Genome sequence of the Medicago-nodulating Ensifer meliloti commercial inoculant strain RRI128

Reeve, Wayne; Ballard, Ross; Drew, Elizabeth; Tian, Rui; Bräu, Lambert; Goodwin, Lynne; Han, James; Reddy, T. B. K.; Chen, Amy; Mavrommatis, Konstantinos; Markowitz, Victor; Palaniappan, Krishna; Ivanova, Natalia; Pati, Amrita; Woyke, Tanja; Kyrpides, Nikos C.

doi:10.4056/sigs.4929626

Cited by 4 publications

(1 citation statement)

References 36 publications

(40 reference statements)

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“…In these countries, E. meliloti strain RRI128 is used as a commercial inoculant (21) . This strain, which establishes symbiosis with perennial and annual Medicago species, was isolated from a nodule from the roots of barrel medic (M. truncatula) grown in a greenhouse in Victoria soil of Australia (68) . However, its origin is unclear and it is believed that it was isolated for the first time in 1995 in New Zealand (69) .…”

Section: Selection Of Rhizobia Strains For Development Of Alfalfa Inomentioning

confidence: 99%

Rhizobium inoculants for alfalfa in acid soils: A proposal for Uruguay

et al. 2019

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Ensifer meliloti establishes symbiosis with Medicago sativa (alfalfa) and other perennial species of Medicago that grow in soils with neutral to alkaline pH, whereas Ensifer medicae makes symbiosis with annual medics adapted to moderately acid soils. The new species Rhizobium favelukesii, whose strain is LPU83, belongs to an alfalfa group of inefficient rhizobia, known as the Oregon type, initially represented by Rhizobium sp. strain Or191. R. favelukesii is considered a potential risk in the acid soils where alfalfa is grown, and could explain the inefficient nodulation observed in different countries. In acidic soils from the «Dairy Basin» of Uruguay, producers inoculate alfalfa with E. melliloti U143 strain. This edaphic condition is often marginal because the maximum potential of rhizobia-alfalfa symbiosis is not achieved at acid pH. Although Uruguay has an outstanding position in the production and use of rhizobial inoculants, the commercial strains currently used in Trifolium, Lotus and alfalfa were selected about 50 years ago in different conditions that the present ones as a consequence of: i) the displacement of cultivated pastures to other sites, ii) the sowing method, and iii) the use of new cultivars. In this review, alfalfa inoculation is analyzed in some countries and a strategy for the development of an inoculant suitable for Uruguayan acid soils is proposed. This strategy is based on the selection of efficient and competitive strains, as the first selection criteria, and persistency in soil as the second one.

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Section: Selection Of Rhizobia Strains For Development Of Alfalfa Inomentioning

confidence: 99%

Rhizobium inoculants for alfalfa in acid soils: A proposal for Uruguay

et al. 2019

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Isolation and characterisation of endophytic actinobacteria and their effect on the early growth and nodulation of lucerne (Medicago sativa L.)

Franco

Ballard

et al. 2015

Plant Soil

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Sensitivity of the messina (

Ballard

Peck

2021

Crop & Pasture Science

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Messina (Melilotus siculus) is a new annual pasture legume with better combined waterlogging and salt tolerance than other annual legumes. Messina cv. Neptune and a new salt-tolerant rhizobial symbiont (Sinorhizobium medicae SRDI-554) were made available to Australian growers in 2017. Messina is related to the annual medics (Medicago spp.) that are nodulated by the same genus of rhizobia and regarded as sensitive to soil acidity. Because some saltland soils are acidic, it is important to understand the sensitivity of messina to soil acidity in order to avoid failures during early adoption. Acidity tolerance of the messina–Sinorhizobium symbiosis was investigated in a hydroponic experiment (inoculation with SRDI-554, or the salt-intolerant strain WSM-1115 recommended for medics), and in three acidic soils (pHCa 4.3–5.5) (inoculation with SRDI-554 ± lime pelleting of seed), in the greenhouse. In the hydroponic experiment, the percentage of messina plants (with SRDI-554) that formed nodules declined at pH levels between 5.7 (43%) and 5.5 (4%). Strain SRDI-554 was slightly more sensitive to acidity than strain WSM-1115. In the acidic soils, more plants formed nodules than in the hydroponic experiment at similar pH levels; however, without lime pelleting, nodule number was inadequate at soil pHCa <5.5. Addition of lime to seed was beneficial to messina nodulation. Nodule number per plant increased from 4.0 to 9.6 with the addition of lime. The messina–Sinorhizobium symbiosis was confirmed as sensitive to low pH. At pHCa 5.5, which is the level recommended as the lower limit for growing messina, nodule number was constrained in both hydroponics and soil. The risk of suboptimal nodulation would be reduced if the recommended lower soil pH limit for growing messina is increased to pHCa 5.8, in line with most annual medics. Efforts to improve the acidity tolerance of the messina symbiosis would be best focused on the rhizobial symbiont, rather than the plant.

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Genome sequence of the Medicago-nodulating Ensifer meliloti commercial inoculant strain RRI128

Cited by 4 publications

References 36 publications

Rhizobium inoculants for alfalfa in acid soils: A proposal for Uruguay

Rhizobium inoculants for alfalfa in acid soils: A proposal for Uruguay

Isolation and characterisation of endophytic actinobacteria and their effect on the early growth and nodulation of lucerne (Medicago sativa L.)

Sensitivity of the messina (

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