermutation could have been adaptation to the grape environment.…”

Section: Discussionmentioning

confidence: 85%

Extensive loss of cell-cycle and DNA repair genes in an ancient lineage of bipolar budding yeasts

et al. 2019

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Cell-cycle checkpoints and DNA repair processes protect organisms from potentially lethal mutational damage. Compared to other budding yeasts in the subphylum Saccharomycotina, we noticed that a lineage in the genus Hanseniaspora exhibited very high evolutionary rates, low Guanine–Cytosine (GC) content, small genome sizes, and lower gene numbers. To better understand Hanseniaspora evolution, we analyzed 25 genomes, including 11 newly sequenced, representing 18/21 known species in the genus. Our phylogenomic analyses identify two Hanseniaspora lineages, a faster-evolving lineage (FEL), which began diversifying approximately 87 million years ago (mya), and a slower-evolving lineage (SEL), which began diversifying approximately 54 mya. Remarkably, both lineages lost genes associated with the cell cycle and genome integrity, but these losses were greater in the FEL. E.g., all species lost the cell-cycle regulator WHIskey 5 ( WHI5 ), and the FEL lost components of the spindle checkpoint pathway (e.g., Mitotic Arrest-Deficient 1 [ MAD1 ], Mitotic Arrest-Deficient 2 [ MAD2 ]) and DNA-damage–checkpoint pathway (e.g., Mitosis Entry Checkpoint 3 [ MEC3 ], RADiation sensitive 9 [ RAD9 ]). Similarly, both lineages lost genes involved in DNA repair pathways, including the DNA glycosylase gene 3-MethylAdenine DNA Glycosylase 1 ( MAG1 ), which is part of the base-excision repair pathway, and the DNA photolyase gene PHotoreactivation Repair deficient 1 ( PHR1 ), which is involved in pyrimidine dimer repair. Strikingly, the FEL lost 33 additional genes, including polymerases (i.e., POLymerase 4 [ POL4 ] and POL32 ) and telomere-associated genes (e.g., Repressor/activator site binding protein-Interacting Factor 1 [ RIF1 ], Replication Factor A 3 [ RFA3 ], Cell Division Cycle 13 [ CDC13 ], Pbp1p Binding Protein [ PBP2 ]). Echoing these losses, molecular evolutionary analyses reveal that, compared to the SEL, the FEL stem lineage underwent a burst of accelerated evolution, which resulted in greater mutational loads, homopolymer instabilities, and higher fractions of mutations associated with the common endogenously damaged base, 8-oxoguanine. We conclude that Hanseniaspora is an ancient lineage that has diversified and thrived, despite lacking many otherwise highly conserved cell-cycle and genome integrity genes and pathways, and may represent a novel, to our knowledge, system for studying cellular life without them.

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“…Today, 10 strains belonging to six different Hanseniaspora species have been completely sequenced [40][41][42][43]. The data collected from these sequences (Table 1) evidence the close relation between H. vineae and H. osmophila that present a similar genome size and G + C percentage compared with the others.…”

Section: Comparative Analysis Of Hanseniaspora Genomesmentioning

confidence: 89%

Oenological Impact of the Hanseniaspora/Kloeckera Yeast Genus on Wines—A Review

et al. 2018

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Apiculate yeasts of the genus Hanseniaspora/Kloeckera are the main species present on mature grapes and play a significant role at the beginning of fermentation, producing enzymes and aroma compounds that expand the diversity of wine color and flavor. Ten species of the genus Hanseniaspora have been recovered from grapes and are associated in two groups: H. valbyensis, H. guilliermondii, H. uvarum, H. opuntiae, H. thailandica, H. meyeri, and H. clermontiae; and H. vineae, H. osmophila, and H. occidentalis. This review focuses on the application of some strains belonging to this genus in co-fermentation with Saccharomyces cerevisiae that demonstrates their positive contribution to winemaking. Some consistent results have shown more intense flavors and complex, full-bodied wines, compared with wines produced by the use of S. cerevisiae alone. Recent genetic and physiologic studies have improved the knowledge of the Hanseniaspora/Kloeckera species. Significant increases in acetyl esters, benzenoids, and sesquiterpene flavor compounds, and relative decreases in alcohols and acids have been reported, due to different fermentation pathways compared to conventional wine yeasts.

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Genome Sequence of the Nonconventional Wine Yeast Hanseniaspora guilliermondii UTAD222

Abstract: In this work, we disclose the genome sequence and a corresponding manually curated annotation of the non-Saccharomyces yeast Hanseniaspora guilliermondii UTAD222, a strain shown to have interesting oenological traits for the production of wines with improved aromatic properties.

Cited by 9 publications

References 9 publications

Genome Sequencing and Comparative Analysis of Three Hanseniaspora uvarum Indigenous Wine Strains Reveal Remarkable Biotechnological Potential

Genome Sequencing and Comparative Analysis of Three Hanseniaspora uvarum Indigenous Wine Strains Reveal Remarkable Biotechnological Potential

Extensive loss of cell-cycle and DNA repair genes in an ancient lineage of bipolar budding yeasts

Oenological Impact of the Hanseniaspora/Kloeckera Yeast Genus on Wines—A Review

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