Genome Sequence of the Curdlan-Producing Agrobacterium sp. Strain ATCC 31749

Ruffing, Anne; Castro-Melchor, Marlene; Hu, Wei Shou; Chen, Rachel R.

doi:10.1128/jb.05302-11

Cited by 28 publications

(12 citation statements)

References 18 publications

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“…The lack of experimental characterization of curdlan synthase makes it difficult to determine its mechanism of biosynthesis and secretion ( Whitney and Howell, 2013 ). Many genomic as well as transcriptomic and proteomic information is available for curdlan producing Agrobacterium strains ( McIntosh et al, 2005 ; Zheng et al, 2007 ; Jin et al, 2008 ; Wu et al, 2008 ; Ruffing and Chen, 2010 , 2012 ; Ruffing et al, 2011 ; Jin and Lee, 2014 ). Results as obtained by that approaches displayed metabolic structures and pathway distributions indicating that energy efficiency, rather than substrate availability is the major constraint for improving curdlan yield ( Zheng et al, 2007 ).…”

Section: Exopolysaccharides Produced Via the Various Synthase-dependementioning

confidence: 99%

Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies

2015

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Bacteria produce a wide range of exopolysaccharides which are synthesized via different biosynthesis pathways. The genes responsible for synthesis are often clustered within the genome of the respective production organism. A better understanding of the fundamental processes involved in exopolysaccharide biosynthesis and the regulation of these processes is critical toward genetic, metabolic and protein-engineering approaches to produce tailor-made polymers. These designer polymers will exhibit superior material properties targeting medical and industrial applications. Exploiting the natural design space for production of a variety of biopolymer will open up a range of new applications. Here, we summarize the key aspects of microbial exopolysaccharide biosynthesis and highlight the latest engineering approaches toward the production of tailor-made variants with the potential to be used as valuable renewable and high-performance products for medical and industrial applications.

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Section: Exopolysaccharides Produced Via the Various Synthase-dependementioning

confidence: 99%

Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies

2015

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“…The genome sequence of ATCC 31749 has been determined to better understand how curdlan biosynthesis is regulated [37]. The genes involved in curdlan biosynthesis have been mapped to two loci in ATCC 31749 [38].…”

Section: Genetics and Regulation Of Curdlan Biosynthesismentioning

confidence: 99%

Production of the Polysaccharide Curdlan by Agrobacterium species on Processing Coproducts and Plant Lignocellulosic Hydrolysates

West

2020

Fermentation

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This review examines the production of the biopolymer curdlan, synthesized by Agrobacterium species (sp.), on processing coproducts and plant lignocellulosic hydrolysates. Curdlan is a β-(1→3)-D-glucan that has various food, non-food and biomedical applications. A number of carbon sources support bacterial curdlan production upon depletion of nitrogen in the culture medium. The influence of culture medium pH is critical to the synthesis of curdlan. The biosynthesis of the β-(1→3)-D-glucan is likely controlled by a regulatory protein that controls the genes involved in the bacterial production of curdlan. Curdlan overproducer mutant strains have been isolated from Agrobacterium sp. ATCC 31749 and ATCC 31750 by chemical mutagenesis and different selection procedures. Several processing coproducts of crops have been utilized to support the production of curdlan. Of the processing coproducts investigated, cassava starch waste hydrolysate as a carbon source or wheat bran as a nitrogen source supported the highest curdlan production by ATCC 31749 grown at 30 °C. To a lesser extent, plant biomass hydrolysates have been explored as possible substrates for curdlan production by ATCC 31749. Prairie cordgrass hydrolysates have been shown to support curdlan production by ATCC 31749 although a curdlan overproducer mutant strain, derived from ATCC 31749, was shown to support nearly double the level of ATCC 31749 curdlan production under the same growth conditions.

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“…Genome sequences were then annotated with the MicroScope platform (Vallenet et al 2013) (Goodner et al 2001;Wood et al 2001;Li et al 2011;Ruffing et al 2011;Wibberg et al 2011;Hao, Lin, et al 2012;Hao, Xie, et al 2012). These 22 genomes covered 10 closely related but genomically differentiated species (G1 to G9 and G13), with up to five isolates per species.…”

Section: Bacterial Culture Experimentsmentioning

confidence: 99%

Ancestral genome estimation reveals the history of ecological diversification inAgrobacterium

Lassalle

Planel

Penel

et al. 2015

Preprint

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Horizontal gene transfer (HGT) is considered as a major source of innovation in bacteria, and as such is expected to drive adaptation to new ecological niches. However, among the many genes acquired through HGT along the diversification history of genomes, only a fraction may have actively contributed to sustained ecological adaptation. We used a phylogenetic approach accounting for the transfer of genes (or groups of genes) to estimate the history of genomes in Agrobacterium biovar 1, a diverse group of soil and plantdwelling bacterial species. We identified clade-specific blocks of co-transferred genes encoding coherent biochemical pathways that may have contributed to the evolutionary success of key Agrobacterium clades.This pattern of gene co-evolution rejects a neutral model of transfer, in which neighbouring genes would be transferred independently of their function and rather suggests purifying selection on collectively coded acquired pathways. The acquisition of these synapomorphic blocks of co-functioning genes probably drove the ecological diversification of Agrobacterium and defined features of ancestral ecological niches, which consistently hint at a strong selective role of host plant rhizospheres.

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Genome Sequence of the Curdlan-Producing Agrobacterium sp. Strain ATCC 31749

Cited by 28 publications

References 18 publications

Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies

Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies

Production of the Polysaccharide Curdlan by Agrobacterium species on Processing Coproducts and Plant Lignocellulosic Hydrolysates

Ancestral genome estimation reveals the history of ecological diversification inAgrobacterium

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