Genome Sequence of the Mosquitocidal Bacillus thuringiensis Strain BR58, a Biopesticide Product Effective against the Coffee Berry Borer (
            <i>Hypothenemus hampei</i>
            )

Zorzetti, Janaina; Ricietto, Ana Paula Scaramal; Silva, Carlos R. M. da; Wolf, Ivan Rodrigo; Vilas-Bôas, Gislayne Trindade; Neves, Pedro Manuel Oliveira Janeiro; Meneguim, Ana M.; Vilas-Bôas, Laurival A.

doi:10.1128/genomea.01232-15

Cited by 4 publications

(5 citation statements)

References 7 publications

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“… 2015 ; Zorzetti et al. 2015 ). In addition, strain 147 is the only natural strain from this group that lacks the pGIL01 prophage (see below and Gillis and Mahillon 2014c ).…”

Section: Bacillus Thuringiensis Sv Israelensis mentioning

confidence: 99%

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Role of plasmid plasticity and mobile genetic elements in the entomopathogen Bacillus thuringiensis serovar israelensis

Gillis

Fayad

Makart

et al. 2018

FEMS Microbiology Reviews

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Bacillus thuringiensis is a well-known biopesticide that has been used for more than 80 years. This spore-forming bacterium belongs to the group of Bacillus cereus that also includes, among others, emetic and diarrheic pathotypes of B. cereus, the animal pathogen Bacillus anthracis and the psychrotolerant Bacillus weihenstephanensis. Bacillus thuringiensis is rather unique since it has adapted its lifestyle as an efficient pathogen of specific insect larvae. One of the peculiarities of B. thuringiensis strains is the extent of their extrachromosomal pool, with strains harbouring more than 10 distinct plasmid molecules. Among the numerous serovars of B. thuringiensis, ‘israelensis’ is certainly emblematic since its host spectrum is apparently restricted to dipteran insects like mosquitoes and black flies, vectors of human and animal diseases such as malaria, yellow fever, or river blindness. In this review, the putative role of the mobile gene pool of B. thuringiensis serovar israelensis in its pathogenicity and dedicated lifestyle is reviewed, with specific emphasis on the nature, diversity, and potential mobility of its constituents. Variations among the few related strains of B. thuringiensis serovar israelensis will also be reported and discussed in the scope of this specialised insect pathogen, whose lifestyle in the environment remains largely unknown.

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“… 2015 ; Zorzetti et al. 2015 ). In addition, strain 147 is the only natural strain from this group that lacks the pGIL01 prophage (see below and Gillis and Mahillon 2014c ).…”

Section: Bacillus Thuringiensis Sv Israelensis mentioning

confidence: 99%

“… 2015 ; Zorzetti et al. 2015 ) containing all other B. thuringiensis sv. israelensis -specific plasmids, except for pXO16, as previously indicated (Table 2 ).…”

Section: The Extrachromosomal Zoomentioning

confidence: 99%

Role of plasmid plasticity and mobile genetic elements in the entomopathogen Bacillus thuringiensis serovar israelensis

Gillis

Fayad

Makart

et al. 2018

FEMS Microbiology Reviews

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“…Whole-genome sequencing was performed by Illumina Hiseq sequencing and the paired-end sequence strategy was chosen, which generated a total of 3,042,174 reads of high quality. The analysis methods were performed according to Zorzetti et al . (2015 ).…”

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confidence: 99%

Complete genome sequence of Bacillus thuringiensis BR145, a strain with insecticidal activity against Lepidoptera pests

et al. 2022

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Bacillus thuringiensis BR145 isolated from a soybean field in Southern Brazil showed toxicity against two important insect pests from soybean crop, Helicoverpa armigera, and Chrysodeixis includens, with LC 50 0.294 µg.cm -2 and 0.277 µg.cm -2 , respectively. We analyzed the genome of this strain through sequences obtained by Next Generation DNA Sequencing and de novo assembly. The analysis of the genome revealed insecticidal genes cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Ab, cyt1, and vip3Aa , suggesting the use of this strain in new strategies of biological control.

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“…During sporulation, the gram-positive bacterium Bacillus thuringiensis (Bt) produces crystalline inclusions consisting of δ-endotoxins (Cry or Cyt proteins) with insecticidal activity 1 . Genomic analysis has contributed to the identification of new genes coding for toxins that are active against different insect species including orders such as Lepidoptera, Diptera [2][3][4][5][6][7] , and Coleoptera 8 . Proteins with nematicidal [9][10][11] and molluscicidal 12 activities have also been described.…”

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confidence: 99%

“…Sequencing of complete Bt genomes has allowed structural and functional analysis of new plasmids that enhance our knowledge of the pathogenic properties of Bt in targeting organisms [14][15][16][17] . One study reported the plasmid sequence of a Bacillus thuringiensis serovar israelensis (Bti) strain, and revealed that it may produce up to seven crystal-forming toxins, named Cry4A, Cry4B, Cry10A, Cry11A, Cyt1A, Cyt2Ba, and Cyt1Ca, which are all encoded by genes found in a single 127923 bp plasmid called pBtoxis 18 .…”

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confidence: 99%

Comparative genomic analysis and mosquito larvicidal activity of four Bacillus thuringiensis serovar israelensis strains

Alves

Melo

Oliveira

et al. 2020

Sci Rep

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Bacillus thuringiensis serovar israelensis (Bti) is used to control insect vectors of human and animal diseases. in the present study, the toxicity of four strains of Bti, named T0124, T0131, T0137, and T0139, toward Aedes aegypti and Culex quinquefasciatus larvae was analyzed. The T0131 strain showed the highest larvicidal activity against A. aegypti (Lc 50 = 0.015 µg/ml) and C. quinquefasciatus larvae (Lc 50 = 0.035 µg/ml) when compared to the other strains. Furthermore, the genomic sequences of the four strains were obtained and compared. These Bti strains had chromosomes sizes of approximately 5.4 Mb with GC contents of ~35% and 5472-5477 putative coding regions. Three small plasmids (5.4, 6.8, and 7.6 kb) and three large plasmids (127, 235, and 359 kb) were found in the extrachromosomal content of all four strains. the Snp-based phylogeny revealed close relationship among isolates from this study and other Bti isolates, and SNPs analysis of the plasmids 127 kb did not reveal any mutations in δ-endotoxins genes. This newly acquired sequence data for these Bti strains may be useful in the search for novel insecticidal toxins to improve existing ones or develop new strategies for the biological control of important insect vectors of human and animal diseases.

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Genome Sequence of the Mosquitocidal Bacillus thuringiensis Strain BR58, a Biopesticide Product Effective against the Coffee Berry Borer ( Hypothenemus hampei )

Cited by 4 publications

References 7 publications

Role of plasmid plasticity and mobile genetic elements in the entomopathogen Bacillus thuringiensis serovar israelensis

Role of plasmid plasticity and mobile genetic elements in the entomopathogen Bacillus thuringiensis serovar israelensis

Complete genome sequence of Bacillus thuringiensis BR145, a strain with insecticidal activity against Lepidoptera pests

Comparative genomic analysis and mosquito larvicidal activity of four Bacillus thuringiensis serovar israelensis strains

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